In vitro Experiments Revealed the Anthelmintic Potential of Herbal Complex against Haemonchus contortus

Zaman, M. A.

doi:10.29261/pakvetj/2019.128

Cited by 11 publications

(9 citation statements)

References 5 publications

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“…This study's findings are similar to those of Ferreira et al [21], who reported that the leaf extract of Annona muricata L. could completely inhibit the motility of adult H. contortus worms in the first 6-8 h. Ahmed et al [22] stated that Artemisia herba-alba and Punica granatum had a significant effect on the mortality rate of adult H. contortus after 7 h of exposure. Zaman et al [23] reported that herbal complex extracts could cause 100% death in adult H. contortus worms 8 h post-exposure. Widiarso et al [24] Different superscripts indicate significant differences (p<0.05).…”

Section: Discussionmentioning

confidence: 99%

Anthelmintic effect of Indigofera tinctoria L on Haemonchus contortus obtained from sheep in Indonesia

et al. 2021

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Background and Aim: Hemonchosis has resulted in huge economic losses for sheep farmers worldwide. Secondary metabolite compounds from Indigofera tinctoria L. can be used as anthelmintics. This study aimed to evaluate the in vitro and in vivo effects of I. tinctoria L. aqueous extract (IAE) as an anthelmintic against adult Haemonchus contortus isolated from sheep. Materials and Methods: Ten active adult worms were placed in each Petri dish containing 25 mL of IAE, each having a different concentration of IAE (at concentrations 100, 120, 140, 160, 180, 200, and 220 mg/mL). Each experiment was repeated. The positive control used 1% albendazole, and the negative control used 0.62% saline water. The number of immobile worms and the time of mortality were recorded after 2, 4, 6, and 8 h. The dead worms were subsequently tested using scanning electron microscopy (SEM) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In the in vivo study, 15 sheep with an average fecal egg count (FEC) of 1000 eggs per gram were treated with IAE for 9 days: Group A (negative control, saline water 0.62%), Group B (21 mg/mL), Group C (41 mg/mL), Group D (62 mg/mL), and Group E (positive control, albendazole 100 mg/mL). Measurements of the body weight, FEC reduction test, and hematology testing were performed on days 0, 7, and 14. SEM was performed using worms found from the abomasum of slaughtered sheep. Results: The leaves of I. tinctoria L. contained a number of secondary metabolites, including total tannins, saponins, flavonoids, and alkaloids. The most effective concentration that killed the adult H. contortus worms was 220 mg/mL (93.33% mortality) after 8 h of treatment. The electrophoresis results showed that the protein band at a dose of 22% was less than that of the control. The highest FECR value of the treatment group on the 14th day after treatment was at a dose of 62 mg/mL. The highest weight gain as well as the highest increased hemoglobin (Hb), packed cell volume (PCV), and total erythrocyte count (TEC) values on the 14th day after treatment were at a dose of 41 mg/mL. The SEM results showed that IAE treatment caused the worms' anterior parts to become wrinkled with thick creases and cuticle abrasion (in vitro) and the anterior part to shrink along with the presence of aggregates in the worm cuticle (in vivo). Conclusion: The aqueous extract of I. tinctoria contains tannins, saponins, flavonoids, and alkaloids and has an anthelmintic effect with decreased FEC, increased weight gain, Hb, PCV, and TEC, causing damage to the worms' body and reducing the protein profile of adult H. contortus worms.

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Section: Discussionmentioning

confidence: 99%

Anthelmintic effect of Indigofera tinctoria L on Haemonchus contortus obtained from sheep in Indonesia

et al. 2021

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“…These plant products are mostly extracted from flowers and leaves of various aromatic plants growing in temperate and warm regions such as the Mediterranean, the Amazon or tropical countries, where they represent an important part of traditional pharmacopoeia (Bakkali 2008). The medicinal properties of EOs are recognized since ancient times which include antiviral, antibacterial, antifungal, antiparasitic, anti-inflammatory, antiseptic, anticancer and antispasmodic properties among others (Bakkali 2008;Mancianti and Ebani 2020;Zaman et al 2020). In veterinary medicine, EOs are increasingly used for the prevention and treatment of various animal diseases.…”

Section: Properties and Chemical Composition Of Essential Oilsmentioning

confidence: 99%

The Use of Essential Oils Against Sheep Gastrointestinal Nematodes

2022

Int J Vet Sci

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“…In recent years, botanical anthelmintics have emerged as an promising alternative in the control of ruminant GINs and as a tool to combat AR [8,[14][15][16][17]. Among other plant products, essential oils (EOs) are the most commonly mentioned and examined within that context [18].…”

Section: Introductionmentioning

confidence: 99%

“…These natural products have a long history of medical application in humans and are often utilized in many medical practices around the world. The medicinal properties of EOs include anti-inflammatory, antiseptic, anticancer, antispasmodic (and other positive impacts on the gastrointestinal tract), and even anti-anxiety properties, along with antibacterial, antifungal and antiparasitic effects [17,[20][21][22][23]. In veterinary medicine, EOs are increasingly used for the treatment and prevention of various diseases, but are still preferable in monogastric animals such as pigs and poultry [22,[24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep—In Vitro and In Vivo Studies

Štrbac

Bosco

Maurelli

et al. 2022

Veterinary Sciences

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Herbal products such as essential oils may play a promising role in the treatment of infections caused by gastrointestinal nematodes (GINs). The aim of this study was to evaluate the in vitro potential of 11 essential oils (EOs) and one binary combination of isolated EO compounds, as well as the in vivo anthelmintic efficacy of two EO formulations. Four GIN genera were identified in the coproculture examination: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The in vitro egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO, whereas in the in vivo faecal egg count reduction test (FECRT), each EO sample was diluted in sunflower oil and orally administrated at a dose of 100 mg/kg to the different group of animals. In the EHT, the EOs of Origanum vulgare, Foeniculum vulgare, Satureja montana, Satureja hortensis and two types of Thymus vulgaris were the most effective. The dominant compounds of these EOs were carvacrol, thymol, anethol, p-cymene and γ-terpinene, indicating their importance for the anthelmintic activity. In the FECRT, both T. vulgaris EO type 1 and linalool:estragole combination show an anthelmintic potential with a mean effect on FECR of approximately 25%. The results suggest the possible role of tested EOs as anthelmintic agents in sheep farms, although further in vivo tests are needed.

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In vitro Experiments Revealed the Anthelmintic Potential of Herbal Complex against Haemonchus contortus

Cited by 11 publications

References 5 publications

Anthelmintic effect of Indigofera tinctoria L on Haemonchus contortus obtained from sheep in Indonesia

Anthelmintic effect of Indigofera tinctoria L on Haemonchus contortus obtained from sheep in Indonesia

The Use of Essential Oils Against Sheep Gastrointestinal Nematodes

Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep—In Vitro and In Vivo Studies

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