In vitro cytotoxicity of GO&amp;ndash;DOx on FaDu squamous carcinoma cell lines

Singh, Manjri; Gupta, Parul; Baronia, Richa; Singh, Priti; Karuppiah, Stalin; Shankar, Rishi; Dwivedi, Premendra D.; Singh, Surinder P.

doi:10.2147/ijn.s124891

Cited by 9 publications

(4 citation statements)

References 6 publications

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“…Both cell lines showed distinct size and morphology alteration compared to the untreated control. Cellular shrinkage and proliferation inhibition were observed in CAG treatment groups, which increased with higher CAG concentration, suggesting a dose-dependent trend [121, 122]. Furthermore, morphological changes were studied with starting raw material GO, and traditional citrate-synthesized nanocomposite rGO-AuNPs, as a comparative approach (S2 Fig).…”

Section: Resultsmentioning

confidence: 99%

Hybrid nanocomposite curcumin-capped gold nanoparticle-reduced graphene oxide: Anti-oxidant potency and selective cancer cytotoxicity

et al. 2019

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Nanotechnology-based antioxidants and therapeutic agents are believed to be the next generation tools to face the ever-increasing cancer mortality rates. Graphene stands as a preferred nano-therapeutic template, due to the advanced properties and cellular interaction mechanisms. Nevertheless, majority of graphene-based composites suffer from hindered development as efficient cancer therapeutics. Recent nano-toxicology reviews and recommendations emphasize on the preliminary synthetic stages as a crucial element in driving successful applications results. In this study, we present an integrated, green, one-pot hybridization of target-suited raw materials into curcumin-capped gold nanoparticle-conjugated reduced graphene oxide (CAG) nanocomposite, as a prominent anti-oxidant and anti-cancer agent. Distinct from previous studies, the beneficial attributes of curcumin are employed to their fullest extent, such that they perform dual roles of being a natural reducing agent and possessing antioxidant anti-cancer functional moiety. The proposed novel green synthesis approach secured an enhanced structure with dispersed homogenous AuNPs (15.62 ± 4.04 nm) anchored on reduced graphene oxide (rGO) sheets, as evidenced by transmission electron microscopy, surpassing other traditional chemical reductants. On the other hand, safe, non-toxic CAG elevates biological activity and supports biocompatibility. Free radical DPPH inhibition assay revealed CAG antioxidant potential with IC 50 (324.1 ± 1.8%) value reduced by half compared to that of traditional citrate-rGO-AuNP nanocomposite (612.1 ± 10.1%), which confirms the amplified multi-potent antioxidant activity. Human colon cancer cell lines (HT-29 and SW-948) showed concentration- and time-dependent cytotoxicity for CAG, as determined by optical microscopy images and WST-8 assay, with relatively low IC 50 values (~100 μg/ml), while preserving biocompatibility towards normal human colon (CCD-841) and liver cells (WRL-68), with high selectivity indices (≥ 2.0) at all tested time points. Collectively, our results demonstrate effective green synthesis of CAG nanocomposite, free of additional stabilizing agents, and its bioactivity as an antioxidant and selective anti-colon cancer agent.

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Section: Resultsmentioning

confidence: 99%

Hybrid nanocomposite curcumin-capped gold nanoparticle-reduced graphene oxide: Anti-oxidant potency and selective cancer cytotoxicity

et al. 2019

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“…Consequently, GO-DOX would then be able to enter the tumor tissue through the ruptured blood vessels. In an acidic environment, which is present in tumor cells, DOX is released from GO [65] and enters the cell nucleus to promote apoptosis [73]. This method helps GO-DOX penetrate the tumor vascular barrier and enter the tumor tissue.…”

Section: Journal Of Nanomaterialsmentioning

confidence: 99%

Cavitation-Enhanced Delivery of the Nanomaterial Graphene Oxide-Doxorubicin to Hepatic Tumors in Nude Mice Using 20 kHz Low-Frequency Ultrasound and Microbubbles

Shen

et al. 2020

Journal of Nanomaterials

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Graphene oxide (GO) is a kind of nanomaterial. Here, we explored its application in tumor treatment. We loaded doxorubicin onto the surface of GO to form graphene oxide-doxorubicin (GO-DOX). After injection of the contrast agent SonoVue microbubbles (MBs) into the tail vein of tumor-bearing nude mice, subcutaneous hepatic carcinomas inoculated with the HepG2 cell line were irradiated with 20 kHz low-frequency ultrasound (US). Subsequently, GO-DOX was injected into the tail vein of nude mice. Transmission electron microscopy (TEM) and TUNEL assays were performed to observe the curative effects. Biocompatibility tests of GO-DOX included routine blood cell counts, blood smears, serum biochemical assays, and histological sampling of important organs. It was found that the nanomaterial GO-DOX promoted apoptosis of tumor cells in nude mice. TEM of the USMB+GO-DOX treatment showed vascular endothelial cell wall rupture, widened endothelial cell gaps, black granules in the vascular lumen, interstitial erythrocyte leakage, and apparent apoptosis of tumor cells. There were no toxic side effects of GO-DOX on the blood system and in the major organs of these mice. Ultrasound cavitation destroys tumor blood vessels and enhances the release of nanomaterials to tumor cells of nude mice.

