In vitro potential anthelmintic activity of Biophytum petersianum on Haemonchus contortus

Sambodo, Priyo; Prastowo, Joko; Kurniasih, Kurniasih; Indarjulianto, Soedarmanto

doi:10.14202/vetworld.2018.1-4

Cited by 25 publications

(24 citation statements)

References 14 publications

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“…Protein profile of AF of infected NDV embryo of ECEs was analyzed by SDS-PAGE test. A total of 5 μl of AF was introduced into 7.5% stacking gel and 12% separating gel, then electrophoresis was run at room temperature with a voltage of 200 V. After electrophoresis, the gel was stained using a 0.1% Coomassie blue solution for 24 h and destained using methanol and glacial acetic acid for approximately 3 h [ 14 , 15 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Newcastle disease virus level of infection on embryonic length, embryonic death, and protein profile changes

Qosimah¹,

Murwani²,

Sudjarwo³

et al. 2018

Vet World

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Background and Aim:Newcastle disease virus (NDV) is an obligate intracellular parasite. Virus can only live on living cells. The embryonated chicken eggs (ECEs) are one of the growth media of virus that is a cheap, easy to do, and accurate for showing patterns of virus change in the host. Higher virus titers indicate the higher number of viruses and more virulent to infect host. This research aimed to investigate the effect of different level of NDV titer infection in ECEs on protein profile, embryonic length, mortality, and pathological change.Materials and Methods:The study used a completely randomized design of six treatments and seven replications. The treatments were different level of NDV titer infection in allantoic fluid (AF) of 9-11 days ECEs, i.e., P1=20, P2=26, P3=27, P4=28, P5=29, and P6=210 hemagglutination unit (HAU). All samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Data were analyzed using one-way ANOVA with p=0.05 for length of the embryo and descriptive analysis for embryo mortality, pathology change, and protein band.Results:The result showed that protein profile of NDV-infected ECEs of all different levels is more complex than protein profile of no NDV-infected ECEs. NDV infected of all different levels showed longer size embryo, higher mortality embryo at the first 2 days, and higher occurrence of hemorrhagic in all part of bodies of embryo than those of no NDV infected.Conclusion:It was concluded that NDV infection of all different level decreased health conditions of chicken embryo of ECEs of 9-11 days old. Different level of NDV infection of ECEs of 9-11 days old showed no significantly different embryo profiles. However, all of the NDV-infected embryos were shorter, death on the 2nd day, and suffered more hemorrhage on all body surfaces than uninfected NDV embryos.

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Section: Methodsmentioning

confidence: 99%

Effect of Newcastle disease virus level of infection on embryonic length, embryonic death, and protein profile changes

Qosimah¹,

Murwani²,

Sudjarwo³

et al. 2018

Vet World

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“…Sambodo et al . [ 36 ] added that cuticle changes due to interactions with aqueous extract of Biophytum petersianum , where the part has an important role in the motility and absorption of nutrients.…”

Section: Discussionmentioning

confidence: 99%

In vitro anthelmintic activity of aqueous and ethanol extracts of Paraserianthes falcataria bark waste against Haemonchus contortus obtained from a local slaughterhouse in Indonesia

2020

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Aim: This study was conducted to determine the anthelmintic activity of aqueous and ethanol extracts of Paraserianthes falcataria bark against Haemonchus contortus. Materials and Methods: Ethanol extract of bark (E.E.B.) waste and aqueous extract of bark (A.E.B.) waste of P. falcataria (at concentrations 0, 0.2, 0.4, 0.8, 1, 2.5, and 5%) and albendazole (2 mg/ml) as the positive control were placed in separate Petri dishes (50 mm). Twenty H. contortus worms were placed in Petri dishes and incubated at 37°C for 0.5, 1, 2, 3, 4, 5, 6, and 12 h. Mortality of each worm was ensured by pressing the body of the worm with a pair of tweezers and keeping it in lukewarm water for 5 min before declaring it dead. Mortality is defined as amount of death individuals and time of mortality of each worm was recorded. The parasites were then observed using scanning electron microscopy (SEM) at an accelerating voltage of 15 Kv. Statistical analysis was performed using SPSS 21.0 software, two-way ANOVA followed by Tukey's test to detect significant differences (p<0.05). The result was expressed as the mean ± standard deviation. Results: The E.E.B. and A.E.B. of P. falcataria contained active compounds, such as tannin, alkaloid, flavonoid, saponin, steroid, and triterpenoid. E.E.B. had a higher content of phenol, while A.E.B. had a higher content of flavonoid. In this study, P. falcataria showed a significant effect (p=0.00) on H. contortus in vitro. E.E.B. (0.8%) was able to exterminate H. contortus completely after 6 h, more effective than A.E.B. (5%) while the positive control requires (2 mg/ml) after 2 h. SEM analysis of the worm treated with E.E.B. and A.E.B. showed damaged cuticle structure. Conclusion: The aqueous and ethanol extracts of P. falcataria bark waste demonstrated anthelmintic activity against H. contortus.

