In vitro effects of the activity of novel platinum (II) complex in canine and human cell lines

Henklewska, Marta; Pawlak, Aleksandra; Kutkowska, Justyna; Pruchnik, Hanna; Rapak, Andrzej; Obmińska-Mrukowicz, Bożena

doi:10.1111/vco.12511

Cited by 8 publications

(4 citation statements)

References 52 publications

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“…The cell proliferation was investigated using the standard MTT test (Sigma-Aldrich, Germany). Briefly, 2 × 10 3 cells were treated with He-DBD for a defined time (0-360 s) and then seeded onto the 96-flat bottom well plates in triplicates [56]. The test was carried out for up to 7 days.…”

Section: Proliferation Assaymentioning

confidence: 99%

Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature

Dzimitrowicz

Bielawska‐Pohl

Jamróz

et al. 2019

Plasma Chem Plasma Process

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Skin injury leading to chronic wounds is of high interest due to the increasing number of patients suffering from this symptom. Proliferation, migration, and angiogenesis are key factors in the wound healing processes. For that reason, controlled promotion of these processes is required. In this work, we present the portable helium-dielectric barrier discharge (He-DBD)-based reaction-discharge system of controlled gas temperature for biological activities. To make this He-DBD-based reaction-discharge system safe for biological purposes, a multivariate optimization of the operating parameters was performed. To evaluate the effect of the He-DBD operating parameters on the rotational gas temperature T rot (OH), a design of experiment followed by a Response Surface Methodology was applied. Based on the suggested statistical model, the optimal operating conditions under which the T rot (OH) is less than 37 °C (310 K) were estimated. Then, the resulted model was validated in order to confirm its accuracy. After estimation the optical operating conditions of He-DBD operation, the spectroscopic characteristic of the He-DBD-based reaction-discharge system in relevance to the several optical temperatures in addition to electron number density has been carried out. Additionally, the qualitative and quantitative analyses of the reactive oxygen species and reactive nitrogen species were performed in order to investigate of reactions and processes running in the He-DBD-gaseous phase and in the He-DBD-treated liquid. Next, the developed portable He-DBD-based reaction-discharge system, working under the optimal operating conditions, was used to stimulate the wound healing process. It was found that a 30 s He-DBD treatment significantly increased the proliferation, migration, and angiogenesis of keratinocytes (HaCaT) and fibroblasts (MSU-1.1) cell lines, as well as human skin microvascular endothelial cells (HSkMEC.2). Hence, the application of the cold atmospheric pressure plasma generated in this He-DBD-based reaction-discharge system might be an alternative therapy for patient suffering from chronic wounds. Keywords Wound healing • Angiogenesis • Migration • Proliferation • Cold atmospheric pressure plasma Abbreviations BBD Box-Behnken design Anna Dzimitrowicz and Aleksandra Bielawska-Pohl have equally contributed to this work.

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Section: Proliferation Assaymentioning

confidence: 99%

Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature

Dzimitrowicz

Bielawska‐Pohl

Jamróz

et al. 2019

Plasma Chem Plasma Process

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“…For these reason recent studies proposed the dog as a study model in comparative oncology [4]. However, the need to reduce animal testing has shifted the attention of the scientific community to cell cultures; in particular, the canine OSA cell line D-17 is often used during in vitro studies [7]. Nevertheless, this line has not been described in terms of basal gene expression and interaction with infectious stressors.…”

Section: Introductionmentioning

confidence: 99%

Characterization of D-17 Canine Osteosarcoma Cell Line and Evaluation of Its Ability to Response to Infective Stressor Used as Alternative Anticancer Therapy

Modesto

Fernandez²,

Martini

et al. 2020

Animals

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Osteosarcoma (OSA) is a rare cancer both in human and dog although the incidence rate in dogs is 27 times higher than in human. Many studies employed D-17 as cell line for in vitro test to evaluate conventional anticancer therapies; however, little is known about D-17 cell line. The aim of our study was to evaluate the basal level of gene expression of pivotal molecules in the innate immune response and cell cycle regulation and to establish the ability of this cell line to react to Salmonella typhimurium (ST) infective stressor. IL15, IL10, iNOS, TLR5, CD14, PTEN and IL18 were expressed in an inconsistent manner among experiments. The other genes under study were expressed in all samples. ST showed ability to penetrate D-17 causing pro-inflammatory response. Our results outline the expression in D-17 of important genes involved in innate immune response. These results provide important data on D-17 basal gene expression profile useful for in vitro preliminary evaluation of new therapeutic approaches.

