In vitro cytotoxicity MTT assay in THP-1 cell lines study of crude extract of herbal mixtures (HS1 and HS2) used in the treatment of tuberculosis

Jyoti, Tomar; Rastogi, Vijaylatha; Patni, Tarun

doi:10.1016/j.ijmmb.2021.08.286

Cited by 1 publication

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“…The Thiazolyl blue tetrazolium bromide colorimetric assay is commonly used as one of the methods to assess cytotoxicity ( Jyoti et al, 2021 ), and from Figure 3A , cell viability gradually increases at fermentation liquid concentrations of 0.5 to 2%. Above 2%, cell survival gradually decreased.…”

Section: Resultsmentioning

confidence: 99%

Improvement of antioxidant activity and active ingredient of Dendrobium officinale via microbial fermentation

Xie²,

Su³

et al. 2023

Front. Microbiol.

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This study used brewer’s yeast to ferment Dendrobium officinale and single-factor and orthogonal experiments were conducted to determine the optimal fermentation conditions. The antioxidant capacity of Dendrobium fermentation solution was also investigated by in vitro experiments, which showed that different concentrations of fermentation solution could effectively enhance the total antioxidant capacity of cells. The fermentation liquid was found to contain seven sugar compounds including glucose, galactose, rhamnose, arabinose, and xylose using gas chromatography–mass spectrometry (GC–MS) and high performance liquid chromatography-quadrupole-time of flight mass spectrometry (HPLC-Q-TOF-MS), with the highest concentrations of glucose and galactose at 194.628 and 103.899 μg/ml, respectively. The external fermentation liquid also contained six flavonoids with apigenin glycosides as the main structure and four phenolic acids including gallic acid, protocatechuic acid, catechol, and sessile pentosidine B.

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Section: Resultsmentioning

confidence: 99%