In Vitro Echogenicity Characterization of Poly[lactide-coglycolide] (PLGA) Microparticles and Preliminary In Vivo Ultrasound Enhancement Study for Ultrasound Contrast Agent Application

Lavisse, S.; Paci, Angélo; Rouffiac, Valérie; Adotevi, Cecile; Opolon, Paule; Péronneau, P.; Bourget, Philippe; Roche, Alain; Perricaudet, Michel; Fattal, Elias; Lassau, Nathalie

doi:10.1097/01.rli.0000170818.03210.ee

Cited by 16 publications

(10 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This development of NPs as imaging-contrast agents may also offer enhanced sensitivity and specificity for in vivo tumor imaging. For example, an in vivo study demonstrated that PLGA microparticles induced a significant enhancement of 47% intratumoral vessels detected after injection [84]. Goldberg et al developed PLGA perfluorocarbon-filled microparticles and injected them in rabbits with VX2 tumors.…”

Section: Cancersmentioning

confidence: 99%

Current advances in research and clinical applications of PLGA-based nanotechnology

Lü

Wang

Marín-Müller

et al. 2009

Expert Review of Molecular Diagnostics

776

333

View full text Add to dashboard Cite

Co-polymer poly(lactic-co-glycolic acid) (PLGA) nanotechnology has been developed for many years and has been approved by the US FDA for the use of drug delivery, diagnostics and other applications of clinical and basic science research, including cardiovascular disease, cancer, vaccine and tissue engineering. This article presents the more recent successes of applying PLGA-based nanotechnologies and tools in these medicine-related applications. It focuses on the possible mechanisms, diagnosis and treatment effects of PLGA preparations and devices. This updated

show abstract

Section: Cancersmentioning

confidence: 99%

Current advances in research and clinical applications of PLGA-based nanotechnology

Lü

Wang

Marín-Müller

et al. 2009

Expert Review of Molecular Diagnostics

776

333

View full text Add to dashboard Cite

show abstract

“…Various polymeric microbubbles have been investigated for ultrasonic imaging (Forsberg et al 2004; Lavisse et al 2005; Ketterling et al 2007; Grishenkov et al 2009a; Grishenkov et al 2009b; Lu et al 2009; Yang et al 2009; Sciallero et al 2012) and targeted drug delivery/therapeutics (Wheatley et al 2007; Jie et al 2008; Lu et al 2009; Sirsi et al 2009) (see the review by Xiong et al (Xiong et al 2011)). Specifically, PLA as well as PLGA—a block copolymer with varying lactic to co-glycolic acid ratios—has been investigated as an encapsulating material for contrast microbubbles.…”

Section: Introductionmentioning

confidence: 99%

Determination of the Interfacial Rheological Properties of a Poly(DL-lactic acid)-Encapsulated Contrast Agent Using In Vitro Attenuation and Scattering

Paul

Russakow

Rodgers

et al. 2013

Ultrasound in Medicine & Biology

View full text Add to dashboard Cite

The stabilizing encapsulation of a microbubble based ultrasound contrast agent (UCA) critically affects its acoustic properties. Polymers, which behave differently from commonly used materials—e.g. lipids or proteins—for the monolayer encapsulation, hold potential for better stability and control over encapsulation properties. Air-filled microbubbles coated with Poly (D, L-lactide) (PLA) are characterized here using in vitro acoustic experiments and several models of encapsulation. The interfacial rheological properties of the encapsulation are determined according to each of these models using attenuation of ultrasound through a suspension of these microbubbles. Then the model predictions are compared with scattered nonlinear—sub- and second harmonic—responses. For this microbubble population (average diameter 1.9 μm), the peak in attenuation measurement indicates a weighted average resonance frequency of 2.5–3 MHz, which, in contrast to other encapsulated microbubbles, is lower than the resonance frequency of a free bubble of similar size (diameter 1.9 μm). This apparently contradictory result stems from the extremely low surface dilatational elasticity (around 0.01–0.07 N/m) and the reduced surface tension of the PLA encapsulation as well as the polydispersity of the bubble population. All models considered here are shown to behave similarly even in the nonlinear regime because of the low value of the surface dilatational elasticity. Pressure dependent scattering measurements at two different excitation frequencies (2.25 and 3 MHz) show strongly non-linear behavior with 25–30 dB and 5–20 dB enhancements in fundamental and second-harmonic responses respectively for a concentration of 1.33 μg/mL of suspension. Subharmonic responses are registered above a relatively low generation threshold of 100–150 kPa with up to 20 dB enhancement beyond that pressure. Numerical predictions from all models show good agreement with the experimentally measured fundamental response, but not with the second harmonic response. The characteristic features of subharmonic response and the steady response beyond the threshold are matched well by model predictions. However, prediction of the threshold value depends on property values and the size distribution. The variation in size distribution from sample to sample leads to variation in estimated encapsulation property values—the lowest estimated value of surface dilatational viscosity better predicts the subharmonic threshold.

show abstract

“…In vivo contrast-enhanced power Doppler ultrasonography evaluation on melanoma grafted mice showed 50% enhancement of detection of intratumoral vascularization after injection of 3-mm poly(lactide-coglycolide) microparticles. No toxicity was histologically observed (41).…”

Section: Contrast Agents Molecular Probes and Tracersmentioning

confidence: 99%

“…Ultrasound techniques have also been shown to be very useful in murine cancer research (38)(39)(40)(41)(42)(43)(44). Highfrequency UBM has been used for the quantitative analysis of early tumor growth in mice bearing melanoma xenografts (38).…”

Section: Ultrasoundmentioning

confidence: 99%

“…error between ultrasonic and histological evaluations of maximum cross-sectional area lower than 20%. Several ultrasound contrast agents have been reported to improve the detection of tumor vasculature (41,42) (see also under Contrast agents, molecular probes and tracers). Three-dimensional (3D) ultrasound microimaging techniques have also been used to study transgenic prostate cancer models in mice (43,44).…”

Section: Ultrasoundmentioning

confidence: 99%

See 1 more Smart Citation

In Vivo mouse imaging and spectroscopy in drug discovery

et al. 2007

View full text Add to dashboard Cite

Imaging modalities such as micro-computed tomography (micro-CT), micro-positron emission tomography (micro-PET), high-resolution MRI, optical imaging, and high-resolution ultrasound have become invaluable tools in preclinical pharmaceutical research. They can be used to non-invasively investigate, in vivo, rodent biology and metabolism, disease models, and pharmacokinetics and pharmacodynamics of drugs. The advantages and limitations of each approach usually determine its application, and therefore a small-rodent imaging laboratory in a pharmaceutical environment should ideally provide access to several techniques. In this paper we aim to illustrate how these techniques may be used to obtain meaningful information for the phenotyping of transgenic mice and for the analysis of compounds in murine models of disease.

show abstract

In Vitro Echogenicity Characterization of Poly[lactide-coglycolide] (PLGA) Microparticles and Preliminary In Vivo Ultrasound Enhancement Study for Ultrasound Contrast Agent Application

Abstract: The 3-microm PLGA microparticles provided significant enhancement in vitro and in vivo without any toxicity.

Cited by 16 publications

References 39 publications

Current advances in research and clinical applications of PLGA-based nanotechnology

Current advances in research and clinical applications of PLGA-based nanotechnology

Determination of the Interfacial Rheological Properties of a Poly(DL-lactic acid)-Encapsulated Contrast Agent Using In Vitro Attenuation and Scattering

In Vivo mouse imaging and spectroscopy in drug discovery

Contact Info

Product

Resources

About