In vitro hemocompatibility and cytocompatibility of dexamethasone-eluting PLGA stent coatings

Zhang, Jiang; Liu, Yang; Luo, Rifang; Chen, Si; Li, Xin; Yuan, Shuheng; Wang, Jin; Huang, Nan

doi:10.1016/j.apsusc.2014.12.002

Cited by 17 publications

(7 citation statements)

References 49 publications

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“…The characteristic absorbance wavelengths obtained for DEX, PLGA fibers and DEX-loaded PLGA fibers are in good agreement with those previously reported in literature. [56][57][58] We hypothesized that the alignment of the fibers would have an effect on the release of encapsulated DEX. To assess this hypothesis, first the structural and thermal properties of both random and aligned fibers were analyzed using XRD and DSC techniques, respectively.…”

Section: Conical Ratio = D 2 /D 1 (Equation 1)mentioning

confidence: 99%

Electrospinning of highly aligned fibers for drug delivery applications

Eslamian

Khorrami

et al. 2019

J. Mater. Chem. B

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Section: Conical Ratio = D 2 /D 1 (Equation 1)mentioning

confidence: 99%

Electrospinning of highly aligned fibers for drug delivery applications

Eslamian

Khorrami

et al. 2019

J. Mater. Chem. B

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“…In contrast, an increase in FWHM (broad peak) of the 1725–1750 cm –1 peak was observed in the case of 502H samples stored at 40 °C/75%, which could be due to the uniform distribution of PLGA over the DM crystal. − In addition, the increase in FWHM could also be due to the formation of heterogeneous structures of degraded PLGA products. The heterogeneous structures, that is, with different chain lengths, could be due to the continuous diffusion of degraded products into the media limiting the abrupt autocatalytic degradation.…”

Section: Resultsmentioning

confidence: 92%

Influence of PLGA End Groups on the Release Profile of Dexamethasone from Ocular Implants

et al. 2023

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The present study deals with the development of dexamethasone (DM)-loaded implants using ester end-capped Resomer RG 502 poly(lactic acid-co-glycolic acid) (PLGA) (502), acid endcapped Resomer RG 502H PLGA (502H), and a 502H:502 mixture (3:1) via hot melt extrusion (HME). The prepared intravitreal implants (20 and 40% DM loaded in each PLGA) were thoroughly investigated to determine the effect of different end-capped PLGA and drug loading on the long-term release profile of DM. The implants were characterized for solid-state active pharmaceutical ingredient (APIs) using DSC and SWAXS, water uptake during stability study, the crystal size of API in the implant matrix using hot-stage polarized light microscopy, and in vitro release profile. The kinetics of PLGA release was thoroughly investigated using quantitative 1 H NMR spectroscopy. The polymorph of DM crystal was found to remain unchanged after the extrusion and stability study. However, around 3 times reduction in API particle size was observed after the HME process. The morphology and content uniformity of the RT-stored samples were found to be comparable to the initial implant samples. Interestingly, the samples (mainly 502H) stored at 40 °C and 75% RH for 30 d demonstrated marked deformation and a change in content uniformity. The rate of DM release was higher in the case of 502H samples with a higher drug loading (40% w/w). Furthermore, a simple digital in vitro DM release profile derived for the formulation containing a 3:1 ratio of 502H and 502 was comparable with the experimental release profile of the respective polymer mixture formulation. The temporal development of pores and/or voids in the course of drug dissolution, evaluated using μCT, was found to be a precursor for the PLGA release. Overall, the release profile of DM was found to be dependent on the PLGA type (independent of subtle changes in the formulation mass and diameter). However, the extent of release was found to be dependent on DM loading. Thus, the present investigation led to a thorough understanding of the physicochemical properties of different end-capped PLGAs and the underlying formulation microstructure on the release profile of a crystalline water-insoluble drug, DM, from the PLGA-based implant.

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“…Figure 2(b) shows the different spectrometry of DEX, GnP, GOD, DNF, GnP-NF-GOD (-), and GnP-NF. The DEX adsorption peak was shown in 3481 cm −1 , 1649 cm −1 , and 1045 cm −1 , which can be attributed to O-H, C=C, and C-F stretch vibration [46]. Similarly, the DNF sample peaked, confirming that DEX was successfully incorporated into PCL/CS.…”

Section: Chemical Analysis Of Nanofibersmentioning

confidence: 79%

Preparation and characterization of a biocompatible glucose-sensitive electrospun nanofibers scaffolds containing dexamethasone with enhanced osteogenic properties in vitro high glucose environment

Jia¹,

Liu

Tan

et al. 2023

Biomed. Mater.

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Diabetes has made it challenging to repair alveolar bone defects. A successful method for bone repair utilizes a glucose-sensitive osteogenic drug delivery. This study created a new glucose-sensitive nanofiber scaffold with controlled dexamethasone (DEX) release. DEX-loaded polycaprolactone/chitosan (PCL/CS) nanofibers scaffolds were created using electrospinning. The nanofibers had high porosity (>90%) and proper drug loading efficiency (85.51 ± 1.21%). Then, glucose oxidase (GOD) was immobilized on the obtained scaffolds by a natural biological cross-linking agent, genipin (GnP), after soaking in the mixture solution containing GOD and GnP. The enzyme properties and glucose sensitivity of the nanofibers were investigated. The results showed that GOD was immobilized on the nanofibers and exhibited good enzyme activity and stability. Meanwhile, the nanofibers expanded gradually in response to the increase in glucose concentration, followed by the release of DEX increased. The phenomena indicated that the nanofibers could sense glucose fluctuation and possess favorable glucose sensitivity. In addition, the genipin nanofibers group showed lower cytotoxicity in the biocompatibility test compared with a traditional chemical cross-linking agent. Lastly, the associated osteogenesis evaluation found that the scaffolds effectively promoted MC3T3-E1 cells' osteogenic differentiation in high-glucose environments. As a result, the glucose-sensitive nanofibers scaffolds offer a viable treatment option for people with diabetes with alveolar bone defects.

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In vitro hemocompatibility and cytocompatibility of dexamethasone-eluting PLGA stent coatings

Cited by 17 publications

References 49 publications

Electrospinning of highly aligned fibers for drug delivery applications

Electrospinning of highly aligned fibers for drug delivery applications

Influence of PLGA End Groups on the Release Profile of Dexamethasone from Ocular Implants

Preparation and characterization of a biocompatible glucose-sensitive electrospun nanofibers scaffolds containing dexamethasone with enhanced osteogenic properties in vitro high glucose environment

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