In vitro differentiation and plant regeneration from root and other explants of juvenile origin in pea (Pisum sativum L.)

Sharma, Shikha; Gambhir, Geetika; Srivastava, D. K.

doi:10.18805/lr.v0iof.9097

Cited by 4 publications

(5 citation statements)

References 15 publications

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“…As the MS basal medium was carefully removed from the plants, no contamination was detected in the aquarium environment. Similarly, the acclimatization of plants propagated in vitro to external conditions have been reported in Alternanthera sessilis (L.) (Gnanaraj et al, 2011), R. rotundifolia (Dogan, 2017), Pisum sativum L. (Sharma et al, 2017), Monochasma savatieri Franch ex Maxim (Zhang et al, 2017) and Enicostema axillare (Sasidharan and Jayachitra, 2017). Consequently, multiple shoot formation from shoot and nodule explants of P. erectus was successfully achieved in culture media containing different TDZ and 2,4-D combinations.…”

Section: Resultsmentioning

confidence: 72%

“…The correct choice of explant variety in tissue culture studies has an important effect on the success of the study. For this reason, researchers have experimented with different explant varieties such as shoot tip (Sasidharan and Jayachitra, 2017;Bourrain, 2018), node (Dobranszki et al, 2017;Irshad et al, 2018), internode (Thul and Kukreja, 2010;Zhang et al, 2017), leaf (Mahindrakar et al, 2018) and root (Sharma et al, 2017). In the present study, shoot tip and nodal explants of P. erectus were cultured in MS medium containing 0.10-0.80 mg/L TDZ + 0.10 mg/L 2,4-D for rapid and multiple production under in vitro conditions.…”

Section: Resultsmentioning

confidence: 99%

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Sucul bir bitki olan Pogostemon erectus (Dalzell) Kuntze’un In Vitro hızlı çoğaltımı

Doğan

2019

Anatolian Journal of Botany

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The aim of this study is to investigate the multiple and rapid production of Pogostemon erectus (Dalzell) Kuntze by tissue culture techniques. The shoot tip and nodal explants of P. erectus were isolated and then cultured in Murashige and Skoog (1962) (MS) nutrient media containing thidiazuron (TDZ) and 2,4-Dichlorophenoxyacetic acid (2,4-D) in different combinations. The first shoot formation on the explants was observed on day 15. The number of shoots per explant ranged from 2.85 to 29.39 in the shoot tip explants and from 2.72 to 32.24 in the nodal explants. The maximum number of shoots per explant was obtained in the MS medium containing 0.20 mg/L TDZ + 0.10 mg/L 2,4-D for shoot tip explant (29.39) and in the MS medium containing 0.10 mg/L TDZ + 0.10 mg/L 2,4-D for nodal explant (32.24). The longest shoots in the shoot tip (1.76 cm) and nodal (1.64 cm) explants were determined in MS medium supplemented with 0.10 mg/L TDZ + 0.10 mg/L 2,4-D. Regenerated shoots were rooted in MS medium supplemented with 0.25 mg/L indol-3yl acetic acid (IAA), and then acclimatized to aquarium conditions.

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Section: Resultsmentioning

confidence: 72%

Section: Resultsmentioning

confidence: 99%

Sucul bir bitki olan Pogostemon erectus (Dalzell) Kuntze’un In Vitro hızlı çoğaltımı

Doğan

2019

Anatolian Journal of Botany

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“…Ellethy et al (2011) stated that riboflavin enhanced that root and shoot fresh biomass by foliar application in Pelargonium graveolens and Hibiscus sabdariffa. It was also noted by noted Sharma et al (2017) that 1.86 mg/L NAA was useful for shoot development in pea. Chl.…”

Section: Yield Attributesmentioning

confidence: 84%

Efficacy of IAA, GA3 and Riboflavin for Morpho-biochemical and Yield Attributes of Fenugreek (Trigonella foenum-graecum L.) in Pakistan

Tufail¹,

Hussain²,

Iqbal³

et al. 2020

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Background: Fenugreek is an important winter leafy vegetable in Pakistan with high nutritional values. Its seeds are used as spices and whole plant is used in many home remedies and medicine throughout the world. Its foliage and seed productivity is much low in the region. Plant growth regulators (PGRs) and vitamins (riboflavin) can be useful for the enhancement of plant productivity. Methods: Treatments of indole acetic acid (IAA), gibberellic acid (GA3) and riboflavin (50 mmol L-1 each) were applied as foliar spray after 14 days of sowing on fenugreek variety Kasuri Methi. Experiment was arranged in a completely randomized design (CRD) with six replicates. Result: Result showed that GA3 and IAA has significantly enhanced the root and shoot development, foliage growth and ions concentrations, peroxidase (POD) and catalases (CAT) activities. PGRs also increased the pod and seed yield of fenugreek. Riboflavin did not show any significant effect on fenugreek except increased the antioxidant activities at seedling stage. GA3 was more effective as compared to IAA for the enhancement biomass production, yield and biochemical attributes of fenugreek. It was determined that IAA and GA3 can be used to enhance the foliage biomass production and yield but GA3 is superior over IAA in fenugreek. These outcomes can be useful for economic benefits with high production of this leafy vegetable.

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“…Recently, many tissue culturists have focused on ex vitro rhizogenesis in microshoots because ex vitro rooted plantlets produce a better developed root system compared with those of in vitro raised plantlets [ 129 , 292 , 293 ]. The ex-vitro rooting strategy is practical and cost-effective, requiring less time, less labor, fewer chemicals, and only minimal equipment compared with the requirements of in vitro rooting practice.…”

Section: Propagation Of Pterocarpus Marsupiummentioning

confidence: 99%

Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

Ahmad

Anis

et al. 2022

Plants

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Trees are vital resources for economic, environmental, and industrial growth, supporting human life directly or indirectly through a wide variety of therapeutic compounds, commodities, and ecological services. Pterocarpus marsupium Roxb. (Fabaceae) is one of the most valuable multipurpose forest trees in India and Sri Lanka, as it is cultivated for quality wood as well as pharmaceutically bioactive compounds, especially from the stem bark and heartwood. However, propagation of the tree in natural conditions is difficult due to the low percentage of seed germination coupled with overexploitation of this species for its excellent multipurpose properties. This overexploitation has ultimately led to the inclusion of P. marsupium on the list of endangered plant species. However, recent developments in plant biotechnology may offer a solution to the overuse of such valuable species if such advances are accompanied by technology transfer in the developing world. Specifically, techniques in micropropagation, genetic manipulation, DNA barcoding, drug extraction, delivery, and targeting as well as standardization, are of substantial concern. To date, there are no comprehensive and detailed reviews of P. marsupium in terms of biotechnological research developments, specifically pharmacognosy, pharmacology, tissue culture, authentication of genuine species, and basic gene transfer studies. Thus, the present review attempts to present a comprehensive overview of the biotechnological studies centered on this species and some of the recent novel approaches for its genetic improvement.

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In vitro differentiation and plant regeneration from root and other explants of juvenile origin in pea (Pisum sativum L.)

Cited by 4 publications

References 15 publications

Sucul bir bitki olan Pogostemon erectus (Dalzell) Kuntze’un In Vitro hızlı çoğaltımı

Sucul bir bitki olan Pogostemon erectus (Dalzell) Kuntze’un In Vitro hızlı çoğaltımı

Efficacy of IAA, GA3 and Riboflavin for Morpho-biochemical and Yield Attributes of Fenugreek (Trigonella foenum-graecum L.) in Pakistan

Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

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