In vitro efficacy of allicin on chicken Eimeria tenella sporozoites

Abstract

Chicken coccidiosis is a major parasitic disease caused by Eimeria spp. It is controlled and treated using chemical anticoccidial agents. Development of partial or complete resistance toward these anticoccidials is considered a major problem in poultry industry. Allicin is an organosulfur compound produced as a result of the reaction between alliin and alliinase after hacking of garlic. In this study, tenfold dilution from 180 mg/ml to 1.8 ng/ml of allicin in distilled water was tested against E. tenella in vitro. The percent of inhibition in allicin was from 99.9 to 71.53% using 180 mg/ml and 180 ng/ml, respectively. The percent of inhibition was 56.24% using 1.8 ng/ml. We used allicin as a treatment from plants against chicken coccidiosis; however, in vivo study should be performed to confirm these results.

Herbal Remedies for Coccidiosis Control: A Review of Plants, Compounds, and Anticoccidial Actions

“…Garlic and its sulfur compounds, allicin, alliin, ajoene, diallyl sulfide, dithiin, and allylcysteine, are reported to have broad antimicrobial activities which can eliminate negative factors of microbial infections. An in vitro study has shown that allicin inhibits sporulation of E. tenella effectively [ 26 – 28 ]. The anticoccidial mechanism of garlic and its sulfur compounds remains elusive.…”

Suzuki-Miyaura Coupling between 3-Iodolawsone and Arylboronic Acids. Synthesis of Lapachol Analogues with Antineoplastic and Antileishmanial Activities

“…The crude product was purified by flash column chromatography on silica gel using hexanes/EtOAc (90:10) as eluent. This compound was obtained as yellow needles in 74% yield, mp 160-161 °C; IR (KBr) n / cm - General procedure for the preparation of 1,4-dioxo-3-aryl-1,4-dihydronaphthalen-2-yl diethylcarbamates (7a-e) and 3-(3-methylbut-2-en-1-yl)-1,4-dioxo-1,4-dihydronaphthalen-2-yl diethylcarbamate (8) A stirred solution of 1 (0.1 mmol) or 4 (0.1 mmol), pyridine (5 equiv), N,N-diethylcarbamoyl chloride (20 equiv), in CHCl 3 (10 mL) was heated at 60 °C for 18 hours. Dichloromethane (30 mL) was added to the reaction mixture and the organic layer was first washed with a solution of hydrochloric acid 1N (30 mL) and then with brine (3 × 30 mL), dried over anhydrous Na 2 SO 4 and concentrated in vacuo.…”

“…Eiremia tenella is a protozoan disease that causes poultry coccidiosis impacting directly in chicken industry and global food supply due to the high mortability. 8 Lapachol (1) has been used as starting point to obtain new bioactive quinones showing interesting pharmacological profiles. 9,10 Herein, we would like to report the synthesis of 2-hydroxy-3-arylnaphthalene-1,4-diones, analogues of WS-5995-C that can also be considered analogues of 1 in which the prenyl side chain is substituted by an aromatic ring ( Figure 1).…”

Effect of Calotropis procera (madar) and amprolium supplementation on parasitological parameters of broilers during mixed Eimeria species infection

“…Garlic and its sulfur compounds, i.e., allicin, alliin, ajoene, diallyl sulfide, dithiin, and allylcysteine, are reported to have broad antimicrobial activities. An in vitro study has shown that allicin inhibits sporulation of E. tenella effectively [ 30 ]. Green tea extracts significantly inhibited the sporulation process of coccidian oocysts.…”