In vitro-digested milk proteins: Evaluation of angiotensin-1-converting enzyme inhibitory and antioxidant activities, peptidomic profile, and mucin gene expression in HT29-MTX cells

Giromini, Carlotta; Lovegrove, Julie A.; Givens, D.I.; Rebucci, R.; Pinotti, L.; Maffioli, Elisa; Tedeschi, Gioacchino; Sundaram, Tamil Selvi; Baldi, Alfonso

doi:10.3168/jds.2019-16833

Cited by 23 publications

(19 citation statements)

References 46 publications

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“…Previously, the significance of cell models and cell-based assays available for screening antioxidants was described [ 25 ]. In particular, intestinal cell models are widely utilized for the validation of bioactive compounds in food and feed analysis [ 15 , 26 , 27 , 28 ]. Earlier, the anti-inflammatory and anti-necroptosis activity of ω3-PUFAs were demonstrated in normal and cancer enterocyte models [ 12 , 13 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

Omega-3 Polyunsaturated Fatty Acids Counteract Inflammatory and Oxidative Damage of Non-Transformed Porcine Enterocytes

Sundaram

Giromini

Rebucci

et al. 2020

Animals

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Marine and plant-based omega-3 polyunsaturated fatty acids (ω-3 PUFAs) are widely added to animal diets to promote growth and immunity. We tested the hypothesis that eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and their 1:2 combination could counteract acute or long-term damage of lipopolysaccharides (LPS), dextran sodium sulphate (DSS) and hydrogen peroxide (H2O2) in Intestinal Porcine Epithelial Cell line-J2 (IPEC-J2). The results showed that 24 h treatment with EPA or DHA exhibited proliferative effects in IPEC-J2 cells at low to moderate concentrations (6.25–50 μM) (p < 0.05). Further, 24 h pretreatment with individual DHA (3.3 µM), EPA (6.7 µM) or as DHA:EPA (1:2; 10 µM) combination increased the mitochondrial activity or cell membrane integrity post-LPS (24 h), DSS (24 h) and H2O2 (1 h) challenge (p < 0.05). Additionally, DHA:EPA (1:2, 10 µM) combination decreased the apoptotic caspase-3/7 activity around twofold after 24 h LPS and DSS challenge (p < 0.05). Our study confirms the proliferative and cytoprotective properties of EPA and DHA in IPEC-J2 cells. Increased intracellular mitochondrial activity and cell membrane integrity by ω-3 PUFAs can play a role in preventing enterocyte apoptosis during acute or chronic inflammatory and oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Omega-3 Polyunsaturated Fatty Acids Counteract Inflammatory and Oxidative Damage of Non-Transformed Porcine Enterocytes

Sundaram

Giromini

Rebucci

et al. 2020

Animals

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“…The digestion procedure was performed twice (2 biological replicates). Whey protein (90%) was included as a reference sample for stability tests in all digestions performed, as previously indicated in Giromini et al [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…Due to such restrictions, alternatives to antibiotics and alternatives to zinc oxide are urgently needed to guarantee animal production in line with health principles [ 11 , 12 ]. In this scenario, plant extracts can represent a valuable alternative as a natural source of functional compounds, such as polyphenolic compounds [ 13 , 14 , 15 , 16 , 17 ]. Among polyphenols, tannins derived from plant extracts are the largest class which can be classified into hydrolysable (HTs) or condensed (CTs) subgroups [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Dietary Administration of Chestnut and Quebracho Tannins on Growth, Serum Metabolites and Fecal Parameters of Weaned Piglets

