In vitro development of sheep embryos derived of repeated laparoscopic folliculoaspiration

Sevillano, C.; Anel, L.; Fuente, J. de la; Alvarez, Maria Silvana Mendez; Celorrio, I.; Paz, P. de; Boixo, J.C.; Olmedo, J.A.

doi:10.1016/s0093-691x(97)82425-9

Cited by 7 publications

(4 citation statements)

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“…In vitro embryo production is one such technique with many advantages, which include repeated production of embryos from the same donor (Kuhholzer et al. 1997; Sevillano et al. 1997), use of prepuberal, puberal and pregnant animals as donors (Earl et al.…”

Section: Introductionmentioning

confidence: 99%

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Ovum Pick‐up in Sheep: a Comparison between Different Aspiration Devices for Optimal Oocyte Retrieval

Rodriguez

Anel

Alvarez

et al. 2006

Reprod Domestic Animals

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ContentsIn vivo ovum pick-up (OPU) in sheep may be improved with a proper choice of aspiration elements (needle and tubing) and aspiration vacuum pressure. In the present study, two experiments were carried out. In Expt 1, visible follicles in ovaries of slaughtered ewes (treated separately according to their diameters: small <3 mm, medium 3-5 mm and large >5 mm) were aspirated using different combinations of the three studied factors such as aspiration flow rate (10, 20, 30, 40 and 50 ml water/min), needle gauge (18 and 20 G) and tubing inner diameter (1, 2 or 3 mm internal diameter). In Expt 2, a study with two 18 G needles of different lengths (18 G: 82 mm; 18 GL: 600 mm) was carried out, using ovaries obtained postmortem, and performing in vivo laparoscopic follicular aspiration on ewes. We considered good quality oocytes as those with both complete compact cumulus and a homogeneous cytoplasm. Recovery rate, proportion of good quality oocytes (good quality oocytes/100 oocytes recovered) and overall efficiency (good quality oocytes/100 follicles aspirated) were noted. In Expt 1, aspiration flow rate affect remarkable proportion of good quality oocytes (69.5%, 50.5%, 44.8%, 36.5% and 28.3% for flows from 10 to 50 ml/min respectively, p < 0.05). Needle gauge did not affect aspiration device efficiency. Thin and intermediate tubings were more effective (overall efficiency rates: 34.9%, 32.3% and 28.1% for 1, 2 and 3 mm respectively, p < 0.05). Follicle size did not affect recovery rate, but proportion of good quality oocytes was higher for large (77.9%) and medium (64.4%) follicles (p < 0.05). Finally, some combinations of the aspiration device showed greater effectiveness. In Expt 2, needle length did not influence recovery rate, but good quality oocytes rate was significantly modified both post-mortem and in vivo (good quality rate for 18 G vs 18 GL needles: 69.5% vs 47.7% and 58.1% vs 25.4%, post-mortem and in vivo respectively, p < 0.05). We conclude that low-aspiration flow rates (10 and 20 ml/min) with thin or intermediate tubings (1 and 2 mm), and any short needle (18 G or 20 G) are the most adequate aspiration factors for OPU in sheep.

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Section: Introductionmentioning

confidence: 99%

“…1996; Anel et al. 1997, 1998; Sevillano et al. 1997) or flow rates between 6.5 and 10 ml water/min (Alvarez et al.…”

Section: Introductionmentioning

confidence: 99%

Ovum Pick‐up in Sheep: a Comparison between Different Aspiration Devices for Optimal Oocyte Retrieval

Rodriguez

Anel

Alvarez

et al. 2006

Reprod Domestic Animals

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“…However, Armstrong et al . (1992) (in the cow) and Sevillano et al . (1997) (in the sheep) reported that the potential of oocytes collected by LFA to develop to the blastocyst stage after in vitro fertilization was similar to that of IVM oocytes.…”

