In vitro maturation and fertilization of goat oocytes

Smedt, Véronique De; Crozet, Nicole; Ahmed-Ali, Michèle; Martino, A.; Cognie, Y.

doi:10.1016/0093-691x(92)90103-x

Cited by 66 publications

(30 citation statements)

References 15 publications

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“…The results obtained (Table 1) were better for oocytes with cumulus cells at the time of fertilization, and exceeded those achieved by Younis et al (1992). In contrast to the report of DeSmedt et al (1992) the cumulus cells were confirmed to play an important role in fertilization and continued viability as demonstrated earlier for bovine IVF (Brackett et al, 1989).…”

Section: Discussioncontrasting

confidence: 49%

“…Immature mammalian embryos can mature in 23-27 h in vitro (Watson et al, 1992). A maturation interval of 27 h was found to be superior for caprine oocytes (DeSmedt et al, 1992;Keskintepe et al, 1993;Martin0 et al, 1994). The best insemination intervals for mammalian IVMFC were indicated as 24 h for bovine (Younis et al, 19911, 18 h for sheep (Crozet et al, 1987), and 18-24 h for goat (DeSmedt et al, 1992, Keskintepe et al, 1993.…”

Section: Discussionmentioning

confidence: 99%

“…no. H7005) + 2 mM caffeine (Sigma)ml-' for 15 min, or 0.1 PM calcium ionophore A23 187 (Yang et al, 1993) for 1 min, or by exposure to 7.35 mM calcium lactate during the insemination interval (DeSmedt et al, 1992). The calcium ionophore was prepared by combining 50 ~1 of A23187 with 20 ~1 dimethylsulfoxide (DMSO).…”

Section: Sperm Preparation and In Vitro Fertilization Fivf)mentioning

confidence: 99%

“…Sperm cells were treated similarly as described by DeSmedt et al (1992) using Defined Medium (DM), (Brackett and Oliphant, 1975) prepared without BSA but with 20% FBS and 25 mM HEPES, modified DM (mDM), final pH 7.3. Briefly, sperm were washed in mDM by centrifugation at 320 g for 10 min; after discarding supematant washings pelleted sperm cells were overlaid with 1.5 ml of mDM and allowed to swim up for 45 min.…”

Section: Sperm Preparation and In Vitro Fertilization Fivf)mentioning

confidence: 99%

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Procedural improvements for in vitro production of viable uterine stage caprine embryos

Keskintepe

Luvoni

Rzucidlo

et al. 1996

Small Ruminant Research

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Efforts to improve proportions of caprine immature oocytes developing into viable uterine-stage embryos in vitro involved study of 1924 oocytes in experiments designed to examine influences of fertilization media, sperm incubation temperatures, sperm treatment procedures, different protein supplementations, and different insemination intervals. Oocytecumulus complexes (OCCs) were matured during 27 h in TCM-199 supplemented with 20% FBS, 100 pug LH ml-', 0.5 pg FSH ml-', and 1 pg Estradiol-17-P ml-' at 38.5"C in a humidified 5% CO,, 5% O,, and 90% N, atmosphere. Freshly collected sperm were washed and incubated at either 22°C or 38.5"C for 5 h and then treated with either 0.1 PM calcium ionophore A23187 for 1 min, or with 7.35 mM calcium lactate in the presence of oocytes during the insemination interval, or with 100 pg heparin +2 mM caffeineml -' for 15 min. 'The interval for insemination was experimentally varied, i.e. 14 or 24 h. Results showed that: (a) when used as a fertilization medium mDM supported more blastocyst development than TALP (10.5% vs. O%, P < 0.05); (b) incubation temperatures of 22°C or 38.5"C prepared goat spermatozoa equally for capacitation in mDM containing 20% FBS; (c> when oocytes were inseminated with sperm incubated in mDM with 20% FBS and capacitated with calcium lactate more embryos reached the blastocyst stage (P < 0.05) than after incubation in the same conditions but after sperm capacitation with heparin, and A23187 (31.8% vs. 24.2% and 10.2%, respectively; (d) a 24 h insemination interval was not superior to 14 h when sperm were incubated with either 20% FBS or 6 mg BSAml-' and capacitated with calcium lactate (P > 0.05). Three morulae resulting from the best conditions in this work (FBS, calcium lactate, 14 h insemination) were transferred into the uterine horn ipsilateral to the corpus luteum of a recipient and two normal female kids were born after normal gestation. This is the first report in which it has been possible to consistently take caprine development to the blastocyst stage in vitro, and to obtain offspring following uterine transfer. Methodology reported here should facilitate implementation of new reproductive and genetic strategies in goat breeding.

