In vitro endothelial cell response to ionic dissolution products from boron-doped bioactive glass in the SiO₂–CaO–P₂O₅–Na₂O system

Abstract

As it has been established that boron (B) may perform functions in angiogenesis and osteogenesis, the controlled and localized release of B ions from bioactive glasses (BGs) is expected to provide a promising therapeutic alternative for regenerative medicine of vascularized tissues, such as bone. The aim of this study was to assess the in vitro angiogenic effects of the ionic dissolution products (IDPs) from BGs in the SiO 2 -CaO-Na 2 O-P 2 O 5 (45S5) system and of those from 45S5 BG doped with 2 wt%

Angiogenic effects of ionic dissolution products released from a boron-doped 45S5 bioactive glass

“…in vitro HUVECs tubule formation and secretion of interleukin 6 (IL6) and bFGF. 18 The effects observed in the present study are probably due to the fact that borate stimulates the secretion of cytokines and/or growth factors with pro-angiogenic activity and the proliferation of endothelial cells of the CAM vasculature, as evidenced by the higher expression of integrin a v b 3 . The process of angiogenesis involves coordinated endothelial cell proliferation, migration and tube formation.…”

“…16,18 The base composition selected for the preparation of these BGs complies with the following characteristics: (a) it is bioactive and (b) it belongs to the SiO 2 -CaO-Na 2 O-P 2 O 5 system. The BGs were prepared from the following raw materials: SiO 2 , CaCO 3 , Na 2 CO 3 , CaHPO 4 $2H 2 O, and B 4 Na 2 O 7 $10H 2 O.…”

“…The raw materials were mixed in the relationship necessary to obtain the desired composition in a base 45S5 BG containing 45% (in weight%) SiO 2 , 24.5% Na 2 O, 24.5% CaO, and 6% P 2 O 5 , and to which 2% of B 2 O 3 (45S5.2B) was added to partially replace SiO 2 . 16,18 Mixing was carried out in a platinum crucible at 1350 C using an electric oven (Carbolite RHF 17/6S, Carbolite Ltd, England) holding the temperature for 3 h to allow the fusion of the components and the homogenization of the glass. The molten glass was quenched on graphite plates to allow fast cooling, and thus preventing its crystallization.…”

“…[16][17][18] Noteworthy, Brown et al 19 reported that with increasing B 2 O 3 content, greater than $1/3 of the SiO 2 in the 45S5 BG system, the glasses produced a greater inhibition of cell proliferation, particularly in static culture conditions.…”

Bioactive Glass-Biopolymer Composites for Applications in Tissue Engineering

“…Furthermore, some trace elements when incorporated into bioactive glass compositions have been shown to provide additional properties that can be beneficial for tissue regeneration. For instance, zinc is responsible for vitamin A and E metabolism; magnesium can activate the phosphate-transferring enzyme; copper can help the formation of myelin sheaths for the neurological system, and vitamin B can enhance angiogenesis [18]. Therefore, numerous ion-doped bioactive glasses are being developed, which exhibit high biological activity and tissue regeneration capability [10,33].…”