In vitro immunomodulating properties of selected Sudanese medicinal plants

Koko, Waleed S.; Mesaik, M. Ahmed; Yousaf, Sammer; Galal, M.; Choudhary, M. Iqbal

doi:10.1016/j.jep.2008.03.007

Cited by 73 publications

(58 citation statements)

References 29 publications

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“…Cell viability was determined using the standard Trypan blue dye exclusion method described previously (Koko et al, 2008). In brief, PMNs (1 Â 10 6 ) suspended in 1 mL of HBSS supplemented with 2% FCS was incubated with 10 mL of either tested samples (final concentrations ranging from 1 to 1000 mM) or DMSO for 30 min at 37 C. PMNs viability was determined by adding each sample to 112 mL of 0.4% Trypan blue and scoring the first 100 cells encountered in the field of view of a light microscope as either alive or dead based on the uptake of Trypan blue.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Inhibitory effects of phenolic compounds fromArtocarpus styracifoliuson respiratory burst of rat neutrophils

Ren

Xiang²,

Hu³

et al. 2014

Pharmaceutical Biology

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Materials and methods:The anti-respiratory burst activities of eight phenolics (20 mM) were assessed by determining luminol-dependent chemiluminiscence in rat PMNs. Cytotoxicity of active compounds (1-1000 mM) was assayed by Trypan blue dye exclusion method. Cell-free models were employed to evaluate scavenging capacity of active compounds (20 mM) against reactive oxygen species.Results: The PMA-induced respiratory burst was significantly inhibited (p50.05) by six isoprenylated phenolics (AS1-6) at the concentration of 20 mM (below the toxic concentration) with the inhibition rate ranging from 25.0 to 99.6%. The inhibitory potency estimated by IC 50 was in the order of AS1 (3.1 mM) 4AS6 (5.9 mM) 4AS2 (9.1 mM) 4AS3 (10.0 mM) 4AS5 (29.7 mM) 4AS4 (57.7 mM). AS1-4, four isoprenylated flavones, potently quenched superoxide anion, hydroxyl radical, and hydrogen peroxide at the concentration of 20 mM with their scavenging rates in the range of 30.1-78.1%, 35.4-69.7%, and 65.5-86.3%, respectively. In contrast, AS5-6, two isoprenylated 2-arylbenzofurans, showed less effect than that exhibited by AS1-4. Conclusion and discussion: The isoprenylated phenolics from A. styracifolius can potently inhibit PMA-induced respiratory burst in rat neutrophils without showing cytotoxicity. The inhibitory effects of these isoprenylated phenolics on the respiratory burst might depend on their different types of structure.

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Section: Cytotoxicity Assaymentioning

confidence: 99%

Inhibitory effects of phenolic compounds fromArtocarpus styracifoliuson respiratory burst of rat neutrophils

Ren

Xiang²,

Hu³

et al. 2014

Pharmaceutical Biology

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“…[24][25][26][27] Immunomodulatory properties of Sudanese medicinal plants have also been observed. 28 In this review, we provide an updated overview of the most important plants used in Sudanese traditional medicines.…”

Section: Traditional Sudanese Medicinementioning

confidence: 99%

Gems from traditional north-African medicine: medicinal and aromatic plants from Sudan

Khalid

Abdalla

Abdelgadir

et al. 2012

Nat. Prod. Bioprospect.

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Sudanese folk medicine represents a unique blend of indigenous cultures with Islamic, Arabic and African traditions. In addition, Sudan encompasses different terrains and climatic zones, ranging from desert and semi-desert in the north to equatorial with a short rainy season (semi-aridand semi-humid) in the centre to equatorial with a long rainy season (arid-humid and equatorialhumid) in the south. This variation contributes to the immense diversity of vegetation in the region. The flora of Sudan consists of 3137 species of flowering plants belonging to 170 families and 1280 genera. It is estimated that 15% of these plants are endemic to Sudan. The intersection of diverse cultures and the unique geography holds great potential for Sudanese herbal medicine. Medicinal and aromatic plants and their derivatives represent an integral part of life in Sudan. Indigenous remedies are the only form of therapy available to the majority of poor people. It has been estimated that only 11% of the population has access to formal health care. Therefore, research on the desired pharmacological effects and possible unwanted side effects or toxicity is required to improve efficacy and safety of Sudanese herbal medicine. In the future, it would be preferable to promote the use of traditional herbal remedies by conversion of raw plant material into more sophisticated products instead of completely replacing the traditional remedies with synthetic products from industrialized countries. The present review gives an overview of traditional Sudanese medicinal and aromatic herbs and their habitats, traditional uses, and phytochemical constituents.

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“…In Sudanese traditional medicine, G. alata is being used against epilepsy, as an antispasmodic (El Ghazali et al 1994, or to treat cough and intestinal complaints (Hostettmann & Marston 2002, EL-Kamali & EL-amir 2010. In a recent study, G. alata showed strong immunomodulation inhibitory activity and cell oxidative burst response (Koko et al 2008). G. alata root is frequently used for the management of diabetes in Sudan (EL-Kamali 2009, EL-Kamali & EL-amir 2010.…”

Section: Introductionmentioning

confidence: 99%

The antidiabetic effect of Geigeria alata is mediated by enhanced insulin secretion, modulation of β-cell function, and improvement of antioxidant activity in streptozotocin-induced diabetic rats

Hafizur¹,

Babiker²,

Yagi³

et al. 2012

Journal of Endocrinology

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In Sudanese folk medicine, Geigeria alata roots have been used for the management of diabetes for a long time. However, its antidiabetic activity is unreported. In this study, G. alata methanolic extract was tested for its antidiabetic, antioxidant, and b-cell modulatory effects in a streptozotocin-induced diabetic rat model. In this model of diabetic rats, the oral glucose tolerance test with G. alata extract at 125, 250, and 500 mg/kg doses revealed the efficacy of the 250 mg/kg dose in improving glucose tolerance comparable to the standard drug glibenclamide. Diabetic rats were treated with a 250 mg/kg dose of G. alata extract orally for 2 h (acute) or 14 days (chronic). In the case of acute treatment, the extract lowered blood glucose levels significantly at 120 min both in nondiabetic and diabetic rats. Chronic treatment of diabetic rats with 250 mg/kg of G. alata extract resulted in a significant decrease in blood glucose level closer to that of nondiabetic rats. Interestingly, increased serum insulin, improved b-cell function, and antioxidant status were observed in G. alata-treated diabetic rats. G. alata also showed strong antioxidant and a-glucosidase inhibitory activities in in vitro assays. These data show direct evidence that G. alata has antidiabetic activity and suggest that the antidiabetic activity is due to enhanced insulin secretion, modulation of b-cell function, and improvement of antioxidant status.

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In vitro immunomodulating properties of selected Sudanese medicinal plants

Cited by 73 publications

References 29 publications

Inhibitory effects of phenolic compounds fromArtocarpus styracifoliuson respiratory burst of rat neutrophils

Inhibitory effects of phenolic compounds fromArtocarpus styracifoliuson respiratory burst of rat neutrophils

Gems from traditional north-African medicine: medicinal and aromatic plants from Sudan

The antidiabetic effect of Geigeria alata is mediated by enhanced insulin secretion, modulation of β-cell function, and improvement of antioxidant activity in streptozotocin-induced diabetic rats

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