In vitro differentiation and plant regeneration of Albizia chinesis (Osb.) Merr

Sinha, Raj; Majumdar, K. C.; Sinha, S. P.

doi:10.1007/s11627-000-0066-7

Cited by 25 publications

(15 citation statements)

References 15 publications

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“…The welldeveloped shoots were regularly removed and transferred to rooting medium. The enhanced multiplication of shoots during subsequent subculturing substantiates the earlier report on Albizia chinensis (Sinha et al 2000), Vitex negundo ( and Holarrhena antidysenterica (Raha and Roy 2001). Thus, the regenerating medium (MS) containing BA (5.0 μM) and NAA (0.5 μM) may be recommended as the best suitable medium for long-term maintenance of regenerative potential of shoot tip explants of V. trifolia.…”

Section: Effect Of Subculturingsupporting

confidence: 85%

Rapid in vitro propagation system through shoot tip cultures of Vitex trifolia L.–an important multipurpose plant of the Pacific traditional Medicine

Ahmed

Anis

2014

Physiol Mol Biol Plants

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A rapid and efficient plant propagation system through shoot tip explants was established in Vitex trifolia L., a medicinally important plant belonging to the family Verbenaceae. Multiple shoots were induced directly on Murashige and Skoog (MS) medium consisting of different cytokinins, 6-benzyladenine (BA), kinetin (Kin) and 2-isopentenyl adenine (2-iP), BA at an optimal concentration of 5.0 μM was most effective in inducing multiple shoots where 90 % explants responded with an average shoot number (4.4 ±0.1) and shoot length (2.0±0.1 cm) after 6 weeks of culture. Inclusion of NAA in the culture medium along with the optimum concentration of BA promoted a higher rate of shoot multiplication and length of the shoot, where 19.2±0.3 well-grown healthy shoots with an average shoot length of 4.4±0.1 cm were obtained on completion of 12 weeks culture period. Ex vitro rooting was achieved best directly in soilrite when basal portion of the shoots were treated with 500 μM indole-3-butyric acid for 15 min which was the most effective in inducing roots, as 95 % of the microshoots produced roots. Plantlets went through a hardening phase in a controlled plant growth chamber, prior to ex-vitro transfer. Micropropagated plants grew well, attained maturity and flowered with 92 % survival rate. The results of this study provide the first report on in vitro plant regeneration of Vitex trifolia L. using shoot tip explants.

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Section: Effect Of Subculturingsupporting

confidence: 85%

Rapid in vitro propagation system through shoot tip cultures of Vitex trifolia L.–an important multipurpose plant of the Pacific traditional Medicine

Ahmed

Anis

2014

Physiol Mol Biol Plants

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“…Kumar et al (1998) described regeneration of plants from leaflet explants in A. procera. Sinha et al (2000) described in vitro differentiation and plant regeneration in A. chinensis. Ramamurthy and Savithramma (2003) described shoot bud regeneration from leaf explants.…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation studies of Albizia amara (Roxb.) Boiv.

Indravathi¹,

Pullaiah²

2013

Afr. J. Plant Sci.

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“…An inhibitory effect of higher concentrations of BA on in vitro shoot proliferation has also been reported earlier in Albizia chinensis (Osbeck) Merr. (Sinha et al 2000), Pterocarpus marsupium (Swynn. ex …”

Section: In Vitro Establishment and Multiplicationmentioning

confidence: 99%

High frequency in vitro regeneration system for conservation of Barleria prionitis L., a threatened medicinal shrub

Soni¹,

Kumari²,

Swarnkar³

2017

Bot Pac

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A rapid and efficient plant propagation system through nodal and embryo cul tures was developed for conservation of threatened shrub Barleria prionitis. Nodal explants exhibited high frequency shoot proliferation on Murashige and Skoog (MS) medium supplemented with 1.0 mg l 1 6 benzylaminopurine (BA) and 0.5 mg l 1 thidiazuron (TDZ). Microshoots were best rooted on half strength MS fortified with 0.5 mg l 1 indole 3 butyric acid (IBA). Maximum conversion (63.6 %) of zygotic embryos into well rooted plantlets was achieved on half strength MS supplemented with 20 mg l 1 sucrose, devoid of any growth re gu lator S. Plantlets with high PSII photochemical efficiency (Fv /Fm ≥ 0.8) were successfully shifted to natural conditions. The overall survival rate during ac cli matization from in vitro growth to field transfer was 81 %. The developed mic ro pro pagation protocol can be successfully used for large scale multiplication and conservation this high value medicinal plant species. Р Е З Ю М ЕСони В., Кумари Р., Шварнкар П.Л. Система интенсивной ре ге не ра ции in vitro для сохранения Barleria prionitis L., редкого ле карст венного кус тар ника. Разработана быстрая и эффективная система размножения рас те ний через меристемные и эмбриональные культуры для сохранения уг ро жаемого кустарникового вида Barleria prionitis. Меристемные экс плантаты де монстрировали интенсивную пролиферацию побегов на среде Mura shi ge и Skoog (MS), дополненную 1,0 мг/л 6 бензиламинопурином (BA) и 0,5 мг/л ти ди азуроном (TDZ). Микропобеги лучше всего укоренялись на MS, обогащенной 0,5 мг/л индол 3 масляной кислотой (IBA). Максимальное пре об разо ва ние (63,6 %) зиготных эмбрионов в укоренившиеся растения бы ло достигнуто на MS, до пол ненной 20 мг/л сахарозы, лишенной ка ко го ли бо регулятора роста S. Растеньица с высокой фотохимической эф фек тив ностью PSII (Fv / Fm ≥ 0,8) были успешно перенесены в ес тест вен ную среду. Общая выживаемость при акклиматизации от роста in vitro до пе ре но са в естественную среду составляла 81 %. Разработанный про то кол мик ро размножения может быть успешно использован для круп но мас штаб но го размножения и сохранения этих высокоценных лекарственных растений.

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In vitro differentiation and plant regeneration of Albizia chinesis (Osb.) Merr

Cited by 25 publications

References 15 publications

Rapid in vitro propagation system through shoot tip cultures of Vitex trifolia L.–an important multipurpose plant of the Pacific traditional Medicine

Rapid in vitro propagation system through shoot tip cultures of Vitex trifolia L.–an important multipurpose plant of the Pacific traditional Medicine

In vitro propagation studies of Albizia amara (Roxb.) Boiv.

High frequency in vitro regeneration system for conservation of Barleria prionitis L., a threatened medicinal shrub

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