In vitro maturation of bovine cumulus–oocyte complexes in undiluted follicular fluid: effect on nuclear maturation, pronucleus formation and embryo development

Avery, B.; Strøbech, Lotte; Jacobsen, Tina; Bøgh, Ingrid Brück; Greve, T.

doi:10.1016/s0093-691x(02)01139-1

Cited by 37 publications

(39 citation statements)

References 63 publications

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“…Similarly, previous studies have reported that oocyte maturation in FF or addition of a certain concentration of FF can even improve embryonic development (Ali et al 2004, Coleman et al 2007. By contrast, another study showed that maturation of oocytes in FF reduces developmental competence (Avery et al 2003). Differences between studies might be due to the use of FFaspirated at different stages of follicular growth or the estrous cycle.…”

Section: Discussionmentioning

confidence: 90%

Effects of Escherichia coli- and Staphylococcus aureus-induced mastitis in lactating cows on oocyte developmental competence

Asaf

Leitner²,

Furman³

et al. 2014

Reproduction

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Mastitis is associated with decreased fertility in dairy cows. In the current study, we created an experimental model to simulate shortterm mastitis by a single intramammary administration of Gram-negative endotoxin of Escherichia coli origin (GK), or Gram-positive toxin of Staphylococcus aureus origin (GC), to examine the effect of mastitis on oocyte developmental competence. Healthy Holstein cows were synchronized, and follicular fluid (FF) of cows treated with GC or GK and of uninfected cows (controls) was aspirated from the preovulatory follicles by transvaginal ultrasound procedure. The aspirated FF was used as maturation medium for in vitro embryo production. The distribution of matured oocytes into different cortical granule classes and meiotic stages was affected by GK administration (P!0.05) but not by GC administration. The proportion of oocytes that cleaved to two-and four-cell stage embryos (44 h postfertilization) was lower in both GC and GK groups than in controls (P!0.05). Blastocyst formation rate (7-8 days postfertilization) was lower in the GK group (P!0.05) and numerically lower in the GC group compared with their uninfected counterparts. The total cell number in blastocysts did not differ among groups; however, the apoptotic index was higher in the GC group (P!0.05), but not in the GK group, relative to controls. Examining mRNA relative abundance in oocytes and early embryos revealed mastitis-induced alterations in PTGS2 (COX2), POU5F1, and HSF1 but not in SLC2A1 (GLUT1) or GDF9. Results indicate a differential disruptive effect of mastitis induced by GK and GC on oocyte developmental competence in association with alterations in maternal gene expression.

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Section: Discussionmentioning

confidence: 90%

Effects of Escherichia coli- and Staphylococcus aureus-induced mastitis in lactating cows on oocyte developmental competence

Asaf

Leitner²,

Furman³

et al. 2014

Reproduction

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“…Although preimplantation embryos may develop during IVC period, obvious differences exist between their developmental rates compared to those developed in vivo. For instance, approximately 60-80% of in vivo matured bovine oocytes are competent to reach to the metaphase II stage while only 25-40% of in vitro matured oocytes reach to this stage (Blondin et al, 2002;Dieleman et al, 2002;Avery et al, 2003). Failure to fertilize and develop to the blastocyst stage may show the lack of vital factors in the culture media which normally are available in vivo.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect of Insulin on <i>in vitro</i> Development of Immature Bovine Oocytes

