In Vitro Evaluation of Antimicrobial Activity of Minocycline Formulations for Topical Application in Periodontal Therapy

Schmid, Jan-Luca; Kirchberg, Martin; Sarembe, Sandra; Kiesow, Andreas; Sculean, Anton; Mäder, Karsten; Buchholz, Mirko; Eick, Sigrun

doi:10.3390/pharmaceutics12040352

Cited by 17 publications

(14 citation statements)

References 47 publications

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“…The oral cavity is colonized by microflora [22] consisting of bacteria, viruses, and fungi. Approximately 500 species of bacteria exist in the oral cavity [3]. In particular, a fungus known as C. albicans inhabits the oral cavity; if a large number of this type of fungus exists, an infection called candidiasis becomes highly probable [2].…”

Section: Discussionmentioning

confidence: 99%

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Analysis of the Antimicrobial, Cytotoxic, and Antioxidant Activities of Cnidium officinale Extracts

Lee

Kang

2020

Plants

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This study analyzed the antimicrobial, cytotoxic, and antioxidant properties of Cnidiumofficinale (CO) extracts to confirm their antimicrobial activity toward oral microorganisms. The control group contained 0 μg/mL of CO, and the experimental groups contained 50, 100, 150, and 200 μg/mL of CO. To confirm the antibacterial activity of CO extracts against microorganisms in the oral cavity, an inhibition zone test, a colony-forming unit (CFU) analysis, an optical density (OD) evaluation, and a SEM (scanning electron microscopy) analysis were performed. A cytotoxicity test was also conducted to determine cell viability, and the contents of flavonoids and polyphenols were measured to analyze the extract components. In the control group, the growth inhibition zone increased, while the CFU and OD values decreased (p < 0.05). The SEM analysis confirmed that the number of microorganisms for both the microbes decreased. The cell viability was more than 80% in both the control and experimental groups, excluding the 200 μg/mL sample. The flavonoid and polyphenol contents in the experimental groups showed higher values than those of the control group. Therefore, the CO extract showed considerable antimicrobial activity toward both Streptococcus mutans and Candida albicans, suggesting that it may be used as a natural antimicrobial agent for dental applications.

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Section: Discussionmentioning

confidence: 99%

“…The oral cavity contains various kinds of bacteria and fungi [1,2]. Chemicals, such as antibiotics, are used to suppress oral bacteria [3,4]. However, antibiotics have harmful effects on the human body and can result in antibiotic resistance [5].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Antimicrobial, Cytotoxic, and Antioxidant Activities of Cnidium officinale Extracts

Lee

Kang

2020

Plants

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“…The aetiology of periodontitis is closely linked to the manifestation of proinflammatory bacterial dysbiosis through the overgrowth of specific, mostly gram-negative, germs in bacterial biofilms. Therefore, the reduction of bacterial biofilm must be the aim of any established periodontal therapy [ 29 ]. As mechanical cleaning alone cannot fully eliminate all bacteria involved in periodontal disease, antibiotics are applied to the treatment of periodontal disease.…”

Section: Discussionmentioning

confidence: 99%

Production and characteristics of a novel chicken egg yolk antibody (IgY) against periodontitis-associated pathogens

et al. 2020

Journal of Oral Microbiology

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Periodontitis is a bacterial biofilm-induced oral disease, mostly caused by Aggregatibacter actinomycetemcomitans ( A. actinomycetemcomitans ) and Porphyromonas gingivalis ( P. gingivalis ). Oral administration of chicken egg yolk antibody (IgY) is a promising nutritional strategy to control pathogen infections. The objective of this study was to produce an A. actinomycetemcomitans - and P. gingivalis -specific IgY and evaluate its effects on bacterial agglutination and biofilm formation. Thirty laying hens were immunized with a complex of lysate containing typical molecular weights of membrane proteins of A. actinomycetemcomitans and P. gingivalis . IgY was isolated by polyethylene glycol 6000 and ammonium sulfate and purified by dialysis. The results of enzyme-linked immunosorbent assay showed that the obtained IgY were specific to both A. actinomycetemcomitans and P. gingivalis . In addition, immunoelectron microscopy scanning and crystal violet staining showed that the IgY could bind to cell wall of the pathogens and efficiently accelerate agglutination and inhibit biofilm formation. Furthermore, the activity of the IgY remained stable at different temperature, pH, and storage period. This is the first report that a novel two-in-one IgY was produced to modulate the agglutination and biofilm formation of A. actinomycetemcomitans and P. gingivalis , suggesting the potential of IgY to control periodontitis caused by oral pathogens.

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“… 7 Vaccines are being tested as a potential therapy, but there are no specific vaccines or other treatments for COVID-19 until now. 8 …”

Section: Introductionmentioning

confidence: 99%

Dental Practice during COVID-19 in Nepal: A Descriptive Cross-sectional Study

Humagain

Humagain²,

Rokaya

2020

J Nepal Med Assoc

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Introduction: Currently, coronavirus disease (COVID-19) has become pandemic and spread globally. In Nepal, the number of COVID-19 is increasing day-by-day. This research was done to find out the impact of COVID-19 on dentists, patients, and dental practice in Nepal. Methods: This study is a cross-sectional study conducted using an online survey from May 10 to17, 2020. A questionnaire was designed and uploaded in Freeonlinesurveys.com. Following ethical approval, the questionnaire was distributed among 500 dentists, and 406 dentists participated in the study. The survey link was dispersed to the Nepali dentists through social media and e-mail, and the results of the responses were received online. The questionnaire consisted of a total of 34 closed-ended questions containing three parts; demographic details, knowledge of dentists on COVID-19, and the impact of COVID-19 on dentists, patients, and dental treatments. Results: It showed that majority of the participants were females 243 (60%) of the age group 25-29 years with the clinic as the workplace. Patients receive dental treatments only from 40 (10%) of the dentist. A high number of dentists: 284 (70%) were severely affected by the financial burden and were not receiving a salary during this lockdown. About 349(86%) of the dentist think they should do regular dental treatments, but only 101 (25%) think the dentist should do only dental emergency treatments for COVID-19 infected cases. Conclusions: Dentists, patients, and dental practice are severely affected by the COVID-19.The majority of the dentists have faced financial burdens. The dental treatments should be done with high standards of care and infection control following proper recommendations.

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In Vitro Evaluation of Antimicrobial Activity of Minocycline Formulations for Topical Application in Periodontal Therapy

Cited by 17 publications

References 47 publications

Analysis of the Antimicrobial, Cytotoxic, and Antioxidant Activities of Cnidium officinale Extracts

Analysis of the Antimicrobial, Cytotoxic, and Antioxidant Activities of Cnidium officinale Extracts

Production and characteristics of a novel chicken egg yolk antibody (IgY) against periodontitis-associated pathogens

Dental Practice during COVID-19 in Nepal: A Descriptive Cross-sectional Study

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