In vitro isolation and seroprevalence of Toxoplasma gondii in stray cats and pigeons in Lisbon, Portugal

Waap, Helga; Cardoso, Rita; Leitão, Alexandre; Nunes, Telmo; Vilares, Anabela; Gargaté, Maria João; Meireles, José; Cortes, Hélder; Ângelo, Helena

doi:10.1016/j.vetpar.2012.01.022

Cited by 25 publications

(13 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In Lisboa and the surrounding area, toxoplasmosis seroprevalence was 71% for a sample of 145 cats. Another study (Waap et al, 2012) determined that there was an overall prevalence of 44.2% of T. gondii in cats, which was in line with the 30-40% estimated seroprevalence of T. gondii in cats worldwide (Elmore et al, 2010).…”

Section: Parasitessupporting

confidence: 54%

Routine screening of harmful microorganisms in beach sands: Implications to public health

Sabino

Rodrigues

Costa

et al. 2014

Science of The Total Environment

View full text Add to dashboard Cite

Beaches worldwide provide recreational opportunities to hundreds of millions of people and serve as important components of coastal economies. Beach water is often monitored for microbiological quality to detect the presence of indicators of human sewage contamination so as to prevent public health outbreaks associated with water contact. However, growing evidence suggests that beach sand can harbor microbes harmful to human health, often in concentrations greater than the beach water. Currently, there are no standards for monitoring, sampling, analyzing, or managing beach sand quality. In addition to indicator microbes, growing evidence has identified pathogenic bacteria, viruses, and fungi in a variety of beach sands worldwide. The public health threat associated with these populations through direct and indirect contact is unknown because so little research has been conducted relating to health outcomes associated with sand quality. In this manuscript, we present the consensus findings of a workshop of experts convened in Lisbon, Portugal to discuss the current state of knowledge on beach sand microbiological quality and to develop suggestions for standardizing the evaluation of sand at coastal beaches. The expert group at the "Microareias 2012" workshop recommends that 1) beach sand should be screened for a variety of pathogens harmful to human health, and sand monitoring should then be initiated alongside regular water monitoring; 2) sampling and analysis protocols should be standardized to allow proper comparisons among beach locations; and 3) further studies are needed to estimate human health risk with exposure to contaminated beach sand. Much of the manuscript is focused on research specific to Portugal, but similar results have been found elsewhere, and the findings have worldwide implications.

show abstract

Section: Parasitessupporting

confidence: 54%

Routine screening of harmful microorganisms in beach sands: Implications to public health

Sabino

Rodrigues

Costa

et al. 2014

Science of The Total Environment

View full text Add to dashboard Cite

show abstract

“…Pigeons were used as sentinels for T. gondii oocyst contamination in the city of Lisbon, because pigeons feed from the ground and they are herbivores (Waap et al 2008(Waap et al , 2012. Seroprevalence among pigeons from 33 sites ranged from 4·6 to 65%, suggesting a considerable contamination of several urban areas of the city with T. gondii oocysts.…”

Section: Contamination Of Environment With T Gondiimentioning

confidence: 99%

Epidemiological review ofToxoplasma gondiiinfection in humans and animals in Portugal

et al. 2014

View full text Add to dashboard Cite

Toxoplasmosis is a worldwide zoonosis. However, data from Portugal are limited and a considerable part of the literature is in Portuguese. Currently, the rate of congenital infection in Portugal is unknown, and almost nothing is known of sequelae of congenital toxoplasmosis. There is no recent general population-based serological survey of Toxoplasma gondii in humans in Portugal. In addition, there is little information on genetic characteristics of T. gondii in animals and humans. In the present paper, we review prevalence, clinical spectrum and epidemiology of T. gondii in humans and animals in Portugal. This knowledge should be useful to biologists, public health workers, physicians and veterinarians.

show abstract

“…Waap et al. () isolated the parasite from samples of brain and muscle tissue of cats in a period from 5 to 32 days, and Miller et al. () isolated the protozoan in vitro from vitreous humour samples during 12 days on average.…”

Section: Discussionmentioning

confidence: 99%

“…The Roux flasks were incubated at 37°C in a humidified atmosphere with 5% CO 2 , following the protocol previously published (Waap et al. ; Dubey et al. ).…”

Section: Methodsmentioning

confidence: 99%

Detection and isolation of Toxoplasma gondii from fresh semen of naturally infected dogs in Southern Brazil

Koch

Weiss

Cruz

et al. 2016

Reprod Domestic Animals

View full text Add to dashboard Cite

The aim of this study was to isolate Toxoplasma gondii and determine the viability of the parasite in fresh semen samples of clinically healthy adult dogs naturally infected. Eleven seropositive dogs with T. gondii IgG antibodies from southern Brazil were selected to confirm the presence and viability of T. gondii in fresh semen samples using in vitro isolation in Vero cell culture, polymerase chain reaction (PCR) and sequencing analysis. The presence of viable T. gondii was confirmed by in vitro isolation and PCR in five semen samples. The ITS1 region of the isolated protozoa (TG S4) was amplified and sequenced. The nucleotide sequence obtained was 99% compatible with the T. gondii DNA sequences stored in the GenBank. It has been shown that T. gondii tachyzoites may be isolated in vitro from fresh semen samples of clinically healthy dogs seropositive for T. gondii.

show abstract

In vitro isolation and seroprevalence of Toxoplasma gondii in stray cats and pigeons in Lisbon, Portugal

Cited by 25 publications

References 24 publications

Routine screening of harmful microorganisms in beach sands: Implications to public health

Routine screening of harmful microorganisms in beach sands: Implications to public health

Epidemiological review ofToxoplasma gondiiinfection in humans and animals in Portugal

Detection and isolation of Toxoplasma gondii from fresh semen of naturally infected dogs in Southern Brazil

Contact Info

Product

Resources

About