In Vitro Free Radical Scavenging Potential of Trimad (A Polyherbal Formulation)

Abstract

The objective of the present study was to evaluate aqueous extract of Trimad for its in vitro antioxidant activity. A battery of in vitro biochemical tests such as total phenol content, ferric reducing power, ABTS [2, 2 azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)] scavenging activity and Lipid peroxidation inhibitory activity was used. The total phenol content of Trimad was found to be 279.5 mg/g dry mass. In case of DPPH scavenging activity, Trimad was found to be more potent than ascorbic acid at all given concentrations. It also showed significant ferric reducing potential (0.110% at 20μg/ml) and ABTS scavenging activity (31% at 80μg/ml). Trimad however showed very minimal (4.754% at 100μg/ml) lipid peroxidation activity. Our findings indicate promising antioxidant activity of Trimad extract however it needs further attention for its effective use in both modern and traditional system of medicines

Mind the Gap! A Journey towards Computational Toxicology

“…STEP is an open platform to share data and informationf or bettera ddressing paediatric drug development. [75,76] An umber of commercial databases, whose appeal is sometimes in the promise of an expanded chemical space, are also available for the purpose of predictive toxicology.F or instance,t he Vitic database [77,78] is ap ilot toxicology repository suitable as as ourceo ft oxicological information (comprising data about genotoxicity,c arcinogenicity,h epatotoxicity,h ERG, and skin sensitization) and to support QSAR studies.V itic is searchable via chemical structures and sub-structural fragments as well as toxicology and physicochemical data. Notably,V itic has also been used as the reference repository within the Innovative Med-icines Initiative( IMI) eTox project,i ncluding industrial and academic partners, to developi nnovative strategies for the prediction of the safety and the side-effects of new candidate medicines.…”