In vitro efficiency and mechanistic role of indocyanine green as photodynamic therapy agent for human melanoma

Mamoon, A.; Gamal‐Eldeen, Amira M.; Ruppel, M.; Smith, Raymond Τ.; Tsang, T.; Miller, Lisa M.

doi:10.1016/j.pdpdt.2009.05.002

Cited by 50 publications

(35 citation statements)

References 51 publications

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“…A few studies have demonstrated apoptosis post-PDT, but the time required for apoptosis or photosensitization were longer than in the present study [24,25]. The HT treatment duration required to induce apoptosis also ranged from 30 to 90 min [37,43].…”

Section: Discussionmentioning

confidence: 56%

Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG) induces Apoptosis and Cell Cycle Arrest in B16F10 Murine Melanoma Cells

Radzi

Osaki

Tsuka

et al. 2012

The Journal of Veterinary Medical Science

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AbSTRAcT. We examined the effects of photodynamic hyperthemal therapy (PHT), which is a combination of photodynamic therapy (PDT) and hyperthermia (HT), on the apoptosis and cell cycle progression of murine melanoma b16F10 cells. The percentage of apoptotic cell was determined by flow cytometry using fluorescein isothiocyanate (FITC)-conjugated Annexin V and propidium iodide (PI) double staining. The cell cycle analysis was performed by PI staining with flow cytometry. The expression of cyclins and heat shock protein 70 (Hsp70) were examined by a Western blotting analysis. PHT induces death in b16F10 cells, and PHT-mediated apoptosis occurred acutely and persistently in vitro. Our study demonstrated that PHT using indocyanine green (IcG) and near infrared (NIR) light source induces apoptosis and G0/G1 cell cycle arrest in the b16F10 cells.

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Section: Discussionmentioning

confidence: 56%

Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG) induces Apoptosis and Cell Cycle Arrest in B16F10 Murine Melanoma Cells

Radzi

Osaki

Tsuka

et al. 2012

The Journal of Veterinary Medical Science

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“…SR-FTIR analysis was performed on human skin melanoma cells (Sk-Mel-28) treated with ICG followed by PDT. [112] Cluster analysis of the resultant IR spectra revealed a decrease in the nucleic acid phosphate absorption modes at 1240 and 1180 cm 1 in the ICG/PDT-treated cells which was consistent with DNA fragmentation of the apoptotic cells. [112] Additionally, there were protein conformational changes associated with protein denaturation and increases in the lipid bands suggesting redistribution of the phospholipids within the cell membrane (i.e., externalisation of phosphatidyl serine).…”

Section: Anti-cancer Agentsmentioning

confidence: 62%

“…Finally, the use of SR-FTIR microspectroscopy for studying the biomolecular effects of drugs in vivo and in cell culture has become prominent in the last 5 years with studies of anticancer drugs, [92,[108][109]125] osteoporosis drugs, [102] and photodynamic therapeutics [111][112] showing distinguishable biomolecular changes. Issues associated with Mie scatter distortion of the spectra are being addressed, [92,[122][123][124] and the technique is being critically assessed with a goal to using it for preclinical and clinical evaluation of drug candidates.…”

Section: Discussionmentioning

confidence: 99%

“…[92,106] As such, recent attention has been focussed on the use of SR-FTIR microspectroscopy for the study of biomolecular changes that occur following the administration of drugs or potential therapeutic agents in diseased cells. [92,[108][109][110][111][112] Importantly, macromolecules associated with biological samples produce signature IR bands that can be readily discerned from the IR spectra. [94,99,[113][114] Typical spectral features include: amide I ( 1650 cm 1 ) and amide II ( 1549 cm 1 ) bands representative of proteins; symmetric (1080 cm 1 ) and anti-symmetric PO 2  (1232 cm 1 ) bands associated with DNA; C-H stretches of -CH 3 ( as 2955 cm 1 ) and -CH 2 ( as 2850 cm 1 ), the ester band (1740 cm 1 ) and the C-O-P (1080 cm 1 ) band associated with lipids/phospholipids; and C-O stretches (1050 cm 1 ) of carbohydrates.…”

