In Vitro Migratory Response of Rat Peritoneal Macrophages and Mast Cells Towards Chemotactic Factors and Growth Factors

Czarnetzki, Beate M.; Wüllenweber, Imke

doi:10.1111/1523-1747.ep12470299

Cited by 10 publications

(3 citation statements)

References 18 publications

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“…Specifically, evidence has been presented that mast cells can express MHC class II molecules [1][2][3][4] and that they are active antigen presenting cells [3,5,6]. Furthermore, the CD34 þ mast cell precursors, as well as immature and mature mast cells, are capable of phagocytosis and chemotaxis [7][8][9][10][11][12]. All these activities imply that mast cells are able to interact with endothelial and with circulating and resident tissue effector cells and that they express adhesion molecules during these processes.…”

Section: Introductionmentioning

confidence: 99%

Human Leukaemic (HMC‐1) and Normal Skin Mast Cells Express β2‐Integrins: Characterization of β2‐Integrins and ICAM‐1 on HMC‐1 Cells

et al. 1997

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Weber S, Babina M, Feller G, Henz BM. Human Leukaemic (HMC-1) and Normal Skin Mast Cells Express b2-Integrins: Characterization of b2-Integrins and ICAM-1on HMC-1 Cells. Scand J Immunol 1997;45:471-481 Mast cells are bone marrow-derived, ubiquitous connective tissue resident cells. However, their mechanisms of migration, the distribution of immature and mature cells and their interaction with other inflammatory cells are largely unclarified. Possibly, b2-integrins play an important role in these processes. In the present investigation, the authors studied the expression and regulation of the b2-integrins LFA-1 (CD11a/CD18), Mac-1 (CD11b/CD18), p150,95 (CD11c/CD18) and of the LFA-1/Mac-1 counter-receptor intercellular adhesion molecule-1 (ICAM-1; CD54) on leukaemic (HMC-1 cell subclone 5C6) and on normal mature human skin mast cells. The HMC-1 cells clearly expressed CD11a, CD18 and CD54, while expression of CD11b and CD11c was low. The apparent molecular weights were 180 kDa (CD11a), 95 kDa (CD18) and 90 kDa (CD54) as determined by Western blot analysis. Phorbol myristate acetate (PMA) induced a time-and dose-dependent up-regulation of CD11a, CD11b, CD11c, CD18 and CD54 that was inhibited by cycloheximide, suggesting a dependence on de novo protein synthesis. Enhanced expression of CD11a, CD11b, CD11c and CD18 could also be confirmed at the gene level as demonstrated by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Increased expression of LFA-1/ICAM-1 in response to PMA was accompanied by strong enhancement of homotypic cell aggregation, suggesting that newly synthesized LFA-1/ICAM-1 is functionally active. In order to determine a physiologically relevant way of mast cell b2-integrin modulation, several cytokines and chemotactic mediators (interleukin-4, IL-4; nerve growth factor b, NGFb; C5a; and leukotriene B4, LTB4) were tested for their influence on adhesion molecule cell surface density. Only LTB4 was shown specifically to up-regulate CD11a and CD18, but not CD11b or CD11c. The presence of CD11a, CD11c and CD18 could be confirmed on a low percentage of normal skin mast cells by immunofluorescence, using a double staining technique. In comparison to normal skin, a significantly higher percentage of CD18 þ mast cells was found in inflammatory dermatoses such as psoriasis vulgaris, atopic dermatitis and lichen planus. Therefore, mast cell b2-integrins possibly play an important role during homing of immature mast cells as well as during the interaction of activated mast cells with other inflammatory cells.

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Section: Introductionmentioning

confidence: 99%

Human Leukaemic (HMC‐1) and Normal Skin Mast Cells Express β2‐Integrins: Characterization of β2‐Integrins and ICAM‐1 on HMC‐1 Cells

et al. 1997

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“…Mast cells are resident cells of the dermis, while basophils infiltrate from the blood into the skin only in response to inflammatory stimuli [1,2,12]. Both mast cells and baso phils contain histamine in their cytoplasmic granules which is stored by covalent binding to glycosaminoglycans (GAG).…”

Section: Introductionmentioning

confidence: 99%

Modulatory Effects of Glycosaminoglycans and Histamine on Lymphocyte Mitogenesis

Czarnetzki¹,

Wüllenweber²

1989

Skin Pharmacol Physiol

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Since mast cells have previously been shown to be potent modulators of lymphocyte function, the effect of varying concentrations of three mast cell- or basophil- as well as skin ground substance-derived glycosaminoglycans (GAG; heparin, chondroitin sulfate, hyaluronic acid) and of histamine was investigated on rat spleen cell ³H-thymidine incorporation in the absence and presence of mitogens. Modulation proved to be different for the three GAG: chondroitin sulfate was inhibitory and hyaluronic acid enhancing, while heparin exhibited a more complex pattern, with inhibition in control and phytohemagglutinin-stimulated cultures and enhancement in concanavalin A-driven cultures, in parallel to histamine. The data suggest that ground substance GAG as well as mast cell- and basophil-derived mediators in the skin can have a marked and complex modulatory effect on the function of lymphocytes.

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“…Some authors have recently reported that mast cells can function as antigen‐presenting cells, 15 while others have shown that these cells have phagocytic properties. 16 …”

mentioning

confidence: 99%

Search for evidence of a Th2 profile in HIV+ patients

et al. 2000

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In Vitro Migratory Response of Rat Peritoneal Macrophages and Mast Cells Towards Chemotactic Factors and Growth Factors

Cited by 10 publications

References 18 publications

Human Leukaemic (HMC‐1) and Normal Skin Mast Cells Express β2‐Integrins: Characterization of β2‐Integrins and ICAM‐1 on HMC‐1 Cells

Human Leukaemic (HMC‐1) and Normal Skin Mast Cells Express β2‐Integrins: Characterization of β2‐Integrins and ICAM‐1 on HMC‐1 Cells

Modulatory Effects of Glycosaminoglycans and Histamine on Lymphocyte Mitogenesis

Search for evidence of a Th2 profile in HIV+ patients

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