In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota

Wu, Ding‐Tao; Feng, Kang-Lin; Li, Fen; Hu, Yichen; Wang, Shengpeng; Gan, Ren‐You; Zou, Liang

doi:10.1016/j.crfs.2022.04.004

Cited by 20 publications

(7 citation statements)

References 41 publications

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“…Figure 4 A shows the HPSEC-RID chromatograms of P-W and P-DM. Results showed that both P-W and P-DM possessed only one symmetric polysaccharide fraction, indicating that the combined use of graded alcohol precipitation and membrane separation could effectively promote the purity of polysaccharides extracted from plants [ 37 ]. As displayed in Table 3 , the molecular weight of P-W (2.541 × 10 5 Da) was much higher than that of P-DM (1.575 × 10 5 Da).…”

Section: Resultsmentioning

confidence: 99%

Microwave-Assisted Deep Eutectic Solvent Extraction, Structural Characteristics, and Biological Functions of Polysaccharides from Sweet Tea (Lithocarpus litseifolius) Leaves

Guo

et al. 2022

Antioxidants

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The leaf of sweet tea (Lithocarpus litseifolius) is widely used as an edible and medicinal plant in China, which is rich in bioactive polysaccharides. In order to explore and promote the application of sweet tea polysaccharides in the functional food industry, the microwave-assisted deep eutectic solvent extraction (MDAE) of polysaccharides from sweet tea leaves was optimized, and the structural properties and biological functions of sweet tea polysaccharides prepared by MDAE (P-DM) were investigated and compared with that of hot water extraction (P-W). The maximum yield (4.16% ± 0.09%, w/w) of P-DM was obtained under the optimal extraction conditions (extraction time of 11.0 min, extraction power of 576.0 W, water content in deep eutectic solvent of 21.0%, and liquid–solid ratio of 29.0 mL/g). Additionally, P-DM and P-W possessed similar constituent monosaccharides and glycosidic bonds, and the homogalacturonan (HG) and arabinogalactan (AG) might exist in both P-DM and P-W. Notably, the lower molecular weight, higher content of total uronic acids, and higher content of conjugated polyphenols were observed in P-DW compared to P-W, which might contribute to its much stronger in vitro antioxidant, anti-diabetic, antiglycation, and prebiotic effects. Besides, both P-DW and P-W exhibited remarkable in vitro immunostimulatory effects. The findings from the present study indicate that the MDAE has good potential to be used for efficient extraction of bioactive polysaccharides from sweet tea leaves and P-DM can be developed as functional food ingredients in the food industry.

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Section: Resultsmentioning

confidence: 99%

Microwave-Assisted Deep Eutectic Solvent Extraction, Structural Characteristics, and Biological Functions of Polysaccharides from Sweet Tea (Lithocarpus litseifolius) Leaves

Guo

et al. 2022

Antioxidants

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“…Moreover, NPSYP and HPSYP promoted increases in Bifidobacterium by 28.32% and 23.5%. Bifidobacterium has been identified as one of the important probiotics in the human gut, and can protect the host from infection by enteric pathogens by producing acetate [ 44 ]. NPBY and HPBYP promoted increases in Prevotella _9 by 26.33% and 23.98%.…”

Section: Resultsmentioning

confidence: 99%

Domestic Cooking Affects the Prebiotic Performances of Chinese Yam

Gong

Liu

et al. 2022

Foods

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The appropriate domestic cooking methods can retain and protect the biological properties of foods well. Thus, the objective of this study was to unravel the effect of different cooking methods on the microbiota modulatory properties of yam and their non-starch polysaccharides by an in vitro simulated digestion and fermentation model. The results showed that different cooking processes led to different changes in polysaccharide content. The polysaccharide content of yam increased by 21.3~108.2% or decreased by 12.0% compared with that of raw yam. Additionally, the soluble polysaccharides contents in all cooked yam samples significantly increased by 16.85~119.97% after in vitro digestion. The regulation of whole-yam digesta on gut microbiota was partly related with yam polysaccharides. Both yam and yam polysaccharide fermentation appeared to promote beneficial bacteria, such as Bifidobacteria, Bacteroides spp. and Megasphaera and suppressed bacterial pathogens such as Ruminococcusforques and Escherichia-Shigella. Household cooking significantly influenced the prebiotic performances of yam and yam polysaccharides by changing the heat-sensitive microbial substrates and their physiology properties. According to our results, normal-pressure steaming and normal-pressure boiling processes can retain the microbiota modulatory effects of Chinese yam.

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“…LRP demonstrated the capacity to modulate intestinal microbiota by promoting Bifidobacterium ’s growth and altering the ratio of Firmicutes / Bacteroidetes , thereby promoting the generation of SCFAs (butyric, propionic, and acetic) ( 61 ). Besides, leaf LPs (LLPI) selectively improved the abundance of beneficial microbes, including Megamonas, Bacteroides , Bifidobacterium , and Collinsella , and effectively promoted the production of SCFAs ( 100 ). These findings underscore the potential of LPs to improve the intestinal micro-environment and highlight their prebiotic properties.…”

Section: Biological Activitiesmentioning

confidence: 99%

Bioactive polysaccharides from lotus as potent food supplements: a review of their preparation, structures, biological features and application prospects

et al. 2023

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Lotus is a famous plant of the food and medicine continuum for millennia, which possesses unique nutritional and medicinal values. Polysaccharides are the main bioactive component of lotus and have been widely used as health nutritional supplements and therapeutic agents. However, the industrial production and application of lotus polysaccharides (LPs) are hindered by the lack of a deeper understanding of the structure–activity relationship (SAR), structural modification, applications, and safety of LPs. This review comprehensively comments on the extraction and purification methods and structural characteristics of LPs. The SARs, bioactivities, and mechanisms involved are further evaluated. The potential application and safety issues of LPs are discussed. This review provides valuable updated information and inspires deeper insights for the large scale development and application of LPs.

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In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota

Cited by 20 publications

References 41 publications

Microwave-Assisted Deep Eutectic Solvent Extraction, Structural Characteristics, and Biological Functions of Polysaccharides from Sweet Tea (Lithocarpus litseifolius) Leaves

Microwave-Assisted Deep Eutectic Solvent Extraction, Structural Characteristics, and Biological Functions of Polysaccharides from Sweet Tea (Lithocarpus litseifolius) Leaves

Domestic Cooking Affects the Prebiotic Performances of Chinese Yam

Bioactive polysaccharides from lotus as potent food supplements: a review of their preparation, structures, biological features and application prospects

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