Caprarulo

Hejna

Giromini

et al. 2020

Animals

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In pig livestock, alternatives to in-feed antibiotics are needed to control enteric infections. Plant extracts such as tannins can represent an alternative as a natural source of functional compounds. The aim of this study was to evaluate the in vitro digestibility and in vivo effects of oral supplementation of combined chestnut (Ch) and quebracho (Qu) tannins in order to establish if they can induce a positive effect on weaned piglets’ performance, metabolic status and fecal parameters. In vitro digestibility (dry matter, DM) of diets was calculated using a multi-step enzymatic technique. In vitro digested diet samples were further tested on an intestinal porcine enterocyte cell line (IPEC-J2). Weaned piglets (n = 120; 28 ± 2 day old) were randomly allotted to two groups (12 pens in total with 10 pigs per pen): control (Ctrl) and treatment (Ch/Qu). After one week of adaptation (day 0), 35-day-old piglets in the Ctrl group were fed a Ctrl diet and the Ch/Qu group were fed with 1.25% Ch/Qu for 40 days. Body weight and feed intake per pen were recorded weekly. At day 40, blood and fecal samples were collected. Principal metabolic parameters were evaluated from blood samples by enzymatic colorimetric analysis. Total phenolic compounds, urea, and ammonia in feces were analyzed (Megazyme International, Bray, Ireland). In vitro digestibility and cell viability assays showed that the inclusion of 1.25% Ch/Qu slightly reduced diet digestibility compared with the Ctrl diet, while intestinal cell viability was not altered with low concentrations of Ch/Qu digesta compared with Ctrl. In vivo results did not show any adverse effects of Ch/Qu on feed intake and growth performance, confirming that dietary inclusion of Ch/Qu at a concentration of 1.25% did not impair animal performance. The decreased diet DM digestibility in the Ch/Qu diet may cause increased serum concentration of albumin (Ctrl: 19.30 ± 0.88; Ch/Qu: 23.05 ± 0.88) and albumin/globulin ratio (Ctrl: 0.58 ± 0.04; Ch/Qu: 0.82 ± 0.04), but decreased creatinine (Ctrl: 78.92 ± 4.18; Ch/Qu: 54.82 ± 4.18) and urea (Ctrl: 2.18 ± 0.19; Ch/Qu: 0.95 ± 0.19) compared with Ctrl. Pigs in the Ch/Qu group contained higher (p < 0.05) concentrations of fecal phenolic compounds and nitrogen than the Ctrl group, while fecal ammonia and urea were not affected by tannins. In conclusion, Ch/Qu tannin supplementation did not influence growth performance. Although lower digestibility was observed in the diet supplemented with Ch/Qu tannins, Ch/Qu supplementation did not show any adverse effect on intestinal epithelial cell viability.

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“…MET-ADS in vitro digestion was performed according to the method of Minekus et al [27] and further adapted by Giromini et al [28] and Miralles et al [29]. Briefly, 20 g of MET-ADS were mixed with 150 mL of distilled H 2 O and maintained on an orbital shaker at 150 rpm for 5 min.…”

Section: Met-ads In Vitro Digestion Simulationmentioning

confidence: 99%

Natural Clays as Potential Amino Acids Carriers for Animal Nutrition Application

et al. 2021

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This study has the main purpose of testing natural carriers as more sustainable alternatives to polymeric coating. New clay-based hybrid organic–inorganic materials have been prepared. An innovative procedure for the intercalation of the bioactive molecule is proposed. The synthesis process implies the use of mild operating conditions and does not require the use of organic reactants or solvents. Moreover, the use of natural carriers, and milder environmentally friendly process conditions, fulfill the requirement of a mostly safe, greener production. The capture and the release properties of the final material mainly depend on the carrier morphology. Large zeolite content in the mineral clays can favor the capture of very large methionine loadings, but due to the trapping mechanism, typical of these structures, zeolites prevent a satisfactory release in mild condition and physiological condition. The Freundlich adsorption model has been found to be able to predict the capture behavior. Once the proper carrier is selected, the preparation procedure guarantees the protection of the amino acid in the right form. Neither the structure of the inorganic matrix nor the amino acid structure was changed in the preparation process. Methionine was detected in its zwitterionic form, showing the main IR bands only slightly affected by the interaction. Further studies on methionine bioaccessibility and intestinal absorption from methionine–Adsorbene (MET–ADS) are required to optimize the application of natural clay in in-field animal nutrition.

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In vitro-digested milk proteins: Evaluation of angiotensin-1-converting enzyme inhibitory and antioxidant activities, peptidomic profile, and mucin gene expression in HT29-MTX cells

Cited by 23 publications

References 46 publications

Omega-3 Polyunsaturated Fatty Acids Counteract Inflammatory and Oxidative Damage of Non-Transformed Porcine Enterocytes

Omega-3 Polyunsaturated Fatty Acids Counteract Inflammatory and Oxidative Damage of Non-Transformed Porcine Enterocytes

Evaluation of Dietary Administration of Chestnut and Quebracho Tannins on Growth, Serum Metabolites and Fecal Parameters of Weaned Piglets

Natural Clays as Potential Amino Acids Carriers for Animal Nutrition Application

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