In Vitro Efficacy of Trichoderma Isolates Against Some Fungi Causing Fungal Rot Disease of Tomato.

Koka, JahangirAbdullah; Wani, AbdulHamid; Bhat, M. V.; Parveen, Shazia

doi:10.21474/ijar01/3725

Cited by 8 publications

(7 citation statements)

References 8 publications

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“…The plates were incubated at 25 ± 2 °C until full growth of the control. After the period of incubation, the percentage of inhibition of the test pathogen was calculated as described by [ 27 ]. where; R1: mycelial growth in control, R2: mycelial growth in dual culture.…”

Section: Methodsmentioning

confidence: 99%

In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

2021

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Background Faba bean (Vicia faba L.) cultivation is highly challenged by faba bean black root rot disease (Fusarium solani) in high lands of Ethiopia. To ensure sustainable production of faba beans, searching for eco-friendly disease management options is necessary to curb the progress of the disease timely. The indigenous biocontrol agents that suit local environments may effectively strive with in-situ microorganisms and suppress local pathogen strains. This study aimed to screen antagonistic indigenous compatible Trichoderma and Pseudomonas strains against Fusarium solani. In the pathogenicity test, soil-filled pots were arranged in complete random block design and sown with health faba bean seeds. The effect of some fungicides was evaluated against Fusarium by food poisoning methods to compare with the biocontrol agents. The antagonistic efficacy of biocontrol agents and their compatibility was investigated on Potato dextrose agar medium. Results Fusarium solani AAUF51 strain caused an intense root rotting in faba bean plant. The effect of Mancozeb 80% WP at 300 ppm was comparable with Trichoderma and Pseudomonas strains against Fusarium. The mycelial growth of test the pathogen was significantly (P ≤ 0.05) reduced to 86.67 and 85.19% by Trichoderma harzianum AAUW1 and Trichoderma viridae AAUC22 strains in dual culture, respectively. The volatile metabolites of Pseudomonas aeruginosa AAUS31 (77.78%) found the most efficient in reducing mycelial growth of Fusarium followed by Pseudomonas fluorescens AAUPF62 (71.11%) strains. The cell-free culture filtrates of Pseudomonas fluorescens AAUPF62 and Pseudomonas aeruginosa AAUS31 were more efficient than the Trichoderma strain in reducing the growth of Fusarium isolates. There was no zone of inhibition recorded between Trichoderma harzianum AAUW1, Trichoderma viridae AAUC22, Pseudomonas aeruginosa AAUS31, and Pseudomonas fluorescens AAUPF62 strains, hence they were mutually compatible. Conclusions The compatible Trichoderma and Pseudomonas strains showed antagonistic potentiality that could be explored for faba bean protection against black root rot disease and might have a future dual application as biocontrol agents.

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Section: Methodsmentioning

confidence: 99%

In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

2021

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“…Many biocontrol agents are reported to fight R. solani pathogens such as Bacillus subtilis and Trichoderma species, which stop the pathogen's growth or progress with different strategies like production of siderophores, cell lysis enzymes, or by direct intact (hyperparasitism; Wu et al, 2019;El-Benawy et al, 2020;Heflish et al, 2021). Also, various plant extracts are reported for their antifungal activity against R. solani, including a diverse array of bioactive chemicals that are well-known for their antimicrobial and antifungal activity without causing phytotoxic effects (Al-Askar and Rashad, 2010;Santas et al, 2010;Abd-El-Khair and El-Gamal Nadia, 2011;Hamza et al, 2016;Koka et al, 2017;Parveen et al, 2017). The main reasons for using plant extracts or essential oils as antifungal agents are that they come from nature, and pathogens are less likely to become resistant to them.…”

