In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India

Bisht, Surendra Singh; Bisht, N. S.; Bhandari, Snehlata

doi:10.1007/s12298-011-0091-5

Cited by 24 publications

(16 citation statements)

References 11 publications

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“…Upon lowering or higher concentrations of BAP, a reduction in the number of shoots per culture was recorded ( Table 1). The effect of BAP on multiple shoot bud differentiation has been demonstrated in number of cases using nodal explant (Patil 1998;Faisal et al 2007;Chavan et al 2011a, Bisht et al 2012. In the present case, BAP alone proved to be more effective than other phytohormones and their combinations.…”

Section: Resultssupporting

confidence: 49%

An efficient in vitro regeneration of Ceropegia noorjahaniae: an endemic and critically endangered medicinal herb of the Western Ghats

Chavan

Nalawade

Gaikwad

et al. 2014

Physiol Mol Biol Plants

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An efficient protocol was developed for the rapid in vitro multiplication of an endemic and critically endangered medicinal herb, Ceropegia noorjahaniae Ans., via enhanced axillary bud proliferation from nodal explants. The effects of phytohormones [6-benzylaminopurine (BAP), kinetin (Kin) thidiazuron (TDZ), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or α-naphthalene acetic acid (NAA)] on in vitro regeneration were investigated. The highest number of shoots (18.3 ± 1.3), maximum shoot length (10.1 ± 0.8 cm) and the highest response of shoot induction (95 %) were recorded on MS medium supplemented with 2.0 mg/l BAP. Rooting was best achieved on half-strength MS medium augmented with IBA (1.0 mg/l). Half-strength MS medium supplemented with BAP (4 mg/l) and sucrose (5 %, w/v) produced an average of 5.6 flower buds per microshoots with highest (90 %) flower bud induction response. The plantlets regenerated in vitro with well-developed shoot and roots were successfully established in pots containing sterile sand and coco peat (1:1) and grown in a greenhouse with 85 % survival rate. The regenerated plants did not show any detectable morphological variation. The developed method can be successfully employed for large-scale multiplication and conservation of C. noorjahaniae.

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Section: Resultssupporting

confidence: 49%

An efficient in vitro regeneration of Ceropegia noorjahaniae: an endemic and critically endangered medicinal herb of the Western Ghats

Chavan

Nalawade

Gaikwad

et al. 2014

Physiol Mol Biol Plants

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“…In vitro propagation has been successfully applied for the conservation of a large number of endangered plant species that have limited reproductive capacity and exist in threatened habitats (Fay 1992;George et al 2008;Pence 2010). Recently, there have been a number of established protocols for in vitro propagation of rare and threatened medicinal plants such as Dysosma versipellis (Jiang et al 2012), Polygonatum verticillatum (Bisht et al 2012), Pseudarthria viscida (Cheruvathur and Thomas 2011), and Thymus lotocephalus (Coelho et al 2012).…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered medicinal plant

Prathanturarug

Pheakkoet

Jenjittikul

et al. 2012

Physiol Mol Biol Plants

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In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered species of Stemonaceae, was established. Nodal and internodal explants were cultured on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ) alone or in combination with 1-naphthaleneacetic acid (NAA). When cultured on MS medium supplemented with 18.16 μM TDZ with 0.54 μM NAA for 8 weeks and then on MS medium without plant growth regulator (PGR) for 8 weeks, nodal explants demonstrated a responding frequency of 91.67 % and a shoot regeneration rate of 5.46 shoots/responding explant. Furthermore, internodal explants demonstrated a responding frequency of 17.17 % with 11.17 shoots/responding explant. The regenerated shoots were rooted using a two-step protocol by culturing for 4 weeks on MS medium supplemented with various concentrations of NAA and Indole-3-butyric acid (IBA) and 8 weeks on MS medium without PGR. When 5.40 μM NAA was used as a PGR, the maximum root induction rate was 69.45 %. Regenerated plantlets were successfully transferred to soil.

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“…It has also been studied for antimalarial and antioxidant, metal accumulating, insecticidal, antibacterial, antipyretic, tracheorelaxant and antiinflammatory, antispasmodic and antidiarrheal, antinociceptive, aphrodisiac [199,235,[238][239][240][241][242]. Lipoxygenase, urease inhibition, anti tyrosinase activity of the aerial parts of the P. verticillatum was also observed which were attributed to the presence of saponins, alkaloids, flavonoids, phenols, tannins and terpenoids in considerable amount [243][244][245].…”

Section: Polygonatum Verticillatummentioning

confidence: 99%

Phytochemical, Botanical and Biological Paradigm of Astavarga Plants- The Ayurvedic Rejuvenators

Misra¹

2018

JONAM

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Astavarga is nature's most extravagant group of flowering plants found in Himalayan region. As the name Astavarga indicates, it contains eight medicinal plants belonging to Zingiberaceae, Orchidaceae and Liliaceae family viz, Kakoli, Kshirakakoli, Meda, Mahameda, Jeevak, Risbhaka, Riddhi and Vridddhi. As per Ayurveda this group of medicinal plants is classified as Rasayana (Rejuvenation) and is important constituents of preparations like Chyawanprasha rasayan, Ashtavarga churna, Brimhanigutika and Vajikaraghrita, etc. Astavarga plants are mostly used to treat sexual disorders, physical weakness, body pain, strengthen the immune system and as an overall tonic. The plants under this group have been the subject of limited biochemical phytochemical and biological activities investigations. Taking into consideration the therapeutic significance of Astavarga plants as described in the ancient Ayurvedic system of medicine, the detailed phytochemical and pharmacological studies appear imperative to scientifically validate the ancient claims. Thus, the present review article provides detailed information on the Ayurvedic uses, habit and habitats, botanical descriptions, chemical constituents and biological activities reported for this important group of plants. The chemical structures of the isolated compounds from these plants and their reported biological activities in the literature have also been included.The information provided in the present review may stimulate the researchers to explore their phytochemical and pharmacological properties which have not yet been attempted, comprehensively. It will generate the interest on "reverse pharmacology" approach to validate the knowledge that has been known from ancient times.

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In vitro micropropagation in Polygonatum verticillatum (L.) All. an important threatened medicinal herb of Northern India

Cited by 24 publications

References 11 publications

An efficient in vitro regeneration of Ceropegia noorjahaniae: an endemic and critically endangered medicinal herb of the Western Ghats

An efficient in vitro regeneration of Ceropegia noorjahaniae: an endemic and critically endangered medicinal herb of the Western Ghats

In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered medicinal plant

Phytochemical, Botanical and Biological Paradigm of Astavarga Plants- The Ayurvedic Rejuvenators

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