In vitro Effect of Alpha-Interferon on Mononuclear Cells of Normal and Autoimmune Patients

Peripheral blood mitogen-induced cellular cytotoxicity (MICC) was studied in 13 untreated patients with active rheumatoid arthritis (RA; group A) and in 5 RA patients with inactive disease (group B), using phytohemagglutinin (PHA) as stimulating agent and K562 cells as target cells in the chromium-51 release assay. MICC was found to be significantly reduced in the patients of group A compared with normal subjects (P < 0.01) and the patients of group B (P < 0.05). No differences were noted in MICC between group B patients and normal subjects. A statistically significant negative correlation was found between values of patients' MICC and serum C-reactive protein levels (r = -0.685, P < 0.01). Furthermore, patients' MICC correlated well with patients' peripheral blood natural killer cell activity (P < 0.02), as well as with the absolute number of circulating CD8+ cells. No correlation was found between MICC and duration of disease, erythrocyte sedimentation rate, serum alpha 2-globulins, or the titre of serum rheumatoid factor in the patients studied. We concluded that defective MICC in untreated patients with active RA is probably due to the diminution of the number of CD8+ cells, although a qualitative defect of these cells cannot be excluded.

show abstract

Defective mitogen-induced cellular cytotoxicity in untreated patients with active rheumatoid arthritis

Vaiopoulos

Kyriakou

Papadaki

et al. 1994

Rheumatol Int

View full text Add to dashboard Cite

show abstract

Biochemical and immunological responses of hairy cell leukemia patients to interferonβ

Liberati¹,

Horisberger

Schippa³

et al. 1991

Cancer Immunol Immunother

View full text Add to dashboard Cite

Ten hairy-cell leukemia patients were treated with interferon beta (IFN-beta) at a dose rate of 2 x 10(6) IU/m2 x 5 days for 4 weeks (induction therapy) and, thereafter, at the same dose three times a week for 11 months (maintenance therapy). The effect of this treatment on serum neopterin, beta 2-microglobulin, (2'-5')oligoadenylate [(2'-5')An] levels, intracellular (2'-5')An values and human Mx protein synthesis was analysed. There were significant rises in serum neopterin and (2'-5')An levels during both induction and maintenance, whereas beta 2-microglobulin levels rose only during induction. Rises in intracellular (2'-5')An were documented mainly during induction, but they were not significantly higher than pretherapy values. IFN beta provoked an increase in human Mx protein synthesis over the entire induction-maintenance period, but was only significantly higher than baseline during induction. All markers proved useful for monitoring the effects of IFN beta dose schedules, but were not predictive of clinical outcome. Natural killer activity and IFN gamma production, which were initially defective, followed a different trend from that of the other factors studied, in that increases were documented only late in the course of therapy when the disease was already in remission.

show abstract

Double-Blind Randomized Phase I Study on the Clinical Tolerance and Biological Effects of Natural and Recombinant Interferon-β

Liberati

Horisberger²,

Palmisano³

et al. 1992

Journal of Interferon Research

View full text Add to dashboard Cite

The clinical tolerance of and the effects recombinant human interferon-beta (rHuIFN-beta) obtained from mammalian cells (Chinese hamster ovary cells) exerts on 2',5'-oligoadenyl (2-5A) synthetase activity, human-Mx protein, neopterin, beta 2-microglobulin, interleukin-1 (IL-1) alpha and beta synthesis were compared to those of natural IFN-beta in 12 healthy volunteers. Each subject received a single i.m. injection of 6 x 10(6) IU rHuIFN-beta and natural IFN-beta according to a randomized double-blind cross-over study design. Both were well tolerated and provoked similar changes in clinical indices. Moreover, rHuIFN-beta and natural IFN-beta induced significant and similar increases in 2'-5' adenylates, human Mx protein, and neopterin levels, but neither modulated beta 2-microglobulin, IL-1 alpha or beta synthesis. The sum of these findings indicates that rHuIFN-beta and natural IFN-beta are biologically equivalent. In view of these results, we are of the opinion that these two types of IFN are probably also therapeutically equivalent and, in consequence, that trials to evaluate the response of viral and neoplastic disease patients to rHuIFN-beta are fully justified.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

In vitro Effect of Alpha-Interferon on Mononuclear Cells of Normal and Autoimmune Patients

Cited by 3 publications

References 14 publications

Defective mitogen-induced cellular cytotoxicity in untreated patients with active rheumatoid arthritis

Defective mitogen-induced cellular cytotoxicity in untreated patients with active rheumatoid arthritis

Biochemical and immunological responses of hairy cell leukemia patients to interferonβ

Double-Blind Randomized Phase I Study on the Clinical Tolerance and Biological Effects of Natural and Recombinant Interferon-β

Contact Info

Product

Resources

About