In Vitro Effectiveness of Jamu Ternak Against Paramphistomiasis

Abstract

The prevalence of cattles trematodosis in Bali and Nusa Tenggara at 2016 was 37.21%. Jamu ternak, an appetite enhancer assumed have a potential antiparamphistomiasis effect, but there still a lack of research has been done. This research aim was to evaluate the antiparamphistomiasis activity of jamu ternak. Jamu ternak were prepared and further characterized in terms of organoleptic, specific gravity, pH, and phytochemical screening. Wholemount preparations of Paramphistomum sp. stained with Clitoria ternatea were identified under a microscope. The adult Paramphistomum sp. were divided into 5 groups consist of 0.9% w/v NaCl, Albendazole 10%, and jamu ternak (25, 50, and 100% v/v). The percentage of flukes mortality and motility score were recorded every 15 minutes for 7 hours of incubation. Time of death, relative motility, and flukes survival index values were calculated and statistically analyzed using SPSS version 28 software. Jamu ternak colour is yellow with spicy taste, aromatic odor, specific gravity value of 1,018 and pH of 3,75. Jamu ternak contain secondary metabolites except tannins. All of groups achieved 100% mortality. The average mortality time of flukes in negative control, positive control, jamu ternak (25%, 50% and 100% v/v), respectively were 398.33; 135; 101.67; 128.33; and 108.33 minutes. The relative motility values were 95.24; 19.62; 15.71; 19.48; and 15.48 %. The survival index values were 82.9; 36.95; 27.38; 35.86; and 29.52%. Jamu ternak 25% v/v considered as the effective antiparamphistomiasis dose with the average time of death, relative motility, and survival index value higher than Albendazole 10% w/v (p<0.05).