In vitro mutagenesis and identification of mutants via ISSR in lily (Lilium longiflorum)

Abstract

An efficient in vitro mutagenesis protocol for Lilium longiflorum Thunb. cv. White fox has been established. The effect of 6-BA and NAA on adventitious bud formation from the bulblet-scale thin cell layers was tested. Results showed that the optimal medium for adventitious bud induction is MS basal medium supplemented with 2.0 mg/l 6-BA and 0.1 mg/l NAA. The differentiation frequency and the average number of adventitious buds reached 95.55% and 3.00, respectively. Various doses (0.0, 0.5, 1.0, 1.5, 2.0, and 2.5 Gy) of gamma rays were applied to investigate the effect of radiation on adventitious bud formation from bulblet-scale thin cell layers. The forming capacity of the adventitious buds significantly decreased with the increase of radiation dose. The results suggested that the optimal irradiation dose is 1.0 Gy. Dose of 1.0 Gy treatment resulted in 55.33% survival of irradiated bulblet-scale thin cell layers and 39.27% mutagenesis rate. The genetic variations among the morphological mutants were evaluated by DNA fingerprinting using ISSR molecular marker. The genetic variation frequency reached 36.06% using seven ISSR primers. Out of the 50 mutant lines transferred to the greenhouse, 9 were observed to have significantly different morphological characters than those of the controls.

Genetic Diversity in Tuberose (Polianthes tuberosa L.) Germplasm using Inter Simple Sequence Repeat (ISSR) Markers

“…Similar findings have been reported by Zietkiewicz et al, (1994). ISSR markers were proved to be useful in genetic diversity studies in other floricultural crops like chrysanthemum (Cai-hong et al, 2010;Baliyan et al, 2014), lilium (Guo et al, 2011;Xi et al, 2012;Zhao et al, 2014) and Zinnia (Ye et al, 2008).…”

Field Evaluation and Molecular Analysis of Three Early Flowering Canola Mutants under Natural Salinity-Stressed Environment

“…This study showed that the mutants and their parent and the check possess a moderate polymorphism as well as revealed by Xi et al (2012) and Wang et al (2017) who also detected a moderate polymorphism among gamma rayinduced mutants. The appearance or disappearance of bands as specific for γ-ray induced mutants is may be attributed to DNA structural rearrangements caused by different types of DNA damages (Selvi et al, 2007 andMejri et al, 2012).…”

Dwarfing of Breadfruit (Artocarpus altilis) Trees: Opportunities and Challenges

“…[97][98][99], streptomycin-resistance in Solanum surattense [100], salt tolerance in Chrysanthemum morifolium [101] and various morphological characters in Lilium longiflorum [102]. The techniques have successfully been used to improve agronomic characters such as tree stature, fruit yield and disease resistance of a variety of fruit crops (Table 1), including selection of 98 dwarf clones from 10,000 mutant banana plants [103].…”