In vitro interaction of certain antimicrobial agents in combination with plant extracts against some pathogenic bacterial strains

Rakholiya, Kalpna; Chanda, Sumitra

doi:10.1016/s2221-1691(12)60327-x

Cited by 50 publications

(29 citation statements)

References 15 publications

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“…In explanation, antibiotics have played important role to treat various bacterial infections since their time of introduction, but wide use of antibiotics has led to development of resistant strains, which is becoming a global problem. There is requirement to find some alternatives to tackle the problem of emergence of drug resistant microorganisms (Rakholiya and Chanda, 2012).…”

Section: Resultsmentioning

confidence: 99%

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Synergistic Effect of Combined Antibiotic and Methanol Extract of Eucalyptus camaldulensis leaf Against Staphylococcus aureus and Pseudomonas aeruginosa

Reda

El-Zawahry

Omar

2017

Int J Appl Sci Biotechnol

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A total of seventy-five medical specimens were collected from different patients admitted to Zagazig university hospital, Egypt. The selected bacterial isolates were distributed as 45 Gram negative bacterial isolates and 30 Gram positive bacterial isolates. The most effective antibiotic was gentamycin followed by amikacin and nitrofurantoin. The multi-drug resistant (MDR) bacterial isolates were selected and identified to four groups; Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus. The selected isolates were screened for their capability of biofilm, hemolysins, lecithinase and protease production. S. aureus AM23 and P. aeruginosa AM41 were selected as the highest biofilm and degrading enzymes producers. Furthermore, twenty-four methanolic and aqueous crude extracts derived from different medicinal plants in Egypt were screened for their antibacterial activity against both selected MDR strains. Methanolic extract of Eucalyptus camaldulensis leaf showed the greatest effect on the tested bacteria. Also, there was a synergistic effect of combined E. camaldulensis leaf extract and antibiotics (Gentamycin and Ceftriaxone) against tested organisms. Transmission electron microscopy showed changes in cell shapes, size, contents and cell envelop of antimicrobials treated bacterial cells compared to the control. Our findings proved that the leaf extracts of E. camaldulensis had great potential as antimicrobial agents in the treatment of infectious organisms.

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Section: Resultsmentioning

confidence: 99%

“…Indifference occurs when the effect of two drugs together is the same of either alone. Antagonism occurs when the effect of two drugs together is less than the effect of both alone (Rakholiya and Chanda, 2012).…”

Section: Combination Of Gentamycin and Ceftriaxone With E Camaldulenmentioning

confidence: 99%

Synergistic Effect of Combined Antibiotic and Methanol Extract of Eucalyptus camaldulensis leaf Against Staphylococcus aureus and Pseudomonas aeruginosa

Reda

El-Zawahry

Omar

2017

Int J Appl Sci Biotechnol

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“…The petri-dishes were prepared by pouring 20 ml of molten Muller Hinton Agar (MHA) in biological safety cabinet class-2 type to avoid extraneous contamination. The MHA was seeded with 200 μl test culture containing 1 × 10 8 cfu/ml as McFarland 0.5 turbidity standard [24].…”

Section: Methodsmentioning

confidence: 99%

“…The emergence of multi-drug resistant bacteria and the infectious diseases caused by them is a serious worldwide problem [14]. Therefore combination therapy of antibiotics with natural antibiotics is profitable for patients with serious infections caused by highly resistant bacteria.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Anti-Microbial Agents with Ascorbic Acid in Catheter Associated Urinary Tract Infection

Afzal¹,

Ashraf²,

Bukhsh³

et al. 2017

J Infect Dis Preve Med

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“…Interest for plants with potential medicinal usage has increased in recent years because of their effects, including antioxidant, antiviral, antibacterial, and anticancer activity. According to the World Health Organization, nearly 50,000 people die each day throughout the world from infectious diseases (1). Recently, the effectiveness of many synthetic antibiotics has become a serious problem because of the emergence of resistance (2).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and cytotoxic activity of Alnus glutinosa (L.) Gaertn., A. incana (L.) Moench, and A. viridis (Chaix) DC. extracts

Dahija

Haverić²,

Čakar³

et al. 2016

JHSCI

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Introduction: The objective of the present study was to evaluate the antimicrobial and cytotoxic activities of water extracts of leaves and barks from Alnus glutinosa (L.) Gaertn., A. incana (L.) Moench, and A. viridis (Chaix) DC.Methods: The antimicrobial activities of extracts were tested against gram-negative and gram-positive bacteria as well as yeast strains by the agar diffusion method. The cell viability was determined by the Trypan blue dye exclusion method.Results: The largest diameters of inhibition zone (DIZ) were recorded with Staphylococcus aureus ATCC 6538 and Bacillus subtilis 168M. The highest percentage of cell viability was observed with water bark extracts of A. glutinosa (97.46%).Conclusions: Potential antimicrobial properties of A. glutinosa, A. incana, and A. viridis demonstrated in this study, as well as their low levels of toxicity, make them an interesting subject for further studies.

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In vitro interaction of certain antimicrobial agents in combination with plant extracts against some pathogenic bacterial strains

Cited by 50 publications

References 15 publications

Synergistic Effect of Combined Antibiotic and Methanol Extract of Eucalyptus camaldulensis leaf Against Staphylococcus aureus and Pseudomonas aeruginosa

Synergistic Effect of Combined Antibiotic and Methanol Extract of Eucalyptus camaldulensis leaf Against Staphylococcus aureus and Pseudomonas aeruginosa

Efficacy of Anti-Microbial Agents with Ascorbic Acid in Catheter Associated Urinary Tract Infection

Antimicrobial and cytotoxic activity of Alnus glutinosa (L.) Gaertn., A. incana (L.) Moench, and A. viridis (Chaix) DC. extracts

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