In vitro litholytic activity of extracts and phenolic fractions of some medicinal plants on urinary stones

Abstract

<jats:p>Objective: This study was carried out to evaluate the efficiency of plant extracts used in traditional medicine on the dissolution of oxalo-calcic and cystine stones. Also, the efficiency of phenolic fractions (Tannins and Flavonoids) for the plants that gave us the best stone dissolution rate. Subjects and methods: Kidney stone of oxalo-calcic and cystine stones were incubated in vitro for 8 weeks in the presence of five plants extracts (hydro-ethanolic and aqueous extracts). NaCl solution (0,9 %) used as negative control and sodium citrate solution at 3 mM/L as a positive control. The studied plants were Herniaria hirsuta L. aerial parts, Opuntia ficus-indica L. flowers, Zea mays L. stigmata, Ammi visnaga L. seeds and Ziziphus lotus L. fruits. After 2, 4, 6 and 8 weeks the stones were weighed after 18 h drying at 40°C and the dissolution rate of the stones and the pH of the solution were measured.Results: After eight weeks, all the plant extracts and phenolic fractions had revealed a significant effect to dissolve oxalo-calcic and cystine stones in comparison to the control solutions. The best result of cystine stones was showed with aqueous extract of H. hirsuta aerial parts which had a dissolution rate of 88,91 %. Concerning the dissolution rate of oxalo-calcic stones the aqueous extract of Z. mays had the best results with 68 % against 19 % for the sodium citrate solution. The dissolution rate of the fractions studied showed a better result for Z. mays flavonoids fraction toward oxalo-calcic stones, and Z. mays tannins fraction for cystine stones. The pH undergoes a non-negligible linear increase over the eight weeks for all extracts and phenolic fractions and both kidney stones.Conclusion: The tested plants' extracts and the phenolic fractions for the plants that gave us the best stone dissolution rate were able to dissolve oxalo-calcic and cystine stones. To confirm the efficiency of these plant extracts on the treatment of oxalo-calcic and cystine stones multiple in vivo tests could be made.</jats:p>

Medicinal Plants Used by Traditional Healers in Algeria: A Multiregional Ethnobotanical Study

“…These findings are in agreement with those previously reported in different neighboring regions (Benarba et al, 2015;Bouasla and Bouasla, 2017;Chaachouay et al, 2020). Besides these species, Parietaria officinalis L. was found to possess an FL of 100% for kidney diseases which is consistent with findings previously reported in North-West Algeria (Benarba, 2016) and Morocco (Ammor et al, 2020). Inconsistent with our previous findings in both North-West (Benarba et al, 2015) and South-West Algeria (Benarba, 2016), T. vulgaris was the only species having the highest FL of 100% for skin diseases.…”

Inventory of Medicinal Plants Used Traditionally to Manage Kidney Diseases in North-Eastern Morocco: Ethnobotanical Fieldwork and Pharmacological Evidence

“…According to the traditional knowledge of the North-Eastern Moroccan population, this plant is considered a powerful and common medicinal herb that has shown significant results in treating kidney stones; renal colic; pyelonephritis; kidney pain; diuretic; detoxification of the kidneys; and polycystic kidney disease. From a pharmacological point of view, the aqueous extract of the aerial part of this plant has an inhibitory effect on the crystallization of calcium oxalate in vitro at doses of 0.0625 mg/mL and 0.5% of plant extracts in physiological solution (9 g of NaCl /L) [ 96 , 97 ], and in vivo at a concentration of 50 mg/mL [ 98 ], also has an effect on cystine stones in different patients with congenital cystinuria at a dose of 20 g/L [ 101 ]. Phytochemical studies have reported and identified some components of H. hirsuta include flavonoids, coumarin, tannins and saponins [ 100 , 171 , 172 , 173 ].…”

Role Globularia in the inhibition of calcium oxalate crystallization in vitro study

“…O. ficus-indica extract can reach a DR of 64 % and 45 % of the total mass of cystine stones and calcium oxalate stones, respectively. The DR of H. hirsute extracts gave 61 % for cystine stones, whereas it is 50 % when using calcium oxalate stones [16].…”