In vitro equine oocyte maturation in pure follicular fluid plus interleukin-1 and fertilization following ICSI

Caillaud, Maud; Dell’Aquila, Maria Elena; Santis, Teresa De; Nicassio, M.; Lacalandra, Giovanni Michele; Goudet, Ghylène; Gérard, Nadine

doi:10.1016/j.anireprosci.2007.06.005

Cited by 15 publications

(12 citation statements)

References 27 publications

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“…Furthermore, embryo cleavage and implantation rates may be improved by higher follicular fluid IL‐1β concentrations (Karagouni et al, ; Mendoza et al, ). Yet, other studies in humans and horses have found no correlation between follicular fluid IL‐1β and oocyte maturation, fertilisation, or embryo cleavage rates, bringing the benefits of high levels of IL‐1β into question (Mendoza et al, ; Caillaud et al, ; Bedaiwy et al, ).…”

Section: Clinical Relevance Of Cytokine Network In Folliculogenesismentioning

confidence: 99%

Cytokines in ovarian folliculogenesis, oocyte maturation and luteinisation

Field

Dasgupta

Cummings

et al. 2013

Molecular Reproduction Devel

166

110

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SUMMARYCytokines are key regulators of ovarian physiology, particularly in relation to folliculogenesis and ovulation, where they contribute to creating an environment supporting follicle selection and growth. Their manifold functions include regulating cellular proliferation/differentiation, follicular survival/atresia, and oocyte maturation. Several cytokines, such as TGF-b-superfamily members, are involved at all stages of folliculogenesis while the production of others is stage-dependent. This review draws upon evidence from both human and animal models to highlight the species-specific roles at each milestone of follicular development. Given these pivotal roles and their ease of detection in follicular fluid, cytokines have been considered as attractive biomarkers of oocyte maturational status and of successful assisted reproductive outcome. Despite this, our understanding of cytokines and their interactions remains incomplete, and is still frequently limited to overly simplistic descriptions of their interrelationships. Given our increased appreciation of cytokine activity in complex and highly regulated networks, we put forward the case for using Bayesian modelling approaches to describe their hierarchical relationships in order to predict causal physiological interactions in vivo.Mol. Reprod. Dev. 81: 284À314,

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Section: Clinical Relevance Of Cytokine Network In Folliculogenesismentioning

confidence: 99%

Cytokines in ovarian folliculogenesis, oocyte maturation and luteinisation

Field

Dasgupta

Cummings

et al. 2013

Molecular Reproduction Devel

166

110

View full text Add to dashboard Cite

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“…Interleukin‐1 appeared to induce meiosis and ovulation when injected into the preovulatory follicle in vivo, but depressed pregnancy rates (Caillaud et al, 2005). Similarly, interleukin‐1 depressed fertilization rates and embryo development in vitro (Caillaud et al, 2008). Addition of cysteamine to maturation medium did not affect glutathione content of oocytes or rate of oocyte maturation, and maturation rate was not associated with glutathione content (Luciano et al, 2006).…”

Section: Developmental Competencementioning

confidence: 99%

The equine oocyte: Factors affecting meiotic and developmental competence

Hinrichs

2010

Molecular Reproduction Devel

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SUMMARYThere is currently much interest in assisted reproduction techniques in the horse, however, many aspects of oocyte maturation, fertilization, and embryo development in the horse differ from those in other species. Because of the close attachment of the equine oocyte to the follicle wall, scraping of the follicle is the most effective method for oocyte recovery. A notable feature of equine oocytes is that those with expanded cumuli (Ex oocytes), which originate from atretic follicles, have higher meiotic competence (ability to mature to metaphase II in vitro) than do oocytes with compact cumuli (Cp oocytes). Cp oocytes originate in viable follicles but are largely juvenile. Recovery and culture of equine oocytes immediately after slaughter yields a higher maturation rate than that obtained from oocytes after ovary storage; this is related to damage to chromatin in Cp oocytes during storage. In contrast, developmental competence (rate of blastocyst development in vitro) is higher in oocytes recovered from the ovary after a delay. The optimum duration of maturation varies based on cumulus morphology and time of recovery from the ovary, but there is no difference in developmental competence between Ex and Cp oocytes. Because standard in vitro fertilization is not repeatable in the horse, oocyte transfer (surgical transfer of oocytes to the oviducts of inseminated mares) has been developed to allow fertilization of isolated oocytes. Fertilization in vitro may be achieved using intracytoplasmic sperm injection; culture of injected oocytes in a medium with high glucose can yield over 30% blastocyst development.Mol. Reprod. Dev. 77: 651-661,

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“…Moreover, FF provides a complex and peculiar environment for follicular growth, representing the metabolic and secretory activity of granulosa and thecal cells. Circumstantial evidence suggests that the evaluation of FF components, like steroid hormones, may provide additional information on their metabolic and dynamic changes, including into systemic concentrations [26,27].…”

Section: Discussionmentioning

confidence: 99%

“…The biochemical FF composition of large follicles represents a significant marker of the metabolic and secretory pattern of follicular cells, with an applicative guide in formulating cell culture topics [35]. Since the maturation of the oocytes in the mare involves specific mechanisms of the species, some of which may be related to the steroids contained in the equine FF, it is of crucial importance to oocyte preparation for fertilisation and development [26,41]. The development of in vitro culture conditions able to mimic the maturation of the oocyte may help to improve embryo production.…”

Section: Discussionmentioning

confidence: 99%

Can the Presence of Ovarian Corpus Luteum Modify the Hormonal Composition of Follicular Fluid in Mares?

Satué

Fazio

Medica

2020

Animals

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The hypothesis of this study was to investigate if the presence of corpus luteum (CL) in one ovary could modify the hormonal content of follicular fluid (FF) in the follicles. Sixty ovaries were taken after the slaughter of 30 clinically healthy mares. In relation to the sizes, the follicles were classified into three different categories, as small (20–30 mm), medium (31–40 mm) and large (≥41 mm). Blood samples were collected from the jugular vein of mares before their slaughter, and then the FF samplings were extracted from each single follicle. The ovaries that were collected were classified into two groups, according to the presence (CL-bearing) or absence (non-CL-bearing) of CL. The serum and FF samples were analysed for progesterone (P4), oestradiol-17β (E2), testosterone (T), androstenedione (A4) and dehydroepiandrosterone (DHEA). Intrafollicular P4 concentrations in large follicles of CL-bearing groups were lower than for non-CL-bearing ones. Intrafollicular E2 concentrations increased with the increase of the follicle diameter in both groups, CL-bearing and non-CL-bearing. However, in the FF with a large and medium follicle size, E2 concentrations were significantly higher in non-CL-bearing groups than in CL-bearing groups. T and A4 significantly increased in the large and medium follicle sizes when compared to the small follicle sizes in both groups, but higher concentrations in the non-CL-bearing group were obtained. Intrafollicular DHEA significantly decreased with the increase of the follicular diameter in both groups. Steroid hormones in FF dynamically changed, according to the presence or not of CL in the ovary. This study brings new knowledge on the role of the CL in the follicular hormonal composition in mares.

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In vitro equine oocyte maturation in pure follicular fluid plus interleukin-1 and fertilization following ICSI

Cited by 15 publications

References 27 publications

Cytokines in ovarian folliculogenesis, oocyte maturation and luteinisation

Cytokines in ovarian folliculogenesis, oocyte maturation and luteinisation

The equine oocyte: Factors affecting meiotic and developmental competence

Can the Presence of Ovarian Corpus Luteum Modify the Hormonal Composition of Follicular Fluid in Mares?

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