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Section: Discussioncontrasting

confidence: 49%

Section: Discussionmentioning

confidence: 99%

Section: Sperm Preparation and In Vitro Fertilization Fivf)mentioning

confidence: 99%

Section: Sperm Preparation and In Vitro Fertilization Fivf)mentioning

confidence: 99%

See 2 more Smart Citations

Procedural improvements for in vitro production of viable uterine stage caprine embryos

Keskintepe

Luvoni

Rzucidlo

et al. 1996

Small Ruminant Research

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“…Polyspermy is a common problem of in vitro fertilization (IVF) in the cow (Wang et al 1997, Roh et al 2002, Coy et al 2005, sheep (Fukui et al 1988, Slavik et al 2005, goat (De Smedt et al 1992, Mogas et al 1997, human (van der Ven et al 1985, Balakier 1993, Aoki et al 2005, and in particular, pig , Hao et al 2006. Since the reported levels of polyspermy after IVF are higher than 50% of pig oocytes and as high as 25% of cow oocytes, these two species were chosen as models for this study.…”

Section: Introductionmentioning

confidence: 99%

Hardening of the zona pellucida of unfertilized eggs can reduce polyspermic fertilization in the pig and cow

Coy¹,

Grullón²,

Cánovas³

et al. 2007

Reproduction

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One of the proposed mechanisms of polyspermy block is an increased resistance of the zona pellucida (ZP) to proteolytic digestion (ZP hardening) as a consequence of cortical granule exocytosis that occurs soon after fertilization. However, evidence is available that the zonae pellucidae of freshly ovulated pig and cow oocytes harden considerably before fertilization. It was thought that such pre-fertilization ZP hardening could be involved in the control of polyspermy, and its lack in the oocytes matured in vitro could be one of the reasons for the extremely high incidence of polyspermy in pig in vitro fertilization (IVF). To test this hypothesis, two different types of cross-linking reagents were employed and their effects on ZP hardening and IVF efficiency were examined. The sulfhydryl-reactive cross-linkers produced a slight hardening of ZP (P!0.001) of treated oocytes compared with control oocytes, and totally inhibited sperm penetration into pig oocytes after IVF. In the cow, sperm penetration into eggs was reduced to 10%. It is proposed that formation of disulfide bonds in ZPor blocking of SH groups in the oocyte plasma membrane proteins prevents sperm penetration. An amine-reactive cross-linker was then assayed and produced strong ZP hardening, increasing the incidence of monospermy in both pig and cow oocytes after fertilization. When the cross-linker concentration was optimized, a 45% improvement for pig IVF efficiency was reached. It is proposed that the observed physiological ZP hardening is a mechanism to control polyspermy, differentially affecting various mammalian species and can be imitated by the use of amine-reactive cross-linkers during IVF.

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Mitogen-activated protein kinase activity during goat oocyte maturation and the acquisition of meiotic competence

Dedieu¹,

Gall²,

Crozet³

et al. 1996

Mol. Reprod. Dev.

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In vitro maturation and fertilization of goat oocytes

Cited by 66 publications

References 15 publications

Procedural improvements for in vitro production of viable uterine stage caprine embryos

Procedural improvements for in vitro production of viable uterine stage caprine embryos

Hardening of the zona pellucida of unfertilized eggs can reduce polyspermic fertilization in the pig and cow

Mitogen-activated protein kinase activity during goat oocyte maturation and the acquisition of meiotic competence

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