In Vitro Effects ofIvermectin and Sulphadiazine on Toxoplasma gondii

Bilgin, Melek; Yıldırım, Tuba Demirci; Hökelek, Murat

doi:10.5152/balkanmedj.2012.098

Cited by 9 publications

(2 citation statements)

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“…Since the approval of the use of ivermectin in humans, the drug has been tested against a variety of human parasitic diseases, as reviewed previously (31,32), but it was suggested that it was inactive against bacteria, fungi, flatworms, and protozoans (33). However, recent publications described in vitro activity of ivermectin against flavivirus (34), bacterial species (35)(36)(37), Toxoplasma gondii (38), and Trypanosoma brucei (39).…”

Section: Discussionmentioning

confidence: 99%

Ivermectin Impairs the Development of Sexual and Asexual Stages of Plasmodium falciparumIn Vitro

Carvalho

Sandri

Melo

et al. 2019

Antimicrob Agents Chemother

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Ivermectin is the drug of choice for many parasitic infections, with more than one billion doses being distributed in onchocerciasis programs. The drug has been put into focus recently by the malaria community because of its potential to kill blood-sucking mosquitoes, thereby reducing malaria transmission. However, the activity of ivermectin against the malaria parasite itself has been only partly investigated. This study aimed to investigate the in vitro activity of ivermectin against asexual and sexual stages of Plasmodium falciparum. Both asexual and late-stage gametocytes were incubated with ivermectin and control drugs in vitro. The growthinhibiting effects were assessed for asexual stages of different Plasmodium falciparum laboratory strains and culture-adapted clinical isolates using the histidine-rich protein 2 enzyme-linked immunosorbent assay technique. The effect against stage IV/V gametocytes was evaluated based on ATP quantification. Ivermectin showed activities at nanomolar concentrations against asexual stages (50% inhibitory concentration of ϳ100 nM) and stage IV/V gametocytes (500 nM) of P. falciparum. Stagespecific assays suggested that ivermectin arrests the parasite cycle at the trophozoite stage. Ivermectin might add a feature to its "wonder drug" properties with activity against asexual stages of the malaria parasite Plasmodium falciparum. The observed activities might be difficult to reach with current regimens but will be more relevant with future high-dose regimens under investigation. Further studies should be performed to confirm these results in vitro and in vivo.

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