In Vitro Cytotoxic and Anthelmintic Activities of <i>Leonurus sibiricus L.</i>

Abstract

This study investigated the cytotoxic and anthelmintic activities of Leonurus sibiricus L.(commonly known as Raktodrone in Bangladesh) belonging to the family Labiatae. The dried leaves and roots of L. sibiricus were extracted with methanol and fractionated by modified Kupchan method. The crude methanolic extracts as well as its soluble fractions of petroleum ether, ethyl acetate and chloroform were screened for cytotoxic activity using brine shrimp lethality bioassay. They were found to possess significant cytotoxic activities. The LC 50 values of crude extract of leaves and its pet-ether, ethyl acetate and chloroform soluble fractions were 1.0, 2.0, 2.11 and1.33 µg/ml, respectively. On the other hand, the LC 50 of crude methanolic extract of roots and fractions of pet-ether, ethyl acetate and chloroform were 2.0µg/ml, 2.81 µg/ml, 3.55 µg/ml and 7.58µg/ml, respectively. Vincristine sulphate was used as positive control. The crude methanol extract of leaves and roots also showed very good anthelmintic activities as determined against the earthworms, Pheretima posthuma. The study confirms the moderate anthelmintic and potent cytotoxic activities of leaf and root extracts of L. sibiricus.

Anthelmintic activity of silver-extract nanoparticles synthesized from the combination of silver nanoparticles and M. charantia fruit extract

“…Because of easy availability, earthworms have been used widely for the initial evaluation of anthelmintic compounds in vitro [10]. Adult earthworms were collected from the wet soil of Noakhali district of Bangladesh.…”

Thrombolytic and Antimicrobial Activities of Andrographis paniculata – A Preliminary Investigation

“…1) filter paper and concentrated with a rotary evaporator. An aliquot of the concentrated ethanol extract was fractionated by the modified Kupchan method (Saha et al, 2012) and the resultant partitioned were evaporated to dryness with a rotary evaporator to yield hexane, carbon tetrachloride and chloroform soluble materials. The residues were then stored in a refrigerator until further use.…”

Biochemical Analysis of Leonurus Sibiricus and Prediction of Its Bioactive Compound-Protein Interactions in Homo Sapiens

“…The holistic nature of herbal medicine, which offers comprehensive solutions by addressing underlying systemic imbalances, has contributed to its growing popularity. Accordingly, the discovery and development of new chemical substances for controlling diabetes, CVD, cancer are greatly needed and it has been promoted studies of traditionally used medicinal plants, which are generally considered to be very important sources of bioactive substances (Saha et al, 2012). Traditional medicinal plants demonstrate the beneficial outcomes from a long time without having recognized molecular basis of mode of actions.…”