In vitro HIV type 1 reverse transcriptase inhibitory activities of Thai medicinal plants and Canna indica L. rhizomes

Woradulayapinij, Warunya; Soonthornchareonnon, Noppamas; Wiwat, Chanpen

doi:10.1016/j.jep.2005.03.030

Cited by 123 publications

(74 citation statements)

References 12 publications

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“…A plant extract is considered active on the reverse transcriptase, if the concentration inhibiting 50% enzyme activity (IC 50 ) is below 200 µg/ml (Woradulayapinij et al 2005). In this work, extracts from B. engleriana exerted such ant-RT activity (Table 4) …”

Section: Resultsmentioning

confidence: 95%

“…In order to avoid the tannins interference, bovine serum albumin (Fraction V) was added to assay buffers to a final assay concentration of 0.2%(w/v) to adsorb possible tannins from crude extract (Harnett et al, 2005). Extracts were first tested at 0.2 mg/ml and samples which reduced activity by at least 50% were considered active (Woradulayapinij et al, 2005). Twofold dilutions (3.13-100 μg/ml) were then made in order to determine IC 50 values.…”

Section: Anti-hiv Investigations: Reverse Transcriptase (Rt) Assaymentioning

confidence: 99%

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Evaluation of four Cameroonian medicinal plants for anticancer, antigonorrheal and antireverse transcriptase activities

Mbaveng¹,

Kuete²,

Mapunya³

et al. 2011

Environmental Toxicology and Pharmacology

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Methanol extracts from the leaves, bark and roots of four Cameroonian medicinal plants, Bersama engleriana, Cupressus lusitanica, Vitellaria paradoxa and Guibourtia tessmannii were tested for their in vitro cytotoxicity, antigonorrheal and antireverse transcriptase activities. The XTT (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt) assay, the dilution method and reverse transcriptase (RT) assay were used for the investigations. Preliminary phytochemical analysis of the extracts was also conducted using standrad methods. Results showed that all extracts contained compounds belonging to the classes of phenols and terpenoids. They were also able to reduce in dose dependent manner, the proliferation of the cancer THP-1, DU145, HeLa, MCF-7, HepG2 and the normal Vero cells. IC 50 values below 30 µg/ml were noted with extract from the three parts of B. engleriana on at least two of the five studied cancer cell lines, the lowest value of 5.9 µg/ml being obtained with sample from the bark. IC 50 values below 30 µg/ml were also recorded with extracts from the leaves (on HeLa cells) and bark (on MCF-7) of G. tessmanii, and that from the bark of C. lusitanica on MCF-7. Extracts from B. engleriana and those from 2 the bark of V. paradoxa gave the minimal inhibitory concentrations (MIC) values below 100 μg/ml on most of the ten tested Nesseria gonorrhoeae strains. Extracts from B. engleriana also inhibited more than 80% the activity of the Human Immuno-deficiency Virus (HIV) enzyme. Finally, the results of the present study provide baseline information for the use of B. engleriana, C. lusitanica, G. tessmanii, V. paradoxa.

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Section: Resultsmentioning

confidence: 95%

Section: Anti-hiv Investigations: Reverse Transcriptase (Rt) Assaymentioning

confidence: 99%

Evaluation of four Cameroonian medicinal plants for anticancer, antigonorrheal and antireverse transcriptase activities

Mbaveng¹,

Kuete²,

Mapunya³

et al. 2011

Environmental Toxicology and Pharmacology

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show abstract

“…The leaves of this plant have been reported for anti-angiogenic [1], antioxidant [2], antibactericidal [3], antifungal [4], anti-arthritic [5], anti-inflammatory and antinociceptive [6], antisickling [7], anthelmintic [8], cytotoxicity [9], larvicidal, and adulticidal activities [10]. In addition, its aerial parts showed in vitro HIV type 1 reverse transcriptase inhibitor [11], antiinflammatory and analgesic [12], sedative, and hypnotic activities [13]. This plant has also been used by people in Papua as a male contraceptive [14].…”

Section: Introductionmentioning

confidence: 99%

Metabolite Profiling of Justicia gendarussa Burm. f. Leaves Using UPLC-UHR-QTOF-MS

Ningsih

2015

Sci. Pharm.

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An ultra-performance liquid chromatography ultra-high-resolution quadrupoletime-of-flight-mass spectrometry (UPLC-UHR-QTOF-MS) metabolite profiling of Justicia gendarussa Burm. f. leaves was performed. PCA and HCA analyses were applied to observe the clustering patterns and inter-sample relationships. It seemed that the concentrations of Ca, P, and Cu in the soil could affect the metabolite profiles of Justicia gendarussa. Six significant metabolites were proposed.

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“…The aerial parts of this plant are useful in the treatment of diabetes (Pullaiah and Naidu 2003). Besides, the plant also possesses larvicidal, wound healing, anti HIV, anticancer, trypanocidal, antibacterial and antipyretic activities (Li et al 2005;Woradulayapinij et al 2005;Kannathasan et al 2007;Manjunatha et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Rapid in vitro propagation system through shoot tip cultures of Vitex trifolia L.–an important multipurpose plant of the Pacific traditional Medicine

Ahmed

Anis

2014

Physiol Mol Biol Plants

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A rapid and efficient plant propagation system through shoot tip explants was established in Vitex trifolia L., a medicinally important plant belonging to the family Verbenaceae. Multiple shoots were induced directly on Murashige and Skoog (MS) medium consisting of different cytokinins, 6-benzyladenine (BA), kinetin (Kin) and 2-isopentenyl adenine (2-iP), BA at an optimal concentration of 5.0 μM was most effective in inducing multiple shoots where 90 % explants responded with an average shoot number (4.4 ±0.1) and shoot length (2.0±0.1 cm) after 6 weeks of culture. Inclusion of NAA in the culture medium along with the optimum concentration of BA promoted a higher rate of shoot multiplication and length of the shoot, where 19.2±0.3 well-grown healthy shoots with an average shoot length of 4.4±0.1 cm were obtained on completion of 12 weeks culture period. Ex vitro rooting was achieved best directly in soilrite when basal portion of the shoots were treated with 500 μM indole-3-butyric acid for 15 min which was the most effective in inducing roots, as 95 % of the microshoots produced roots. Plantlets went through a hardening phase in a controlled plant growth chamber, prior to ex-vitro transfer. Micropropagated plants grew well, attained maturity and flowered with 92 % survival rate. The results of this study provide the first report on in vitro plant regeneration of Vitex trifolia L. using shoot tip explants.

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In vitro HIV type 1 reverse transcriptase inhibitory activities of Thai medicinal plants and Canna indica L. rhizomes

Cited by 123 publications

References 12 publications

Evaluation of four Cameroonian medicinal plants for anticancer, antigonorrheal and antireverse transcriptase activities

Evaluation of four Cameroonian medicinal plants for anticancer, antigonorrheal and antireverse transcriptase activities

Metabolite Profiling of Justicia gendarussa Burm. f. Leaves Using UPLC-UHR-QTOF-MS

Rapid in vitro propagation system through shoot tip cultures of Vitex trifolia L.–an important multipurpose plant of the Pacific traditional Medicine

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