In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system

Sepúlveda, Soledad; García, Javier I.; Arriaga, Elard; Díaz, J. Hernández; Noriega-Portella, Luis; Noriega-Hoces, Luis

doi:10.1016/j.fertnstert.2008.02.169

Cited by 83 publications

(67 citation statements)

References 19 publications

(20 reference statements)

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“…It is likely that embryos cultured in single media reached the 5-cell stage faster than the sequential system as the initial mitosis cycle was shorter. Two comparative studies reported higher day5 blastocyst yield in single media [10,13] and another did not find any difference [11] which was in accordance to the results of the present study. However, it is noteworthy to mention that although the overall utilization rate was similar, significantly more day6 blastocysts have been cryopreserved in the sequential culture system than single media.…”

Section: Discussionsupporting

confidence: 92%

“…However, it is noteworthy to mention that although the overall utilization rate was similar, significantly more day6 blastocysts have been cryopreserved in the sequential culture system than single media. This finding can be interpreted as equalization of embryo utilization to be compensated by developmentally delayed blastocysts in the sequential system, justifying the findings of Reed et al [10] and Sepulveda et al [13].…”

Section: Discussionsupporting

confidence: 74%

“…Other studies utilizing various accessible media randomized patients [11,12] or donors [13] into groups. The results supported single media approach due to a higher proportion of compacted day3 embryos to zygotes [13], a higher utilization rate at day3 [12], a higher morula formation at 4 [13], a higher blastocyst yield at day5 [10,13], and an increased implantation rate as compared to the sequential system [10,13]. Only in one study [11] there was not any difference in embryo development and clinical outcome.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study

Ciray¹,

Aksoy²,

Göktaş³

et al. 2012

J Assist Reprod Genet

145

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Objective To compare the dynamics of early development between embryos cultured in single and sequential media. Design Randomized, comparative study. Setting Private IVF centre. Patients A total of 446 metaphase II oocytes from 51 couples who underwent oocyte retrieval procedure for intracytoplasmic sperm injection. Forty-nine resulted in embryo transfer. Intervention Oocytes were split between single and sequential media produced by the same manufacturer and cultured in a time-lapse incubator. Main outcome measures Morphokinetic parameters until the embryos reached the 5-cell stage (t5), utilization, clinical pregnancy and implantation rates. Results Embryos cultured in single media were advanced from the first mitosis cycle and reached 2-to 5-cell stages earlier. There was not any difference between the durations for cell cycle two (cc20t3-t2) and s2 (t4-t3). The utilization, clinical pregnancy and implantation rates did not differ between groups. The proportion of cryopreserved day6 embryos to two pronuclei oocytes was significantly higher in sequential than in single media. Conclusions Morphokinetics of embryo development vary between single and sequential culture media at least until the 5-cell stage. The overall clinical and embryological parameters remain similar regardless of the culture system.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study

Ciray¹,

Aksoy²,

Göktaş³

et al. 2012

J Assist Reprod Genet

145

View full text Add to dashboard Cite

show abstract

“…Even though the improved culture media systems have increased the proportion of embryos that reach the blastocyst stage in vitro [41,49]. the proportion of embryos that reach this stage is still unpredictable [29,40,47], and some assisted reproduction centres are reluctant to adopt extended Capsule Older patients and patients with high BMI should not be included in extended embryo culture programmes.…”

Section: Introductionmentioning

confidence: 99%

“…Various factors, such as the culture medium characteristics [2,4,41], the laboratory conditions [23,27,46], the oocyte quality [6,18], and the sperm origin [32], may be responsible for this variation in the rate of blastocyst formation. However, the influence of the patient and cycle characteristics on blastocyst formation is not understood.…”

Section: Introductionmentioning

confidence: 99%

Patient selection criteria for blastocyst transfers in extended embryo culture programs

Braga

Setti

Figueira

et al. 2012

J Assist Reprod Genet

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Purpose To identify the correlation between different cycles, patient factors and blastocyst characteristics. Methods The study included 420 patients undergoing ICSI cycles and 2781 graded blastocysts, which took into account the blastocyst quality. The correlations between the blastocyst parameters and the patient and cycle characteristics were assessed. Results The blastocyst development was negatively correlated with the maternal age, BMI and dose of FSH. The ICM was negatively correlated with the FSH dose, whereas the TE quality was influenced by the FSH dose, the maternal age and the number of retrieved oocytes. The embryo morphology on days two and three may predict the blastocyst developmental competence. Conclusions Older patients and patients with high BMI should not be included in extended embryo culture programmes. The extended culture may not favour embryos with poor morphology on days two and three of development. Additionally, a lower ovarian stimulation and decreased oocyte yields may lead to the development of high-quality blastocysts.

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Culture media for human pre-implantation embryos in assisted reproductive technology cycles

Youssef

Mantikou

Wely

et al. 2015

Cochrane Database of Systematic Reviews

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In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system

Cited by 83 publications

References 19 publications

Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study

Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study

Patient selection criteria for blastocyst transfers in extended embryo culture programs

Culture media for human pre-implantation embryos in assisted reproductive technology cycles

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