In vitro minimum growth for conservation of Drosophyllum lusitanicum

Gonçalves, Sandra; Romano, Anabela

doi:10.1007/s10535-007-0163-0

Cited by 25 publications

(16 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Slow growth is usually achieved by reducing the culture temperature, by modifying culture media with supplements of osmotic agents and growth inhibitors, or by removing growth promoters to reduce the cellular metabolism of the material, striving to maximize the time between subcultures (Gonçalves & Romano, 2007;Lata et al, 2010;.…”

Section: Introductionmentioning

confidence: 99%

In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions

Silva

Scherwinski‐Pereira

2011

Pesq. agropec. bras.

View full text Add to dashboard Cite

-The objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol -at 1, 2 and 3%) and abiscisic acid -ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L -1 ). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20 o C was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20 o C on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of recovered shoots.Index terms: ex situ conservation, germplasm bank, micropropagation. Conservação in vitro dePiper aduncum e Piper hispidinervum sob condições de crescimento lento Resumo -O objetivo deste trabalho foi avaliar a conservação in vitro dePiper aduncum e P. hispidinervum, em condições de crescimento lento. Os brotos foram armazenados a baixas temperaturas (10, 20 e 25ºC), e o meio de cultura foi suplementado com agentes osmóticos (sucrose e manitol -a 1, 2 e 3%) e ácido abcísico -ABA (0, 0,5, 1,0, 2,0 e 3,0 mg L -1 ). Após seis meses de armazenamentos os brotos foram avaliados quanto à sobrevivência e à rebrotação. A baixa temperatura de 20 o C foi efetiva para a conservação in vitro de brotos de P. aduncum e P. hispidinervum. Culturas in vitro, mantidas a 20 o C em meio MS apresentaram 100% de sobrevivência com o crescimento lento dos brotos. A presença de manitol ou ABA, no meio de cultura, afetou negativamente o crescimento de brotos, o que foi evidenciado pelo baixo índice de sobrevivência dos brotos.Termos para indexação: conservação ex situ, banco do germoplasma, micropropagação.

show abstract

Section: Introductionmentioning

confidence: 99%

In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions

Silva

Scherwinski‐Pereira

2011

Pesq. agropec. bras.

View full text Add to dashboard Cite

show abstract

“…En varias especies de Zingiberaceae el periodo máximo de conservación sin subcultivos fue de 12 meses, en medio de cultivo MS a la mitad de su concentración más la adición de bencil adenina y 30 g/l de sacarosa y 20g/l de manitol (Mohanty et al 2013). En Drosophyllum lusitanicum se alcanzó el 100% de sobrevivencia a los 8 meses de cultivo en medio con la adición de 20 g dm -3 de manitol y 60 g dm -3 de sacarosa (Gonçalves & Romano 2007). Con bajas cantidades de manitol también se incrementó la sobrevivencia y viabilidad de las plantas de Dioscorea alata (Borges et al 2004).…”

Section: Enraizamientounclassified

“…Por su parte la los explantos cultivados con la adición de las diferentes concentraciones de sacarosa presentaron valores intermedios de mortalidad; por el contrario según Gonçalves & Romano (2007) en Drosophyllum lusitanicum el incremento en la concentración de sacarosa permitió la sobrevivencia de los cultivos después de 8 meses de almacenamiento. Así también, la adición de 30g/l de sacarosa como única fuente de carbono permitió entre el 91.3-100% de sobrevivencia de explantes de pistacho (Akdemir et al 2013).…”

Section: Enraizamientounclassified

See 1 more Smart Citation

Conservación de plantas regeneradas in vitro y análisis de la variación somaclonal de Cinchona officinalis, Linneo

González¹

View full text Add to dashboard Cite

“…Thus, for this type of in vitro preservation, it is necessary to analyze the survival rate, in vitro and after acclimatization. Gonçalves & Romano (2007) evaluated the survival and morphology of plants of Drosophyllum lusitanicum (L.) Link, a threatened species, and the results presented show that cultures can be conserved in vitro for eight months at 5 °c. Pretreatment of plants at low temperature (15 ± 2 °c) for 10 days before transfer to greenhouse conditions increased the survival percentage (malik et al 2009).…”

Section: Introductionmentioning

confidence: 99%

In vitro culture at low temperature and ex vitro acclimatization of Vriesea inflata an ornamental bromeliad

Pedroso¹,

Lazarini²,

Tamaki³

et al. 2010

Rev. bras. Bot.

View full text Add to dashboard Cite

− (In vitro culture at low temperature and ex vitro acclimatization of Vriesea inflata an ornamental bromeliad). In vitro culture by seeds is a technique for preservation of threatened species because it may provide a large number of plants with genetic diversity. The bromeliad Vriesea inflata (Wawra) Wawra, an ornamental bromeliad, is extensively and illegally collected from the nature and must be preserved. it is possible to form plant threatened collections in vitro by reducing the temperature of culture, while occupying little space, with the consequent reduction of maintenance costs. This work evaluated the influence of temperature on in vitro growth and morphology of plants of V. inflata, with the aim of establishing a slow growth-rate and analyzing the ex vitro acclimatization. seeds were germinated in vitro in murashige and skoog (ms) medium, with macronutrients reduced to 50% (MS/2). After three months the plants were transferred to flasks of new same medium and kept in two germination chambers with the temperature adjusted to 15 °c and to 28 °c. After 24 months the plants were subject to biometric, photosynthetic pigments content and anatomical analyses. Results showed that plants maintained at 15 °c were smaller than those at 28 °c. nevertheless, there were no alterations in pigments content, anatomy. in both treatments there was a survival rate of 100%. This work showed that plants of this species can be kept in vitro at 15 °c with the aim of forming a slow-growth collection, thereby seeking its preservation, and can be transferred to growth at ex vitro condition to achieved 100% survival rate.key words -anatomy, Bromeliaceae, conservation, growth, photosynthetic pigments RESUMO − (Cultivo in vitro em temperatura baixa e aclimatização ex vitro de Vriesea inflata, uma bromélia ornamental). o cutivo in vitro iniciado a partir de sementes pode ser utilizado para preservação de espécies ameaçadas porque contribui para o fornecimento de um grande número de plantas com diversidade genética. Vriesea inflata (Wawra) Wawra, uma bromélia ornamental, é extensivamente e ilegalmente coletada da natureza, sendo importante sua preservação. Por meio do cultivo in vitro é possível formar coleções de plantas ameaçadas, sob crescimento lento, ocupando pouco espaço, com redução dos custos de manutenção das culturas. Este trabalho avaliou a influência da temperatura sobre o crescimento e morfologia de plantas de V. inflata mantidas in vitro, com objetivo de diminuir o crescimento, além de verificar a aclimatização destas para a condição ex vitro. sementes de V. inflata foram germinadas utilizando-se o meio de murashige and skoog (ms) com macronutrientes reduzidos à metade (MS/2). Após três meses as plantas foram transferidas para frascos contendo o mesmo meio e mantidos em câmaras de germinação, com temperaturas ajustadas para 15 °c e 28 °c. Após 24 meses essas plantas foram submetidas à analises biométricas, anatômicas e de pigmentos fotossintéticos. Verificou-se que as plantas mantidas a 15 °C foram menores qu...

show abstract

In vitro minimum growth for conservation of Drosophyllum lusitanicum

Cited by 25 publications

References 18 publications

In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions

In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions

Conservación de plantas regeneradas in vitro y análisis de la variación somaclonal de Cinchona officinalis, Linneo

In vitro culture at low temperature and ex vitro acclimatization of Vriesea inflata an ornamental bromeliad

Contact Info

Product

Resources

About