In Vitro Probiotic Properties and DNA Protection Activity of Yeast and Lactic Acid Bacteria Isolated from A Honey-Based Kefir Beverage

Abstract

The probiotic characteristics of three acid-tolerant microbial strains, viz., Lactobacillus satsumensis LPBF1, Leuconostoc mesenteroides LPBF2 and Saccharomyes cerevisiae LPBF3, isolated from a honey-based kefir functional beverage, were studied following the requirements established by the Food and Agriculture Organization of the United Nation/World Health Organization (FAO/WHO), including host-associated stress resistance, epithelium adhesion ability, and antimicrobial activity. The three microbial strains tolerated different pH values (2.0, 3.0, 4.0 and 7.0) and bile salt concentrations (0.3% and 0.6%), and survive in the presence of simulated gastric juice, which are conditions imposed by the gastrointestinal tract. In addition, they showed high percentages of hydrophobicity, auto aggregation and anti-pathogenic against Escherichia coli and Staphylococcus aureus, with no hemolytic activity. The protective capacity of human DNA through microbial treatment was investigated by single-cell gel electrophoresis (SCGE) comet assay. The three selected strains showed DNA protection effect against damage caused by hydroxyl radical (H2O2). However, when the S. cerevisiae treatment was applied, the most effective DNA protection index was observed, which can be associated to its high production of extracellular antioxidants as reveled by the 2,2-diphenyl-1-picryl-hydrazylhydrate (DPPH) method. These results indicated that the three selected microbial strains could be useful for preventing oxidative DNA damage and cellular oxidation in food products. As well-adapted microbial cells, the selected strains can be used for production of non-dairy functional beverages, especially for vegans and/or vegetarians and lactose intolerants.

Oxidative stress tolerance and antioxidant capacity of lactic acid bacteria as probiotic: a systematic review

“…191 The prevention of oxidative stressinduced DNA damage by Lactobacillus has been evaluated in different mammalian cells (Caco-2 cells, HCT 116 cells, HT-29 cells, and lymphocytes) using a single-cell gel electrophoresis assay (Comet assay), which was developed based on DNA migration. [192][193][194][195] The DNA-protective capacity of probiotic LAB can also be detected by molecular biology techniques to estimate oxidative stress protection. 196 Nowak and colleagues used the DNA repair enzymes endonuclease III and formamidopyrimidine-DNA glycosylase to evaluate the antioxidant capacity of Lactobacillus toward H 2 O 2 and several human carcinogens based on the recognition of oxidized DNA bases by DNA repair enzymes.…”

Selection of Potential Yeast Probiotics and a Cell Factory for Xylitol or Acid Production from Honeybee Samples

“…Some strains (CNCM I-745 and OBS2) of Saccharomyce s genus survive through the intestinal tract and are also used industrially as dietary supplements because they contain high mineral content and nutrients [ 4 , 5 ]. To restore and maintain the natural biota in the gastrointestinal tract, probiotic yeast is used as a nutritional supplement, for example in Kefir milk [ 6 , 7 ], cereal-based fermented beverage [ 8 ], Red yeast rice [ 9 ], maize-based cereals [ 10 ] etc. Reductions in symptoms of acute diarrhea and related diseases are observed on consuming S. boulardii and S. cerevisiae used as pharmaceutical products [ 11 , 12 ].…”

Selected Kefir Water from Malaysia Attenuates Hydrogen Peroxide-Induced Oxidative Stress by Upregulating Endogenous Antioxidant Levels in SH-SY5Y Neuroblastoma Cells

“…Although the antioxidant, anti-apoptotic, and neuroprotective activities of kefir water cultured in different parts of the world have been reported [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35], there is no study made on kefir water cultured in Malaysia for its neuroprotective effects in NDD. This study investigated the neuroprotective potential of six different kefir water samples cultured in Malaysia against H 2 O 2 -induced cytotoxic effects on differentiated human neuronal cells and studied their cellular and molecular morphological features to provide a better insight into how kefir provides its health benefits.…”