In vitro host immune response against Acinetobacter baumannii infection using A549 cell line

Mea, Hing Jian; Madhavan, Prakash; Palanisamy, Navindra Kumari; Yong, Peter; Wong, Eng Hwa

doi:10.1016/j.ijid.2020.09.352

Cited by 2 publications

(1 citation statement)

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“…Interestingly, membrane damage activity of LASP-132 was decreased at 24 h; in particular, no distinct population of PI-positive cells was observed in A. baumannii 5075R, possibly due to the remodelling of the bacterial outer membrane at 24 h ( Zhu et al, 2020 ). We also investigated the in vitro toxicity of LASP-072, LASP-097, and LASP-132 in human lung epithelial A549 cells ( Figure 6 ) ( Ahmed et al, 2017 ; Mea et al, 2020 ). Interestingly, the antibacterial LASP-072 and LASP-132 caused <10% death of A549 cells and no significant difference was observed with the untreated control samples.…”

Section: Resultsmentioning

confidence: 99%

An Efficient Approach for the Design and Synthesis of Antimicrobial Peptide-Peptide Nucleic Acid Conjugates

Patil

Thombare

et al. 2022

Front. Chem.

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Peptide-Peptide Nucleic Acid (PNA) conjugates targeting essential bacterial genes have shown significant potential in developing novel antisense antimicrobials. The majority of efforts in this area are focused on identifying different PNA targets and the selection of peptides to deliver the peptide-PNA conjugates to Gram-negative bacteria. Notably, the selection of a linkage strategy to form peptide-PNA conjugate plays an important role in the effective delivery of PNAs. Recently, a unique Cysteine- 2-Cyanoisonicotinamide (Cys-CINA) click chemistry has been employed for the synthesis of cyclic peptides. Considering the high selectivity of this chemistry, we investigated the efficiency of Cys-CINA conjugation to synthesize novel antimicrobial peptide-PNA conjugates. The PNA targeting acyl carrier protein gene (acpP), when conjugated to the membrane-active antimicrobial peptides (polymyxin), showed improvement in antimicrobial activity against multidrug-resistant Gram-negative Acinetobacter baumannii. Thus, indicating that the Cys-CINA conjugation is an effective strategy to link the antisense oligonucleotides with antimicrobial peptides. Therefore, the Cys-CINA conjugation opens an exciting prospect for antimicrobial drug development.

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Section: Resultsmentioning

confidence: 99%