In Vitro Novel Study the Effect of Scopoletin Compound and Silver Nanoparticles of Fenugreek Extract on Focal Adhesion Kinase Enzyme as Anti-Metastatic in Serum of Iraqi Patients of Stage ιι and Stage ιιι with Pancreatic Cancer

Abstract

Background: Experts consider pancreatic cancer one of the most deadly types. For unknown reasons, pancreatic cancer incidence and mortality are rising. Scopoletin and Ag-NPs compounds have therapeutic properties and are utilized experimentally in vitro as chemotherapies for numerous disorders.Objective: This study manufactures silver nanoparticles from fenugreek extract and analyzes their anti-metastatic effects with scopoletin at different doses to find the best combination to lower FAK enzyme concentration. Patient and Methodes: Sixty pancreatic cancer serum samples were divided: 30-Stage II patient samples (male and female, 45-65 years old), 30 stage IV patients of both sexes and ages (52-79), Thirty serum samples from healthy 23-45-years-olds served as a control group. Silver nanoparticles and scopoletin solutions were tested to find the appropriate concentration to reduce the focal adhesion kinase (FAK) enzyme. Resuts: Researchers showed that patients with stage ΙΙ and stage ΙΙΙ with pancreatic cancer and the control group had enzyme concentrations of 1.620.11ng/ml, 1.790.06 ng/ml, and 0.940.41 ng/ml, respectively, when treated with a 2 ppm solution of nanoparticles. Stage ΙΙ and stage ΙΙΙ with pancreatic cancer patients had lower enzyme concentrations than controls (1.570.15 ng/ml and 1.410.07 ng/ml). when treated with a 8 ppm solution of Scopoletin solution.Conclusion: Based on their potential to decrease focal adhesion kinase enzyme concentrations in the blood serum of patients with stageΙΙ and stageΙΙΙ Iraqi pancreatic cancer, scopoletin and silver nanoparticles of fenugreek can be recommended as anticancer materials.