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“…In vitro chemosensitivities of various malignancies including canine mammary gland cancer, feline injection siteassociated sarcoma (FISAS), and canine osteosarcoma have been reported in only a handful of veterinary publications. [35][36][37] The concentration of carboplatin required to inhibit 50% of cellular activity (IC 50 ), which is viewed as an indicator for expected efficacy of chemotherapeutic agents, was studied. 38 The findings in these studies were consistent; the ability of carboplatin to inhibit cellular activity was both time and concentration dependent with mean IC 50 values ranging from 87.5 μM at 24 hours to 30.5 μM at 72 hours for the mammary gland tumors and 68.3 μM at 24 hours to 34.1 μM at 72 hours for the FISAS and considerably lower values for the osteosarcoma cells.…”

Section: Discussionmentioning

confidence: 99%

“…However, when the clinical application of the results of this study are considered, higher dosing may not be required. In vitro chemosensitivities of various malignancies including canine mammary gland cancer, feline injection site‐associated sarcoma (FISAS), and canine osteosarcoma have been reported in only a handful of veterinary publications 35‐37 . The concentration of carboplatin required to inhibit 50% of cellular activity (IC 50 ), which is viewed as an indicator for expected efficacy of chemotherapeutic agents, was studied 38 .…”

Section: Discussionmentioning

confidence: 99%

Repeatability of in vitro carboplatin elution from carboplatin‐impregnated calcium sulfate hemihydrate beads made in a clinic setting

Worth¹,

Risselada²,

Cooper³

et al. 2020

Veterinary Surgery

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Objective: To evaluate the intra-lot and inter-lot consistency and total carboplatin elution over 25 days from carboplatin-impregnated calcium sulfate hemihydrate (C-I CSH) beads manufactured in a clinic setting. Study design: In vitro elution study. Methods: Two volumes of carboplatin were mixed with CSH to yield 4 mg and 8 mg C-I CSH doses. Two lots of beads were made for each concentration and split into five doses (n = 10 per concentration). Beads hardened in molds and were placed in a covered six-well plate, submerged in phosphate-buffered saline, and incubated with samples collected at 12 time points (0, 6, 12, and 24 hours and 2, 3, 5, 7, 10, 14, 18, and 25 days). The amount of carboplatin in each sample was evaluated by high-performance liquid chromatography/mass spectrometry. Correction for carboplatin degradation and dilution was applied, and eluted carboplatin was calculated. Intra-lot and inter-lot coefficient of variation (CV) was calculated for each concentration. Results: The intra-lot CV ranged between 7.9% and 23.1%, and the inter-lot CV ranged from 3.5% to 10.3%, with improvement noted in each successive lot of beads. Mean peak eluted carboplatin was 2.45 ± 0.43 mg (61%) and 3.68 ± 0.41 mg (45.9%) for the 4-mg and 8-mg C-I CSH beads, respectively, with both occurring at the 12-hour timepoint. Conclusion: Progressive improvement in variability with successive lots of beads indicated a learning curve with bead manufacturing with a low variation both within and between lots of C-I CSH beads. Clinical significance: On-site mixing of carboplatin with commercial CSH bead powder leads to a low variation of carboplatin per bead dose.

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In vitro effects of the activity of novel platinum (II) complex in canine and human cell lines

Cited by 8 publications

References 52 publications

Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature

Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature

Characterization of D-17 Canine Osteosarcoma Cell Line and Evaluation of Its Ability to Response to Infective Stressor Used as Alternative Anticancer Therapy

Repeatability of in vitro carboplatin elution from carboplatin‐impregnated calcium sulfate hemihydrate beads made in a clinic setting

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