In vitro gene delivery mediated by chitosan. Effect of pH, serum, and molecular mass of chitosan on the transfection efficiency

Sato, Toshinori; Ishii, Tsuyoshi; Okahata, Yoshio

doi:10.1016/s0142-9612(00)00385-9

Cited by 402 publications

(225 citation statements)

References 22 publications

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“…Lipofectamine reduces significantly the cell viability (Corsi et al, 2003;Sato et al, 2001;Thanou et al, 2002). In our previous study (Santos-Carballal et al, 2015), we evaluated the cytotoxity of CS-microRNA complexes of varying N/P ratio formed by CS of different Mw and DA after 6 and 24 h. No differences were observed between the two elapsed times.…”

Section: Discussionmentioning

confidence: 99%

Chitosan as a non-viral co-transfection system in a cystic fibrosis cell line

Fernández

Santos-Carballal

Weber

et al. 2016

International Journal of Pharmaceutics

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Successful gene therapy requires the development of suitable vehicles for the selective and efficient delivery of genes to specific target cells at the expense of minimal toxicity. In this work, we investigated a non-viral gene delivery system based on chitosan (CS) to specifically address cystic fibrosis (CF). Thus, electrostatic self-assembled CS-pEGFP and CS-pEGFPsiRNA complexes were prepared from high-pure fully characterized CS (Mw ~20 kDa and degree of acetylation ~30%). The average diameter of positively-charged complexes (i.e.  ~ +25 mV) was ~200 nm. The complexes were found relatively stable over 14 h in OptiMEM. Cell viability study did not show any significant cytotoxic effect of the CS-based complexes in a human bronchial cystic fibrosis cell line (s). We evaluated the transfection efficiency of this cell line with both CS-pEGFP and co-transfected with CS-pEGFP-siRNA complexes at (N/P) charge ratio of 12. We reported an increase in the fluorescence intensity of CS-pEGFP and a reduction in the cells co-transfected with CS-pEGFP-siRNA. This study shows proof-of-principle that co-transfection with chitosan might be an effective delivery system in a human CF cell line. It also offers a potential alternative to further develop therapeutic strategies for inherited disease treatments, such as CF.

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Section: Discussionmentioning

confidence: 99%

Chitosan as a non-viral co-transfection system in a cystic fibrosis cell line

Fernández

Santos-Carballal

Weber

et al. 2016

International Journal of Pharmaceutics

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show abstract

“…'Gold standard' vectors include Lipofectamine 2000™ and polyethyleneimine (PEI) which can achieve high transfection efficiencies in many cell types, but can also cause cytotoxicity [14,15,44]. Chitosanis known to be non-toxic and good transfection efficiency has been reported in some cell lines [16,25,26,45]. However, in this study, the cell type of interest was the mesenchymal stem cell (MSCs), a cell type of greater clinical significance in tissue engineering applications due to its ability to differentiate into a number of mesoderm-derived cell types, but which is notoriously difficult to transfect [46].…”

Section: Discussionmentioning

confidence: 99%

Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds

Raftery

Tierney

Curtin

et al. 2015

Journal of Controlled Release

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“…Moreover, the transfection efficiency was 2 to 3 times higher in the presence of serum than that in the absence of serum. The enhancement with serum may be caused by the cell function raised by the addition of serum since major components in serum like albumin and globulin have little effect on the transfection efficiency of chitosan-based polyplexes (Sato et al, 2002).…”

Section: Chitosanmentioning

confidence: 99%