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“…此外, 有机小分子化合物还可以通过非共价键作用对 GO 进行表面修饰, 如静电力、范德华力、氢键和π-π堆积作用等 [15] . 通过π-π堆积作用, 可以将具有芳香环结构的抗癌药物修饰到 GO 表面(图 3), 如阿霉素(DOX) [64,65] 和喜树碱(CPT) [66,67] , 进而将药物递送到肿瘤细胞与组织 [68] . 例如, 利用 π-π 堆积和疏水作用将 DOX 装载到转铁蛋白(Tf)与 PEG 修饰的 GO (Tf-PEG-GO)表面, 装载效率可达 115.4%, 对大鼠 C6 神经胶质瘤具有良好的靶向治疗效果 [69] .…”

Section: N-(3-二甲氨丙基)-n'-乙基碳二亚胺(edc)/n-羟基丁二unclassified

“…图 3 GO 与 DOX 通过π-π堆积作用结合的示意图 [68] Figure 3 Schematic representation for a GO-DOX conjugation through π-π stacking. Reprinted with permission from ref.…”

Section: N-(3-二甲氨丙基)-n'-乙基碳二亚胺(edc)/n-羟基丁二mentioning

confidence: 99%

Surface Chemical Modifications of Graphene Oxide and Interaction Mechanisms at the Nano-Bio Interface

Ma¹,

Xu²,

Liu³

2020

Acta Chim. Sinica

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Due to the unique physicochemical properties, graphene oxide has been widely applied in material chemistry, biomedical science and life science. However, here is still a great challenge to maximize the advantages of graphene oxide and overcome the deleterious effects caused by its inherent properties. For a better understanding of current status in this research field, recent progress in surface chemical modifications of graphene oxide and interaction mechanisms at the nano-bio interface has been comprehensively reviewed. First, the physicochemical properties of graphene oxide and the representative strategies of surface chemical modifications will be briefly introduced, including oxidation and reduction, carboxylation, amination, small organic molecule modification, polymer modification, peptide/protein modification, nucleic acid modification and nanoparticle modification, as well as their potential roles in mediating the graphene oxide-resulted biological effects. Following, we will present the primary interaction mechanisms of pristine and surface-modified graphene oxide at the nano-bio interface, including the formation of protein corona, cell membrane damage, membrane receptor interaction and oxidative stress. Finally, the knowledge gaps and future challenges in this research field will be detailedly discussed. Keywords graphene oxide; surface chemical modification; nano-bio interface; interaction mechanism; biological effect 1 引言 2004 年, Geim 等 [1] 首次通过机械剥离法制得单层石墨烯. 石墨烯(graphene)是一种二维纳米材料, 具有类似蜂巢的六元环平面网状结构, 厚度仅为 0.3354 nm [1] . 在石墨烯的纳米片层结构中, 碳原子发生了 sp 2 杂化, 每个碳原子通过键长为 142 nm 的 σ 键与其它三个碳原子连接, 并在与片层垂直的方向形成离域大 π 键 [2,3] . 这些结构特征赋予了石墨烯优异的机械强度、化学稳定性、高比表面积、导电和导热性能 [4][5][6][7] . 因此, 石墨烯在能源、材料、健康、环境等方面都展现了广阔的应用前景.石墨烯拥有多种衍生物, 包括氧化石墨烯(graphene oxide, GO)、还原氧化石墨烯(reduced graphene oxide, rGO)、氟化石墨烯(fluorographene)等.

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In vitro cytotoxicity of GO–DOx on FaDu squamous carcinoma cell lines

Cited by 9 publications

References 6 publications

Hybrid nanocomposite curcumin-capped gold nanoparticle-reduced graphene oxide: Anti-oxidant potency and selective cancer cytotoxicity

Hybrid nanocomposite curcumin-capped gold nanoparticle-reduced graphene oxide: Anti-oxidant potency and selective cancer cytotoxicity

Cavitation-Enhanced Delivery of the Nanomaterial Graphene Oxide-Doxorubicin to Hepatic Tumors in Nude Mice Using 20 kHz Low-Frequency Ultrasound and Microbubbles

Surface Chemical Modifications of Graphene Oxide and Interaction Mechanisms at the Nano-Bio Interface

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