In Vitro Preliminary Phytochemical Screening and Free Radical Scavenging Ability of Drosera indica L.

Koirala

Cell Mol Biol (Noisy-le-grand)

et al. 2021

Reinwardtia indica is traditionally used for wound healing. The main aim of this study was to evaluate the wound healing activity of leaves extracts of R. indica using the excision wound model in rats. The leaves of R. indica were collected from Gondrang, Chitwan, Nepal. Leaves were shade dried, extracted by double maceration and subjected to phytochemical screening. Then, the fusion method was used for the formulation of ointment and evaluated. Rats (n=24) were divided into four groups with 6 in each. Excision wound model was used, 2 cm diameter (314 mm 2 ), 2 mm depth wound was created. The treatment was given daily topically to all groups and the % mean wound contraction rate was calculated on days 4, 8, 12 and 16. The result was analyzed statistically using Graph pad prism version 5. Phytochemical test revealed the presence of alkaloid, flavonoid, tannin, phenol, terpenoid, carbohydrate, etc. All the evaluation parameters showed satisfactory results. The extract of R. indica ointment (2% w/w and 5% w/w) increased the wound contraction rate day by day. The % means wound contraction rate, on day 12, (80% and 88%), and on day 16, (97% and 100%) and statistically significant difference was at p<0.0001. The R. indica extract ointment showed an increased wound contraction rate. So, in further R. indica could be used for commercial production of wound healing ointment.

Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Reinwardtia indica: phytochemical screening and evaluation of wound healing activity of the extracts in experimental model rats

Koirala

Cell Mol Biol (Noisy-le-grand)

et al. 2021

“…Various sensory parameters of the plant material (such as colour, odour, size, shape, and taste) were studied by organoleptic evaluation [27].…”

Section: Organoleptic Evaluationmentioning

confidence: 99%

Standardization of Ayurvedic Drug- Nyctanthes arbor-tristis, Hippophae salicifolia, Ocimum tenuiflorum and Reinwardtia indica and Combined Herb-Herb

Kumari

Dubey²

2020

Nepal J Biotechnol

The herbal medicines have reached extensive acceptability as therapeutic agents for various clinical diseases due to global demand. Therefore, standardization is the essential and initial step to drug development. It is for the establishment of consistent biological activity, a consistent chemical profile and biomarker identification. It improves the safety and efficacy of herbal medicine to provide the best herbal medicine to society and increase popularity rather than non-standardized extracts. In addition, it is essential to practice or maintain a quality assurance program for the production and manufacturing of herbal medicine that includes the basis of organoleptic characters and photomicrographs, physicochemical, proximate analysis phytochemical evaluation and quality control analysis and order to assess the quality of drugs, based on the concentration of their active principles. WHO has provided specific guidelines for the assessment of the safety, efficacy and quality of herbal drugs as a prerequisite for global harmonization and of utmost importance. In the present study, the herbal extracts were cleaned, dried in the shade and powdered by passing through the sieve as per the method described in the standard protocol. An overview covering various techniques employed in the extraction and characterization of Nyctanthes arbortristis, Hippophae salicifolia, Ocimum tenuiflorum and Reinwardtia indica, standardization is reported in this study. The obtained data would be very significant for future clinical aspects, as the bioactive molecules present in the extracts may exhibit synergistic effect with other bioactive compound and show a better therapeutic value. Thus, this study provides standardized and therapeutically potential data of active polyherbal formulations for the different ailments.

“…It is used in traditional Chinese medicine formulation for the treatment of acute or chronic gastritis (Cn, 2014a;2014b). Reinwardtia trigyna is used as natural herbicide and weedicide as it showed a good phytotoxic activity (Khan et al, 1998;Perveen, Qaiser, 2008;Abha, Swati, Shukla, 2013). The reported pharmacological activity of the other plants belong to the same family (flax family) are used as antioxidant, wound healing, anti-microbial, anti-inflammatory, and anti-nociceptive agent.…”

Section: Introductionmentioning

confidence: 99%

Toxicity assessment of the alcoholic leaves extract of Reinwardtia indica

Upadhyay

Shukla

Tiwari

et al. 2019

Braz. J. Pharm. Sci.

The present study aimed to evaluate the safety of the alcoholic leaves extract of Reinwardtia indica in Charles foster rats through an acute and sub-acute oral administration.For assessment of acute oral toxicity test, ratswere orally treated with single dose of the alcoholic leaves extract of Reinwardtia indica at the doses of 50, 250, 500, 1000 2000 and 5000 mg/kg. In sub-acute toxicity study, using the OECD guidelines no. 407, the extract was administered at the doses of 50, 250, 500, 1000, 2000 mg/kg/day for 28 consecutive days and at the dose of 2000 mg/kg satellite group also used for 6 weeks.In acute toxicity above mentioned doses neither showed mortality nor exterior signs of toxicity. In sub-acute, study no significant changes found in haematological and biochemical level ofthe treated rat after 14 days and 28 days in comparison to control. The histopathology of rat brain, kidney, liver, and heart also showed the no cellular changes after extract treated rat.The alcoholic leaves extract of Reinwardtia indica was found non-toxic in single drug dose administration up to 5000 mg/kg (acute study) and in sub-acute administration up to 2000 mg/kg.