In vitro evolution of an atrazine-degrading population under cyanuric acid selection pressure: Evidence for the selective loss of a 47kb region on the plasmid ADP1 containing the atzA, B and C genes

Changey, Frédérique; Devers‐Lamrani, Marion; Rouard, Nadine; Martin-Laurent, Fabrice

doi:10.1016/j.gene.2011.09.005

Cited by 20 publications

(20 citation statements)

References 37 publications

(50 reference statements)

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“…The different patterns are represented as rectangles. The Ar-A6, promoter (P), and Shine-Dalgarno sequence (SD) are indicated (Boubakri et al 2006) (Copyright: Elsevier) depletion of the selection pressure is in favor of the hypothesis of the loss or at least of the dilution of the genetic potential within the pesticide-degrading community (Changey et al 2011), suggesting that pesticide-degrading ability remains a versatile function. Finally, among the techniques associated to the bioaugmentation, there is one relying on the use of killed E. coli recombinant strains that have been genetically modified to overexpress the atzA gene (Strong et al 2000).…”

Section: Resultsmentioning

confidence: 99%

“…ADP, the involvement of IS in the duplication of the gene atzB was shown to improve the rate of mineralization of atrazine giving a gain of fitness to the newly evolved population under atrazine selection pressure (Devers et al 2008). Conversely, under cyanuric acid selection pressure, homologous recombination mediated by ISPps1 led to the selective loss of a 47-kb fragment containing the atzABC genes (Changey et al 2011). In addition, the full sequencing of the pTC1 plasmid of Arthrobacter aurescens TC1 showed the existence of six copies of the gene trzN which are most likely responsible for the high atrazine-degrading efficiency of this strain.…”

Section: Genetic Basis Of Pesticide Biodegradationmentioning

confidence: 97%

“…In several cases, accelerated biodegradation was shown to diminish the efficacy of pesticide treatment: this phenomenon is particularly well described for the insecticide carbofuran (Felsot et al 1982). The stability of pesticide-degrading capabilities observed in situ is in contradiction with the frequently reported instability of degrading capabilities of pure microbial strains maintained under laboratory conditions (Changey et al 2011). Indeed, it has been observed by different researchers that pesticidedegrading potential can easily be lost under laboratory conditions by simply sub-cultivating degrading strains on agar medium without the selection pressure exerted by the pesticide.…”

Section: Genetic Basis Of Pesticide Biodegradationmentioning

confidence: 99%

“…Indeed, it has been observed by different researchers that pesticidedegrading potential can easily be lost under laboratory conditions by simply sub-cultivating degrading strains on agar medium without the selection pressure exerted by the pesticide. Recently, Changey et al (2011) suggested that in absence of the selection pressure, pesticide-degradinggenetic potential represents a genetic burden favoring the selection of strains having selectively lost this burden by different genetic mechanisms. Keeping in mind this instability observed on pure strains kept under laboratory conditions, several hypotheses have been drawn to tentatively explain the stability of pesticide-degrading ability of soil microbial community observed in situ.…”

Section: Genetic Basis Of Pesticide Biodegradationmentioning

confidence: 99%

See 3 more Smart Citations

Applied Microbial Ecology and Bioremediation

Bertrand

Doumenq²,

Guyoneaud

et al. 2014

Environmental Microbiology: Fundamentals and Applications

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The large diversity of metabolic capacities and the high genetic plasticity of microorganisms allow them to degrade virtually all organic compounds of natural or anthropogenic (xenobiotics) origin including those that are sources of environmental pollution. Thus microorganisms are major actors to eliminate or alleviate pollutions in the environment. The natural attenuation processes due to microbial activities (biodegradation and/or biotransformation) as well as the possibilities of using microorganisms in preventive treatments and bioremediation -biostimulation, bioaugmentation, rhizostimulation, bioleaching, and bioimmobilization -are presented. The main methods for microbial treatment of pollution, the chemical structure and the origin of the major pollutants, as well as the mechanisms of degradation by microorganisms -on the basis of physiological, biochemical, and genetic approaches -are described. Examples of treatments are presented for urban wastewater (activated sludge, lagoons, and planted beds), solid wastes (aerobic treatment or composting, anaerobic treatment and methanization, discharges), gaseous effluents, pesticides, polychlorobiphenyls, and finally hydrocarbons and petroleum products in the marine environment.