Section: Introductionmentioning

confidence: 99%

Plantago lagopus extract as a green fungicide induces systemic resistance against Rhizoctonia root rot disease in tomato plants

et al. 2022

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Extensive use of chemical control agents and fungicides typically leads to numerous risks to human health and the environment. Using plant extracts as natural substances represents a dual key for the environment and sustainable food production, as it reduces the input of synthetic pesticides into the environment and/or controls plant pathogens. For the first time, a Plantago lagopus ethanolic extract has been characterized and evaluated for its protective and curative effects against Rhizoctonia solani in tomato plants. The results showed that P. lagopus extract (10 μg/ml) completely inhibited R. solani mycelial growth in vitro. At 20 days of post fungal inoculation, the results demonstrated that using P. lagopus extract (100 μg/ml) in vivo enhanced tomato plant growth by significantly increasing shoot and root parameters in protective and curative treatments. Furthermore, the protective and curative treatments significantly reduced the disease index by 18.66 and 38.66%, respectively. Induction of systemic resistance with upregulation of PR-1 and PR-2 and a significant increase in the transcriptional levels of PR-3 and CHS in all P. lagopus extract-treated tomato plants were reported compared to untreated plants. HPLC analysis showed that the most common polyphenolic components detected in P. lagopus extract were rutin (74206.3 mg/kg), naringenin (2388.74 mg/kg), quercetin (1249.13 mg/kg), and p-hydroxybenzoic acid (1035.87 mg/kg). In addition, the ellagic acid (798.47 mg/kg), vanillic acid (752.55 mg/kg), catechol (648.89 mg/kg), cinnamic acid (332.51 mg/kg), ferulic acid (296.32 mg/kg), benzoic acid (295.95 mg/kg), and chlorogenic acid (116.63 mg/kg) were also reported. Our study is the first to show that P. lagopus extract can help plants fight off R. solani fungal infection. Furthermore, the findings imply that using the P. lagopus extract as a natural biocontrol agent could be a sustainable strategy to manage plant fungal diseases.

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“…The mycelial growth of various pathogenic fungi isolated from infected samples of brinjal was found to be inhibited by Trichoderma spp. (Koka et al, 2017). Macrophomina phaseolina isolated from black gram underwent maximum mycelial growth inhibition of 81.55% by an isolate of T. viride (Suthin Raj et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antagonistic Activity of Trichoderma Viride Isolates Against Sclerotinia Sclerotiorum and Their Role in Growth Promotion of Common Bean

Jan¹,

Malik²,

Wani³

et al. 2022

JBiopest

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A study was done to investigate the antagonistic effects of three Trichoderma viride isolates (TV1, TV2 and TV3) on Sclerotinia sclerotiorum. The results revealed that the isolate TV1 proved to be the best in suppressing the mycelial growth by 77% after 7 days of incubation. However 74.91% and 50% inhibition were recorded on 5th and 3rd days of incubation, respectively. The isolate TV2 caused inhibition of 74.77% on 7th day, and 72.66% and 48.33% after 5th and 3rd days of incubation, respectively. 72.54%, 70.41%, and 51.66% inhibition in mycelial growth of the pathogenic fungus was caused by TV3 isolate after 7th, 5th, and 3rd days of incubation respectively. All the test isolates of T. viride showed a significant effect on the vigour index of common bean. The maximum germination percentage was observed on treatment with TV1 isolate (85%) followed by TV2 (83%) and TV3 (81%) respectively, in comparison to untreated seeds which showed a germination percentage of 73%. Maximum vigour index was shown by those plants whose seeds were treated with TV1 (11560), followed by those treated with isolate TV2 (10707) and TV3 (9234), whereas the control plants showed significantly lower vigour index of 7446 in comparison to treated plants.

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In Vitro Efficacy of Trichoderma Isolates Against Some Fungi Causing Fungal Rot Disease of Tomato.

Cited by 8 publications

References 8 publications

In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

Plantago lagopus extract as a green fungicide induces systemic resistance against Rhizoctonia root rot disease in tomato plants

In Vitro Antagonistic Activity of Trichoderma Viride Isolates Against Sclerotinia Sclerotiorum and Their Role in Growth Promotion of Common Bean

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