In vitro isolation, regeneration and purification of yellow mutant in chrysanthemum (Chrysanthemum morifolium) cv. Lalit through ray floret regeneration

Kumar, Gunjeet; Sindhu, S. S.; Kumar, Surendra; Vanlalruati, Vanlalruati

doi:10.56093/ijas.v87i7.71959

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…Organogenesis can be indirectly generated from the callus or direct over the explant without previous callus formation. Direct shoot organogenesis was reported in Chrysanthemum from leaf, petiole, stem, and ray floret explants (Lim et al, 2012;Naing et al, 2014;Kumar et al, 2017;Kazeroonian et al, 2018;Chowdhury et al, 2021;Prathyusha et al, 2021). Also, there are recent reports of indirect shoot organogenesis from leaf and petal explants (Ghosh et al, 2018;Din et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

MORPHOGENIC RESPONSE FROM LEAF EXPLANT OF Dendranthema grandiflora VAR MICROMARGARA

López-Puc¹,

Góngora²,

Cano-Sosa³

et al. 2023

Trop Subtrop Agroecosyst

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Background: Chrysanthemum is one of the most popular landscape plants worldwide. Introducing desirable traits into this plant by conventional breeding has limitations due to cross-incompatibility. An approach to this problem is the introduction of resistance or tolerance traits via plant genetic transformation. In vitro regeneration of plants is necessary for implementing genetic transformation systems; therefore, regeneration via morphogenesis is required before any genetic transformation attempt. Objective: To induce morphogenesis from the leaf explant of Dendranthema grandiflora var Micromargara. Methodology: Micropropagation from node cuttings was induced from node cuttings of 3 cm in length from 6 months-old plants that were used as explants to produce seedlings as source explants. Morphogenesis experiments were done using leaf segments of 1 cm2 from 8-weeks-old in vitro seedlings. The explants were transferred to basal medium Murashige and Skoog at 4.4 gL-1 supplemented with different plant growth regulators. During the morphogenic process, leaves samples were collected to detect which morphogenic process was induced. Results: Node cuttings culture on Murashige and Skoog medium at 3.3 gL-1 supplemented with benzylaminopurine at 2.2 µM yielded a vegetative growth increase from 1 to 7 shoots, 2 to 12 leaves per node cutting, and the stem length of 3 to 9.5 cm. The leaf explant induced three ways of in vitro morphogenesis: direct and indirect roots organogenesis (rhizogenesis) and, shoot organogenesis. Direct rhizogenesis was induced in plant growth regulator-free Murashige and Skoog medium (18 roots/explant). Indirect rhizogenesis from leaf explant was less efficient than direct rhizogenesis, get obtaining six roots per leaf explant cultured on Murashige and Skoog medium at 4.4 g L-1 supplemented with 0.4 µM thidiazuron and 4.5 µM 2,4-dichlorophenoxyacetic acid, and three roots per leaf explant cultured on Murashige and Skoog medium supplemented with 0.4 µM thidiazuron and 9.05 µM 2,4-dichlorophenoxyacetic acid. Indirect shoot organogenesis was induced from leaf explants cultured on Murashige and Skoog at 4.4 g L-1 supplemented with 13.32 µM benzylaminopurine and 4.83 µM naphthalene acetic acid; 50% of explants with callus formed shoots (2 shoots/leaf explant). Through histological analysis it was possible to verify that the morphogenic response obtained was organogenesis. Implications: Regeneration of Dendranthema grandiflora var. Micromargara established herein will allow improvement of the variety through techniques of genetic transformation mediated by Agrobacterium and any other strategy to incorporate or silence genes of interest in the plant. Conclusion: The high capacity to induce roots of Dendranthema grandiflora var Micromargara, may provide an efficient model for further investigating the mechanism of rhizogenesis from leaf explants cultured without plant growth regulators.

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Section: Introductionmentioning

confidence: 99%

MORPHOGENIC RESPONSE FROM LEAF EXPLANT OF Dendranthema grandiflora VAR MICROMARGARA

López-Puc¹,

Góngora²,

Cano-Sosa³

et al. 2023

Trop Subtrop Agroecosyst

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In vitro isolation of a gamma ray induced mutant in chrysanthemum (Dendranthema grandiflora)

et al. 2020

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In the present investigation, an effort was made to develop an efficient ray floret regeneration protocol in order to isolate, purify and establish a novel gamma ray induced mutant in the form of brick red flowers in chrysanthemum (Dendranthema grandiflora Tzvelev.) cv. Tata Century (pink). Maximum survival (60.00%) and callus formation (56.60%) in minimum duration (10.20 days) were recorded when the ray florets were pre-treated with carbendazim (0.2%) + ridomil (0.2%) + 8-HQC (200 mg/l) for 2 h followed by surface sterilization with HgCl2 (0.1%) for a duration of 4 min and cultured on MS medium supplemented with BAP (4.0 mg/l) and NAA (1.0 mg/l). The maximum regeneration of microshoots (66.00%) from the ray floret induced callus was recorded on MS medium fortified with BAP (4.0 mg/l) + NAA (1 mg/l). MS medium supplemented with BAP (4.0 mg/l) + NAA (0.05 mg/l) + GA3 (0.1 mg/l) was found to be best for highest micro-shoot proliferation (80.00%). Highest rooting (87.60%) was induced after inoculating the microshoots individually on half-strength MS medium fortified with 0.5 mg/l NAA and 60 g/l sucrose. Successful acclimatization of in vitro raised plantlets was done in glass jar with polypropylene cap each filled with a mixture of sterilized cocopeat, soilrite and perlite (1:1:1 v/v) supplemented with half-strength MS inorganic salts. After 3-4 weeks of acclimatization the plantlets were successfully transferred to field conditions.

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In vitro isolation, regeneration and purification of yellow mutant in chrysanthemum (Chrysanthemum morifolium) cv. Lalit through ray floret regeneration

Cited by 2 publications

References 12 publications

MORPHOGENIC RESPONSE FROM LEAF EXPLANT OF Dendranthema grandiflora VAR MICROMARGARA

MORPHOGENIC RESPONSE FROM LEAF EXPLANT OF Dendranthema grandiflora VAR MICROMARGARA

In vitro isolation of a gamma ray induced mutant in chrysanthemum (Dendranthema grandiflora)

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