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“…The cuticle change with the longitudinal and transverse wrinkles after in vitro exposure to Biophytum persianum rich in condensed tannin on H. contortus was evaluated by Sambodo et al . [ 16 ] crude extract. The wrinkles in the cuticle and anterior end of H. contortus were also observed by Martinez-Ortiz-de-Montellano et al .…”

Section: Discussionmentioning

confidence: 99%

Morphology and morphometry of Haemonchus contortus exposed to Gigantochloa apus crude aqueous extract

et al. 2018

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Aim:Haemonchus contortus is the most pathogenic nematode infesting the digestive tract of goats and sheep worldwide leading to a tremendous loss in a variety of routes. Economic losses due to haemonchosis in subtropic and tropic areas are usually caused by poor weight gain, minimized growth, loss of production, and mortality. The prevalence of haemonchosis in Indonesia is 89.4% in goat, and annual loss achieved 1 million US dollars. This study evaluated in vitro effects of Gigantochloa apus crude aqueous extract as an anthelmintic on H. contortus morphology and morphometry.Materials and Methods:Bligon goats which are naturally infected were collected from slaughtered goat from local slaughterhouses, namely Besi Sleman. Bligon goat’s abomasum part was carefully examined and transported to the Parasitology Laboratory, University of Gadjah Mada, Yogyakarta. H. contortus was obtained from 4 to 6-month-old female goat from slaughterhouses in Yogyakarta area. H. contortus was collected from abomasum and put into a Petri dish containing 0.62% water saline. The number of H. contortus used for each concentration is 25. H. contortus was soaked in each concentration for 4 h. The figure of the parasites or parts of parasites was captured using camera Lucida, and they were measured using both objective micrometer and objective ocular micrometer. All the capturing processes were done with the help of Olympus Digital Camera under Olympus CX21 microscopic. Parasite morphology was identified in morphological and morphometric characters.Result:Morphology of H. contortus revealed the cervical papillae bulge appears unclear shape and anterior end is more tapered. Vulvar flab control is not tapered, but vulvar flab which gets aware of G. apus crude aqueous extract looks more pointed. The gubernaculum appears irregular compared to gubernaculum control which tends to be more compact, and the posterior end form appears irregular more than posterior end control. Morphometry study of H. contortus indicates that it has a significant difference for body length, body width, cervical papillae, and spicule length in the male.Conclusion:G. apus crude aqueous extract activity revealed morphology change such as cervical papillae, vulvar flab, gubernaculum, posterior end, and reduced morphometry measurement of H. contortus adult worms, notably in body length, body width, cervical papillae width, gubernaculum, and spicule length in males and body length, body width, cervical papillae width, and vulva length in females.

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In vitro potential anthelmintic activity of Biophytum petersianum on Haemonchus contortus

Cited by 25 publications

References 14 publications

Effect of Newcastle disease virus level of infection on embryonic length, embryonic death, and protein profile changes

Effect of Newcastle disease virus level of infection on embryonic length, embryonic death, and protein profile changes

In vitro anthelmintic activity of aqueous and ethanol extracts of Paraserianthes falcataria bark waste against Haemonchus contortus obtained from a local slaughterhouse in Indonesia

Morphology and morphometry of Haemonchus contortus exposed to Gigantochloa apus crude aqueous extract

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