In vitro models to study human gut-microbiota interactions: Applications, advances, and limitations

Qi, Yong; Yu, Leilei; Tian, Fengwei; Zhao, Jianxin; Zhai, Qixiao

doi:10.1016/j.micres.2023.127336

Cited by 20 publications

(9 citation statements)

References 125 publications

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“…The in vitro cultivation of a cell monolayer to test with intestinal microbes is easy to obtain, inexpensive, reproducible, and provides raw results for preliminary studies. Caco-2, HT-29-MTX, T84, LS174T, and CCD 841 CoN are the most used cell lines for gastrointestinal in vitro models due to their specific properties [ 21 ]. For instance, Caco-2 and HT-29 cells, the latter able to secrete mucus, were co-cultured in the presence of enterotoxigenic E. coli (ETEC) H10407 to investigate the role of mucus in inducing and shaping ETEC gene expression [ 22 ].…”

Section: Animal Models or In Vitro Models For Gut Microbiota Research...mentioning

confidence: 99%

“…In addition to in vitro models consisting of cells and microbes, increasingly technological devices have been developed to approach the complexity of the human intestine. Among these, Transwell, Human Oxygen Bacteria Anaerobic (HoxBan), Host–Microbiota Interaction (HMI), TNO In vitro Model (TIM-1), TNO In vitro Model of the Colon (TIM-2), Human–Microbiota Crosstalk (HuMiX), and Simulator of Human Intestinal Microbial Ecosystem (SHIME) are just some of the systems created in the last decades following advances in the fields of bioengineering, millifluidics, and microfluidics [ 21 ]. Modern artificial devices are currently being used in numerous in vitro studies focused on the gut microbiota.…”

Section: Animal Models or In Vitro Models For Gut Microbiota Research...mentioning

confidence: 99%

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Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota

Calvigioni,

Mazzantini,

Celandroni

et al. 2023

Microorganisms

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Examining the interplay between intestinal pathogens and the gut microbiota is crucial to fully comprehend the pathogenic role of enteropathogens and their broader impact on human health. Valid alternatives to human studies have been introduced in laboratory practice to evaluate the effects of infectious agents on the gut microbiota, thereby exploring their translational implications in intestinal functionality and overall health. Different animal species are currently used as valuable models for intestinal infections. In addition, considering the recent advances in bioengineering, futuristic in vitro models resembling the intestinal environment are also available for this purpose. In this review, the impact of the main human enteropathogens (i.e., Clostridioides difficile, Campylobacter jejuni, diarrheagenic Escherichia coli, non-typhoidal Salmonella enterica, Shigella flexneri and Shigella sonnei, Vibrio cholerae, and Bacillus cereus) on intestinal microbial communities is summarized, with specific emphasis on results derived from investigations employing animal and in vitro models.

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Section: Animal Models or In Vitro Models For Gut Microbiota Research...mentioning

confidence: 99%

Section: Animal Models or In Vitro Models For Gut Microbiota Research...mentioning

confidence: 99%

Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota

Calvigioni,

Mazzantini,

Celandroni

et al. 2023

Microorganisms

View full text Add to dashboard Cite

show abstract

“…These metabolites can have different biological activities compared to the original compounds, and some may have even stronger health-promoting effects ( Santhiravel et al., 2022 ). In vitro models of the human gastrointestinal tract are key tools to simulate normal conditions, and study how these phytochemicals interact with gut microbes ( Qi et al., 2023 ). Additionally, the utility of such models can be extended when allied with techniques such as high-throughput sequencing to discern the genetic basis of underpinning mechanisms of different phytochemicals.…”

Section: Soil- and Gut Microbes And Functional Diversity Conceptmentioning

confidence: 99%

More than a meat- or synthetic nitrogen fertiliser-substitute: a review of legume phytochemicals as drivers of ‘One Health’ via their influence on the functional diversity of soil- and gut-microbes

Duarte,

Iannetta,

Gomes

et al. 2024

Front. Plant Sci.

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Legumes are essential to healthy agroecosystems, with a rich phytochemical content that impacts overall human and animal well-being and environmental sustainability. While these phytochemicals can have both positive and negative effects, legumes have traditionally been bred to produce genotypes with lower levels of certain plant phytochemicals, specifically those commonly termed as ‘antifeedants’ including phenolic compounds, saponins, alkaloids, tannins, and raffinose family oligosaccharides (RFOs). However, when incorporated into a balanced diet, such legume phytochemicals can offer health benefits for both humans and animals. They can positively influence the human gut microbiome by promoting the growth of beneficial bacteria, contributing to gut health, and demonstrating anti-inflammatory and antioxidant properties. Beyond their nutritional value, legume phytochemicals also play a vital role in soil health. The phytochemical containing residues from their shoots and roots usually remain in-field to positively affect soil nutrient status and microbiome diversity, so enhancing soil functions and benefiting performance and yield of following crops. This review explores the role of legume phytochemicals from a ‘one health’ perspective, examining their on soil- and gut-microbial ecology, bridging the gap between human nutrition and agroecological science.

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“… 15 , 16 Currently, different in vitro models are available to study intestinal epithelial infections with varying levels of complexity and translatability. 17 Transwell co-cultures based on Caco-2 cell lines have been widely used to study intestinal host-microbe interactions. 18 , 19 The CaCo-2 monolayer, originated from a colon adenocarcinoma, can spontaneously differentiate to resemble a small intestine-like phenotype and thereby, mimics important properties of the intestinal epithelium.…”

Section: Introductionmentioning

confidence: 99%

Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models

Nanlohy,

Johannesson,

Wijnands

et al. 2024

iScience

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In vitro models to study human gut-microbiota interactions: Applications, advances, and limitations

Cited by 20 publications

References 125 publications

Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota

Animal and In Vitro Models as Powerful Tools to Decipher the Effects of Enteric Pathogens on the Human Gut Microbiota

More than a meat- or synthetic nitrogen fertiliser-substitute: a review of legume phytochemicals as drivers of ‘One Health’ via their influence on the functional diversity of soil- and gut-microbes

Exploring host-commensal-pathogen dynamics in cell line and organotypic human intestinal epithelial models

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