Section: Resultsmentioning

confidence: 99%

Caprine somatic cell nuclear transfer using in vivo matured oocytes collected by laparoscopic follicular aspiration

Ohkoshi

Takahashi

Koyama

et al. 2003

Animal Science Journal

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In vivo matured oocytes collected by laparoscopic follicular aspiration (LFA) from hormone treated female goats were used as recipient ooplasts for somatic cell nuclear transfer (SCNT). Japanese native (Shiba) goats were used as donor females and some donor females were used repeatedly (two or three times) at intervals of a few months. To induce synchronization of estrus, a sponge containing 0.5 g of progesterone was inserted into the vagina of each goat for 14 days. These animals were also treated with follicle stimulating hormone (FSH) in a series of 8 injections over 4 days. The first FSH injection was administered on the morning of day 9 of sponge insertion. On the morning of day 13, 50 µg of gonadotropin‐releasing hormone (GnRH) was injected into each animal. Twenty‐nine hours after GnRH injection, LFA was performed. After removal of cumulus cells, collected oocytes with the first polar body were selected and enucleated for nuclear transfer. Anterior pituitary cells isolated from an adult male Shiba goat were transfected with a DNA fragment containing the enhanced green flourescent protein gene and the puromycin resistance gene. A single donor cell was inserted into the perivitelline space of each enucleated oocyte and fusion was induced with one electric pulse of 20 V for 10 µs. The SCNT goat eggs were cultured in chemically defined medium at 38.5°C in 5% CO2, 5% O2, 90% N2 for 9 days. By LFA, 396 oocytes were collected from a total of 30 females. After removal of cumulus cells, 64% of them extruded the first polar body. The percentage of SCNT goat eggs produced using in vivo matured oocytes which developed to the blastocyst stage (20–21%) was significantly higher (P < 0.05) than that produced with in vitro matured oocytes (3–8%).

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“…Oócitos podem ser obtidos por laparoscopia de fêmeas que não são estimuladas com hormônios [39,65], ou de fêmeas ovinas estimuladas com FSH/ LH, combinado ou não ao uso de eCG, 24 ou 48h antes da aspiração [4]. Também é utilizado FSH em dose única [1] ou em várias aplicações [58], injeção única de eCG, 48h antes da aspiração [62], a qual pode ser associada à administração de anticorpos monoclonais anti-eCG, logo após a aspiração [1,66]. Para fêmeas não estimuladas com hormônios foi relatada a obtenção de 4,6 [39] ou 6,9 [65] oócitos por sessão de aspiração.…”

Section: Origem E Obtenção Dos Oócitosunclassified

Produção in vitro de embriões ovinos

Bernardi

2018

Acta Scientiae. Vet.

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A produção in vitro (PIV) de embriões compreende as etapas de maturação dos oócitos, capacitação dos espermatozóides, fecundação dos oócitos e cultivo dos zigotos até o estádio de mórula ou blastocisto. Embora a execução destas etapas não seja complexa, vários fatores podem interferir no seu sucesso. Se uma das etapas não é realizada corretamente, uma redução do índice de sucesso na etapa seguinte pode ocorrer. Nesta revisão são detalhadas as etapas referentes à PIV de embriões ovinos, sendo enfocados a eficiência de cada uma e os avanços obtidos. Também são enfatizados os aspectos que devem ser melhorados para que as tecnologias associadas à PIV de embriões ovinos tenha uma aplicação mais difundida.

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In vitro development of sheep embryos derived of repeated laparoscopic folliculoaspiration

Cited by 7 publications

References 0 publications

Ovum Pick‐up in Sheep: a Comparison between Different Aspiration Devices for Optimal Oocyte Retrieval

Ovum Pick‐up in Sheep: a Comparison between Different Aspiration Devices for Optimal Oocyte Retrieval

Caprine somatic cell nuclear transfer using in vivo matured oocytes collected by laparoscopic follicular aspiration

Produção in vitro de embriões ovinos

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