In vitro human gastro-intestinal enzyme digestibility of globulin isolate from oil palm (Elaeis guineensis var. tenera) kernel meal and the bioactivity of the digest

Tapal, Arun; Vegarud, Gerd Elisabeth; Sreedhara, Ashoka; Hegde, Prajna; Tiku, Purnima Kaul

doi:10.1039/c5ra25232f

Cited by 20 publications

(14 citation statements)

References 38 publications

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“…These results showed that PIs are not resistant to digestive proteases. Similar results were reported for other digests of plant proteins obtained from almond (Sze-Tao and Sathe, 2000) and oil palm kernel (Tapal et al, 2016)…”

Section: Digestibility Of Protein Isolatesupporting

confidence: 90%

Plum oil cake protein isolate: A potential source of bioactive peptides

Čakarević¹,

Vidović²,

Vladić³

et al. 2019

Food & Feed Res

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In this study some functional properties and the in vitro digestibility of protein isolates from plum oil cakes, obtained by supercritical fluid extraction and cold pressing, as a control, were described and compared. Amygdalin contents in the protein isolates were at amounts considerably lower than regulatory. Solubility profiles of both protein isolates were typical for this type of plant proteins. Proteins showed good digestibility, which was assessed by gastrointestinal proteases (pepsin and pancreatin). SDS-PAGE analysis was used for characterisation of protein digests which showed that the protein isolates were completely digested. In vitro antioxidant capacity by three complementary methods and enzyme inhibitory effects towards Angiotensin-I Converting Enzyme (ACE) related to the onset of hypertension were determined. All obtained protein hydrolysates acted as DPPH and ABTS scavengers, as reducing agents and also as ACE enzyme inhibitor. Hence, the protein isolates obtained from plum kernel cake showed to be a potential source of natural products for food applications, with good digestibility and beneficial bioactive properties.

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Section: Digestibility Of Protein Isolatesupporting

confidence: 90%

Plum oil cake protein isolate: A potential source of bioactive peptides

Čakarević¹,

Vidović²,

Vladić³

et al. 2019

Food & Feed Res

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“…The study by Tapal et al . () describes the ex vivo digestibility of globulin isolate prepared from oil palm kernel. The hydrolysate, obtained by in vitro human gastrointestinal enzyme digestibility of the globulin isolate, exhibited potent ACE inhibitory activity (IC 50 = 50 μg mL −1 ) and anticancer activity against human colon epithelial cancer HT‐29 cells and hepatocarcinoma HepG2 cells (Tapal et al ., ).…”

Section: Introductionmentioning

confidence: 99%

“…() describes the ex vivo digestibility of globulin isolate prepared from oil palm kernel. The hydrolysate, obtained by in vitro human gastrointestinal enzyme digestibility of the globulin isolate, exhibited potent ACE inhibitory activity (IC 50 = 50 μg mL −1 ) and anticancer activity against human colon epithelial cancer HT‐29 cells and hepatocarcinoma HepG2 cells (Tapal et al ., ). Another important group of peptides are anti‐inflammatory peptides, as the inflammatory process is fundamental to a number of diseases, for example cancers or cardiovascular disease (Chakrabarti et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Digestion and bioavailability of bioactive phytochemicals

Karaś

Jakubczyk

Szymanowska

et al. 2016

Int J of Food Sci Tech

136

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There are many scientific reports on determination of the content and biological activity of compounds found in food. However, these analyses are not sufficient to determine their effect on the human body. During digestion of food ingredients, many changes can modify their structure and this may affect their absorption and bioactivity. Many phenolic aglycones are hydrophilic and can be absorbed through biological membranes by diffusion. However, most polyphenols occur in the glycosidic form, which undoubtedly affects their absorption in the intestine. Oligopeptides are also absorbable via secondary active transport but based on the hydrogen ion gradient or with transporter PepT1. The bioavailability of phytochemicals is determined by their molecular weight or chemical structure and the food matrix. Accordingly, the aim of this work was to present the novel scientific reports related to the influence the many factors on digestibility, bioaccessibility and activity of selected bioactive compounds of plant origin.

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“…19 However, both ADVFNPR and LVYIIQGR exhibited higher antioxidant activity than the smaller peptides from rice residue (464.2 Da, IC 50 : 43.55 mg mL À1 ), 22 tilapia protein (317.3 Da, IC 50 : 26.4 mg mL À1 ) 6 and walnut protein (423.23 Da, IC 50 : 310.0 mg mL À1 ), 3 indicating that antioxidant activity of peptides was not dependent only on M w . Moreover, Tapal et al 12 also obtained the peptide ADVFNPR from palm kernel globulin and found that it had antihypertensive and anticancer activity. It was demonstrated that antioxidant activity of peptides is positively correlated with the hydrophobic property.…”

Section: Characterization Of Puried Peptidesmentioning

confidence: 99%

“…10 It was found that enzymatic hydrolysates of the globulin showed anticancer, antihypertensive and antibacterial activities; also several peptides obtained from the glutelin-2 could effectively lower the systolic blood pressure of spontaneously hypertensive rats. [11][12][13] Dilute acid-soluble seed storage proteins are commonly known as glutelin-1 proteins. 14 A previous study has demonstrated that palm kernel expeller glutelin-1 (PKEG-1) exhibited high scavenging activity of DPPH radical (77.69%), ABTS radical (64.19%), superoxide radical (49.54%) and hydroxyl radical (34.41%); also it exhibited high reducing power (0.211 AE 0.009), 10 indicating that antioxidant peptides could be isolated from it.…”

Section: Introductionmentioning

confidence: 99%