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Section: Resultsmentioning

confidence: 99%

Section: Genetic Basis Of Pesticide Biodegradationmentioning

confidence: 97%

Section: Genetic Basis Of Pesticide Biodegradationmentioning

confidence: 99%

Section: Genetic Basis Of Pesticide Biodegradationmentioning

confidence: 99%

See 2 more Smart Citations

Applied Microbial Ecology and Bioremediation

Bertrand

Doumenq²,

Guyoneaud

et al. 2014

Environmental Microbiology: Fundamentals and Applications

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show abstract

“…It is worth noting that the lower fitness of adapted populations can lead to counter-selection, i.e. selection pressure against the genetic adaptation, which leads to loss of the adaptation and its associated cost, and restoration of the population fitness (Changey et al, 2011).…”

Section: 3mentioning

confidence: 99%

Recovery in environmental risk assessments at EFSA

Brock¹

2016

EFS2

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EFSA performs environmental risk assessments (ERAs) for single potential stressors such as plant protection products, genetically modified organisms and feed additives and for invasive alien species that are harmful for plant health. In this risk assessment domain, the EFSA Scientific Committee recognises the importance of more integrated ERAs considering both the local and landscape scales, as well as the possible co‐occurrence of multiple potential stressors that fall under the remit of EFSA, which are important when addressing ecological recovery. In this scientific opinion, the Scientific Committee gathered scientific knowledge on the potential for the recovery of non‐target organisms for the further development of ERA. Current EFSA guidance documents and opinions were reviewed on how ecological recovery is addressed in ERA schemes. In addition, this scientific opinion is based on expert knowledge and data retrieved from the literature. Finally, the information presented in this opinion was reviewed by experts from the relevant EFSA Panels, European risk assessment bodies and through an open consultation requesting input from stakeholders. A conceptual framework was developed to address ecological recovery for any assessed products, and invasive alien species that are harmful for plant health. This framework proposes an integrative approach based on well‐defined specific protection goals, scientific knowledge derived by means of experimentation, modelling and monitoring, and the selection of focal taxa, communities, processes and landscapes to develop environmental scenarios to allow the assessment of recovery of organisms and ecological processes at relevant spatial and temporal scales.

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Evolution of atrazine-degrading capabilities in the environment

Udiković-Kolić

Scott

Martin-Laurent³

2012

Appl Microbiol Biotechnol

130

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Since their first introduction in the mid 1950s, man-made s-triazine herbicides such as atrazine have extensively been used in agriculture to control broadleaf weed growth in different crops, and thus contributed to improving crop yield and quality. Atrazine is the most widely used s-triazine herbicide for the control of weeds in crops such as corn and sorghum. Although atrazine was initially found to be slowly and partially biodegradable, predominantly by nonspecific P450 monoxygenases which do not sustain microbial growth, microorganisms gradually evolved as a result of repeated exposure, started using it as a growth substrate and eventually succeeded in mineralizing it. Within three decades, an entirely new hydrolase-dependent pathway for atrazine mineralization emerged and rapidly spread worldwide among genetically different bacteria. This review focuses on the enzymes involved in atrazine mineralization and their evolutionary histories, the genetic composition of microbial populations involved in atrazine degradation and the biotechnologies that have been developed, based on these systems, for the bioremediation of atrazine contamination in the environment.

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In vitro evolution of an atrazine-degrading population under cyanuric acid selection pressure: Evidence for the selective loss of a 47kb region on the plasmid ADP1 containing the atzA, B and C genes

Cited by 20 publications

References 37 publications

Applied Microbial Ecology and Bioremediation

Applied Microbial Ecology and Bioremediation

Recovery in environmental risk assessments at EFSA

Evolution of atrazine-degrading capabilities in the environment

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