Section: Synchrotron Radiation -Fourier Transform Infrared Microspectmentioning

confidence: 99%

“…[111] The PDT potential of 4,5-benzoindotricarbocyanine (indocyanine green, ICG) as a treatment for human melanoma was also assessed by SR-FTIR microspectroscopy. [112] Previously, the poor success of PDT with melanomas was believed to stem from their high content of melanin which acts as a screen for white light. Consequently, ICG was proposed since it absorbs light in the range 700-800 nm, where melanins do not absorb.…”

Section: Anti-cancer Agentsmentioning

confidence: 99%

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Synchrotron Radiation Spectroscopic Techniques as Tools for the Medicinal Chemist: Microprobe X-Ray Fluorescence Imaging, X-Ray Absorption Spectroscopy, and Infrared Microspectroscopy

Dillon

2012

Aust. J. Chem.

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. (2012). Synchrotron radiation spectroscopic techniques as tools for the medicinal chemist: microprobe X-Ray fluorescence imaging, X-Ray absorption spectroscopy, and infrared microspectroscopy. Australian Journal of Chemistry: an international journal for chemical science, 65 (3), 204-217. Synchrotron radiation spectroscopic techniques as tools for the medicinal chemist: microprobe X-Ray fluorescence imaging, X-Ray absorption spectroscopy, and infrared microspectroscopy AbstractThis review updates the recent advances and applications of three prominent synchrotron radiation techniques, microprobe X-ray fluorescence spectroscopy/imaging, X-ray absorption spectroscopy, and infrared microspectroscopy, and highlights how these tools are useful to the medicinal chemist. A brief description of the principles of the techniques is given with emphasis on the advantages of using synchrotron radiation-based instrumentation rather than instruments using typical laboratory radiation sources. This review focuses on several recent applications of these techniques to solve inorganic medicinal chemistry problems, focusing on studies of cellular uptake, distribution, and biotransformation of established and potential therapeutic agents. The importance of using these synchrotron-based techniques to assist the development of, or validate the chemistry behind, drug design is discussed. AbstractThis review updates the recent advances and applications of three prominent synchrotron radiation techniques, microprobe X-ray fluorescent spectroscopy/imaging, X-ray absorption spectroscopy and infrared microspectroscopy, and highlights how these tools are useful to the medicinal chemist. A brief description of the principles of the techniques is given with emphasis on the advantages of using synchrotron radiation-based instrumentation rather than instruments using typical laboratory radiation sources. This review focuses on a number of recent applications of these techniques to solve inorganic medicinal chemistry problems, focusing on studies of cellular uptake, distribution and biotransformation of established and potential therapeutic agents. The importance of using these synchrotron-based techniques to assist the development, or validate the chemistry behind, drug design is discussed.3

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Use of Carbon Dioxide Laser to Treat Lentigo Maligna and Malignant Melanoma In Situ, Lentigo Maligna Type

McLeod¹,

França²,

Ferris³

et al. 2012

Archives of Facial Plastic Surgery

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In vitro efficiency and mechanistic role of indocyanine green as photodynamic therapy agent for human melanoma

Cited by 50 publications

References 51 publications

Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG) induces Apoptosis and Cell Cycle Arrest in B16F10 Murine Melanoma Cells

Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG) induces Apoptosis and Cell Cycle Arrest in B16F10 Murine Melanoma Cells

Synchrotron Radiation Spectroscopic Techniques as Tools for the Medicinal Chemist: Microprobe X-Ray Fluorescence Imaging, X-Ray Absorption Spectroscopy, and Infrared Microspectroscopy

Use of Carbon Dioxide Laser to Treat Lentigo Maligna and Malignant Melanoma In Situ, Lentigo Maligna Type

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