In vitro potential effect of Pipper longum methanolic extract against protoscolices of hydatid cysts

Cheraghipour, Kourosh; Beiranvand, Marjan; Zivdari, Masoomeh; Amiri, Sana; Masoori, Leila; Nourmohammadi, Morteza; Ali, Ali Safar Maken; Abbaszadeh, Sepideh; Moradpour, Kobra; Marzban, Abdolrazagh

doi:10.1016/j.exppara.2020.108051

Cited by 11 publications

(5 citation statements)

References 33 publications

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“…A large number of plant extracts were tested in vitro on E. granulosus protoscoleces [9,[37][38][39][40]; however, only a few of them were investigated for preventive or therapeutic activities in the search for new alternative treatments for CE [41][42][43][44][45].…”

Section: Discussionmentioning

confidence: 99%

Antiparasitic Effects of Asteraceae Species Extracts on Echinococcus granulosus s.s

Albani

Borgo

Fabbri

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Cystic echinococcosis is a zoonotic disease caused by the parasite Echinococcus granulosus sensu lato (s.l.), which is worldwide distributed and causes long-lasting infections in animals and humans. The existing treatment is limited to the use of benzimidazoles, mainly albendazole (ABZ). However, it has unwanted side effects and its efficacy is about 50%. The Asteraceae family includes plants that have therapeutic applications (medicinal species) and has an important role in new drug development. The species belonging to a different genus of this family show a wide range of anti-inflammatory, antimicrobial, antioxidant, hepatoprotective, and antiparasitic activities, among others. The aim of the present study was to evaluate the in vitro efficacy of extracts of four Asteraceae species against protoscoleces of E. granulosus sensu stricto (s.s.). On the other hand, the Stevia aristata extract was assessed on the murine cyst of E. granulosus (s.s.) and the efficacy of S. aristata extract was investigated in a murine model of CE. Stevia satureiifolia, S. aristata, Grindelia pulchella, and G. chiloensis extracts at 100 μg/mL caused a decrease in protoscoleces viability; however, S. aristata extract produced the greatest in vitro protoscolicidal effect. After 20 days of treatment with the highest concentration (100 μg/mL) of S. aristata extract, protoscoleces viability decreased to 0%. The tegumental changes observed by scanning electron microscopy were consistent with the reduction in vitality. The collapse of the germinal layer was registered in 60 ± 5.8% and 83.3 ± 12.0% of cysts treated during 4 days with 50 and 100 μg/ml, respectively. The half maximal effective concentration (EC50) value of the S. aristata extract against E. granulosus (s.s.) cysts was 47.86 μg/mL (96 h). The dosage of infected animals with the 50 mg kg−1 dose of S. aristata extract resulted in a significant reduction in cyst weight in comparison with the control group. In conclusion, S. aristata extract was demonstrated to exert a marked effect, both in vitro and in the murine model.

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Section: Discussionmentioning

confidence: 99%

Antiparasitic Effects of Asteraceae Species Extracts on Echinococcus granulosus s.s

Albani

Borgo

Fabbri

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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“…In this context, many plant extracts have shown promising in vitro results on the viability of E. granulosus s.l. protoscoleces (Al-Abodi et al ., 2019; Tabatabaei et al ., 2019; Ali et al ., 2020; Cheraghipour et al ., 2021). However, only a few plant extracts have been tested on the E. granulosus s.l.…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic activity ofStevia multiaristataextract againstEchinococcus granulosus sensu stricto

et al. 2021

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Cystic echinococcosis is a zoonotic disease caused by the larval stage of the parasite Echinococcus granulosus sensu lato. The available anti-parasitic treatment is mostly limited to a continuous administration of albendazole. However, due to its numerous side-effects and efficacy of around 50%, there is a need to find new drugs to improve the treatment for this disease. In the current study, the in vitro and in vivo efficacy of a Stevia multiaristata extract against E. granulosus sensu stricto (s.s.) was demonstrated. Stevia multiaristata extract (100 and 50 μg mL−1) caused a quick viability decrease on protoscoleces which was consistent with the observed tegumental alterations. Loss of turgidity was detected in 95 ± 3.4% of cysts incubated with S. multiaristata extract during 2 days (100 μg mL−1) and the collapse of the germinal layer was observed in 60 ± 9.3% of cysts treated with 100 μg mL−1 of the S. multiaristata extract during 4 days. The half maximal effective concentration value was 69.6 μg mL−1 and the selectivity index for E. granulosus s.s. cysts was 1.9. In this clinical efficacy study, the treatment of infected mice with the S. multiaristata extract (50 mg kg−1) caused a significant decrease in the weight of the cysts compared with the control group. These results coincided with the tissue damage observed in the cysts at the ultrastructural level. In conclusion, we observed high protoscolicidal and cysticidal effects, and significant reduction in the weight of the cysts in experimentally infected mice following treatment with the S. multiaristata extract.

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“…Methanolic extract of P. longum exhibited antiparasitic activity against protoscolices of hydatid cysts of Echinococcus granulosus . This is the pioneer demonstration of scolicidal activity of P. longum (Cheraghipour et al, 2021).…”

Section: Pharmacological Activitiesmentioning

confidence: 96%

Piper longum L.: A comprehensive review on traditional uses, phytochemistry, pharmacology, and health‐promoting activities

Biswas

Ghorai

Mishra

et al. 2022

Phytotherapy Research

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Piper longum (family Piperaceae), commonly known as "long-pepper" or "Pippali" grows as a perennial shrub or as an herbaceous vine. It is native to the Indo-Malaya region and widely distributed in the tropical and subtropical world including the Indian subcontinent, Sri Lanka, Middle-East, and America. The fruits are mostly used as culinary spice and preservatives and are also a potent remedy in various traditional medicinal systems against bronchitis, cough, cold, snakebite, and scorpion-sting and are also used as a contraceptive. Various bioactive-phytochemicals including alkaloids, flavonoids, esters, and steroids were identified from the plant extracts and essential oils from the roots and fruits were reported as antimicrobial, antiparasitic, anthelminthic, mosquito-larvicidal, antiinflammatory, analgesic, antioxidant, anticancer, neuro-pharmacological, antihyperglycaemic, hepato-protective, antihyperlipidaemic, antiangiogenic, immunomodulatory, antiarthritic, antiulcer, antiasthmatic, cardioprotective, and anti-snake-venom agents. Many of its pharmacological properties were attributed to its antioxidative and antiinflammatory effects and its ability to modulate a number of signalling pathways and enzymes. This review comprehensively encompasses information on habit, distribution, ethnobotany, phytochemistry, and pharmacology of P. longum in relation to its medicinal importance and health benefits to validate the traditional claims supported by specific scientific experiments. In addition, it also discusses the safety and toxicity studies, application of green synthesis and nanotechnology as well as clinical trials performed with the plant also elucidating research gaps and future perspectives of its multifaceted uses.

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In vitro potential effect of Pipper longum methanolic extract against protoscolices of hydatid cysts

Cited by 11 publications

References 33 publications

Antiparasitic Effects of Asteraceae Species Extracts on Echinococcus granulosus s.s

Antiparasitic Effects of Asteraceae Species Extracts on Echinococcus granulosus s.s

Anthelmintic activity ofStevia multiaristataextract againstEchinococcus granulosus sensu stricto

Piper longum L.: A comprehensive review on traditional uses, phytochemistry, pharmacology, and health‐promoting activities

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