In vitro induction and generation of tetraploid plants of Sophora tonkinensis Gapnep

Abstract

Background:Sophora tonkinensis Gapnep. is an important medical plant in China. Early researches of S. tonkinensis were focused on rapid propagation and quality analysis of in vitro tissue culture plantlet, and still no research focuses on the plant breeding of and there were no excellent varieties for artificial cultivation of S. tonkinensis.Objective:To set up a method to generate and select the best varieties of S. tonkinensis by polyploid breeding after induction by colchicine treatment.Materials and Methods:The adventitious buds were submerged in different concentrations of aqueous colchicine solution for different lengths of time to induce polyploidy in the plants, and the induced buds were identified by root-tip chromosome determination and leaf characteristics comparison. The contents of matrine and oxymatrine of radix ex rhizoma in 13 selected tetraploid lines were collected after 90 days in vitro rooting culture and were evaluated to provide evidence of good qualities of tetraploid S. tonkinensis.Results:The results showed that the highest percentage of tetraploid induction was 23.33% and occurred in the 0.2% (w/v) colchicine treatment for 30 h. Fifty lines of tetraploid plants were obtained and 12 of the 13 selected tetraploid lines exhibited higher productivity of total contents of matrine and oxymatrine when compared to controls.Conclusion:The data demonstrate that polyploidy induction can be beneficial for improving the medicinal value of S. tonkinensis.SUMMARY Colchicine has a good in vitro induction effect on the tetraploid plants of Sophora tonkinensisThe leaf indices and stomatal apparatus of tetraploid plants were slightly larger than diploid plantsThe total content of matrine and oxymatrine of some tetraploid lines for 90-day-old in vitro rooting culture was higher than the diploid. Abbreviations used: MS medium: Murashige and Skoog medium; BAP: 6-benzylaminopurine; NAA: A-naphthaleneacetic acid; IAA: Indole-3-acetic acid; KT: Kinetin; IBA: Indole-3-butyric acid; ABT: Rooting power.

Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba

“…These changes may provide polyploids with short-term adaptive potential (Van de Peer et al, 2017) and the opportunity to exploit new niches (e.g., Marchant et al, 2016). In addition, synthetic polyploids have been largely employed to increase agronomic traits mainly due to its higher physiological and morphological fitness compared to their natural diploids (Chung et al, 2017;Cui et al, 2017;Salma et al, 2017;Wei et al, 2018).…”

In vitro induction and characterisation of tetraploid drumstick tree (Moringa oleifera Lam.)

“…And, most of the tetraploid Sophora tonkinensis Gapnep. lines exhibited higher productivity of total contents of matrine and oxymatrine than diploids [ 35 ]. In addition, tetraploid E. purpurea had higher caffeic acid derivative and alkamide contents [ 8 ].…”

Polyploidy Induction in <i>Salvia officinalis</i> L. and Its Effects on Some Morphological and Physiological Characteristics

“…The morphological evaluation of plants is a suitable approach for the initial selection of polyploids. Plant s descriptive features constantly change with increasing chromosome numbers recognized as a gigas effect (Wei et al 2018). If these changes happen in the commercial part of the plant as in medicinal and vegetable plants, it might become a valuable feature for plant improvement.…”

“…Also, it was reported that the most successful way to induce polyploidy is through seed treatment; as seed treatment with colchicine before germination produces more tetraploid plants rather than germinated ones (Lehrer et al 2008). Successful induction of polyploidy using colchicine was reported in some plants such as sage (S. polyploids L.) (Kobayashi et al 2008), parsley (Petroselinum crispum L.) (Nasirvand et al 2018), Sophora tonkinensis L. (Wei et al 2018) and cabbage (Brassica oleracea L.) (Albertin et al 2005).…”