Dashtizad¹,

Wahid²,

Yusoff³

et al. 2010

American Journal of Animal and Veterinary Sciences

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Problem statement: Development of efficient culture system to support embryonic development would be valuable when quality of produced embryos was important. However, the rate of bovine embryo production in vitro was still lower than expected. Present study, including of three experiments, was carried out to investigate the effect of insulin on nuclear maturation and subsequent development of immature bovine oocytes and in vitro fertilized embryos. Approach: Grade one cumulus-oocyte-complexes harvested from slaughterhouse ovaries were selected and randomly allocated in each treatment groups. In experiment 1, in vitro maturation medium (Hepes-buffered medium 199 + fetal calf serum + gonadotrophins + antibiotics) supplemented with 0 (control), 1, 10, 20 and 100 µg mL −1 of insulin. In experiment 2, to eliminate the effect of serum and hormones, Hepesbuffered medium 199 was supplemented with 1 mg mL −1 polyvinyl alcohols (PVA) and same levels of insulin. In experiment 3, the effect of insulin on bovine in vitro embryo development was assessed. Presumptive zygotes were randomly cultured in synthetic oviductal fluid added with 0 (control), 1, 10, 20 and 100 µg mL −1 of insulin. Results: In experiment 1, nuclear maturation and embryo development rates were significantly higher in 1 and 10 µg mL −1 compared with other groups (P<0.05). In experiment 2, both maturation and cleavage rate significantly increased in 1 and 10 µg mL −1 insulin.The only treatment resulted in higher hatchability was 10 µg mL −1 insulin (17.1±2.34%) compared with control (11.34±3.94). In experiment 3, cleavage and morula rates were significantly greater in 1 and 10 µg mL −1 insulin compared with other groups; although the highest rates resulted by using 10 µg mL −1 . Conclusion: Obtained results show that inclusion of 10 µg mL −1 insulin in maturation and culture medium exerted beneficial effects on nuclear maturation of bovine oocytes and in vitro embryo development till morula stage.

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“…The FF has been successfully incorporated in IVM media for bovine [18,19], human [20,21], porcine [22] and equine [23] oocytes. Especially, in buffalos, there are reports that the maturation rate was increased by adding 40% [24] or 100% [25] of high concentration FF to IVM media.…”

Section: Discussionmentioning

confidence: 99%

“…Especially, in buffalos, there are reports that the maturation rate was increased by adding 40% [24] or 100% [25] of high concentration FF to IVM media. Also, human [18] immature oocytes were cultured in vitro with Ham's F-10 supplemented with human FF (hFF) or human fetal cord serum (hFCS). The maturation rate in the hFF group was 55.8%, significantly higher than the 35.9% in the hFCS group.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the hFF group had a significantly higher fertilization rate than the hFCS group (81.0% and 31.6%, respectively). Following that report [18], the IVM system for minke whale oocytes was modified from Medium 199 containing 20% whale fetal cord serum (wFCS) to Medium 199 containing 50% wFF. The modifications resulted in a proportion of matured oocytes which was similar to a previous IVM study (approximately 30%) after IVM culture for only 28-30 h instead of 96-120 h [1][2][3].…”

Section: Discussionmentioning

confidence: 99%

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Attempt at In Vitro Maturation of Minke Whale (Balaenoptera Bonaerensis) Oocytes Using a Portable CO2 Incubator

Iwayama

Ishikawa²,

Ohsumi³

et al. 2005

Journal of Reproduction and Development

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Abstract. The present study was conducted to investigate whether a portable CO2 incubator was effective for in vitro maturation (IVM) of bovine, porcine and minke whale oocytes, and the effect of maturation media supplemented with different hormones; porcine follicle stimulating hormone (pFSH), estradiol-17β (E2), or pregnant mare's serum gonadotropin (PMSG): human chorionic gonadotropin (hCG) for minke whale immature oocytes was also examined. In vitro maturation rates of bovine and porcine oocytes cultured in the portable CO2 incubator were not significantly different from the standard CO2 incubator. In minke whale IVM culture using the portable incubator, the maximum expansion of cumulus mass was observed by pFSH/E2 and PMSG/hCG at the end of IVM culture. Moreover, the IVM culture period was shortened to 28-30 h from 96-120 h previously reported. The proportion of matured oocytes cultured in the medium supplemented with pFSH/E2 (26.7%) was significantly higher (P<0.05) than that with PMSG/hCG (6.9%). The present study indicates that a portable CO2 incubator is a useful device for minke whale IVM culture on a research base ship, and the addition of pFSH/E2 into an IVM medium enhanced cumulus expansion and the proportion of minke whale matured oocytes.

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In vitro maturation of bovine cumulus–oocyte complexes in undiluted follicular fluid: effect on nuclear maturation, pronucleus formation and embryo development

Cited by 37 publications

References 63 publications

Effects of Escherichia coli- and Staphylococcus aureus-induced mastitis in lactating cows on oocyte developmental competence

Effects of Escherichia coli- and Staphylococcus aureus-induced mastitis in lactating cows on oocyte developmental competence

Synergistic Effect of Insulin on <i>in vitro</i> Development of Immature Bovine Oocytes

Attempt at In Vitro Maturation of Minke Whale (Balaenoptera Bonaerensis) Oocytes Using a Portable CO2 Incubator

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