In vitro production of llama (Lama glama) embryos by intracytoplasmic sperm injection: Effect of chemical activation treatments and culture conditions

Sansinena, M.; Taylor, S.; Taylor, Paul J.; Schmidt, Edward E.; Denniston, R.S.; Godke, R.A.

doi:10.1016/j.anireprosci.2006.05.020

Cited by 33 publications

(25 citation statements)

References 51 publications

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“…Nevertheless, the reproduction of the Huacaya alpaca is limited by several adverse factors that lead to low rates of fecundity and a pregnancy percentage ranging from 40% to 60% (Díaz et al, ). Particular features of libido and copulation (Fernández Baca, ; Tibary & Vaughan, ), low semen quality with low sperm concentration (Giuliano et al, ), high percentage of morphological abnormal spermatozoa (Buendía et al, ) and a high viscosity of seminal plasma (Bravo, Ccallo, & Garnica, ) have limited the development of SAC production programmes (Giuliano et al, ) and in vitro production of embryos (Conde et al, ; Sansinena et al, ).…”

Section: Introductionmentioning

confidence: 99%

Sperm kinematic characterization of alpaca (Vicugna pacos L.) during the reproductive season

Meza

Caldeira

Valverde

et al. 2018

Reprod Domestic Animals

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Semen analysis is a key factor when determining the fertility ability in males. South American camelids, and in particular the alpaca, have been studied very little when compared with other farm animals. The aim of this work was to perform the kinematic characterization of alpaca spermatozoa collected directly from the deferent duct by using CASA-Mot (Computer Assisted Semen Analysis for Motility) technology. Samples were obtained every three days throughout the reproductive season during two periods and with a break of seven days in the middle. During both periods, the quality of the sample's motility and kinematics increased over the first two days and then subsequently decreased. This pattern was similar in all animals. It was concluded that the introduction of resting times can be useful to improve sperm quality for artificial insemination purposes in natural conditions.

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Section: Introductionmentioning

confidence: 99%

Sperm kinematic characterization of alpaca (Vicugna pacos L.) during the reproductive season

Meza

Caldeira

Valverde

et al. 2018

Reprod Domestic Animals

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“…36 Unlike in other species, semen liquefaction may take several hours (24 to 48 hours) or not occur at all. 25,[39][40][41] In addition, spermatozoa from these species show oscillatory movements in the ejaculate rather than progressive motility when the ejaculate is not treated. 25,[39][40][41] In addition, spermatozoa from these species show oscillatory movements in the ejaculate rather than progressive motility when the ejaculate is not treated.…”

Section: Semen Preparation Semen Characteristicsmentioning

confidence: 99%

“…[41][42][43] Because of these characteristics, the first attempt to produce llama embryos via IVF used epididymal sperm, which is not viscous, has a high motility, and is easy to manipulate. 40 Similarly, the only study of in vitro embryo production in alpacas was carried out using spermatozoa from the epididymus. 40 Similarly, the only study of in vitro embryo production in alpacas was carried out using spermatozoa from the epididymus.…”

Section: Semen Preparation Semen Characteristicsmentioning

confidence: 99%

“…25,40 Sansinena et al used a discontinuous gradient system in 4-(2-hydroxyethyl)-1piperazineethanesulfonic acid (HEPES)-buffered human tubal fluid (HTF) (1 mL of 50% over 1 mL of 90% and 0.5 mL extended semen; ISolate, Irvine Scientific, Santa Ana, CA), centrifuging at 400 g for 15 minutes. Moreover, it maximizes recovery of motile sperm, as the volume of the gradient, the centrifugation force, and time to the sample characteristics can be adjusted.…”

Section: Embryo Preservationmentioning

confidence: 99%

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Embryo Preservation and in Vitro Production of Embryos

Trasorras¹,

Giuliano²,

Miragaya³

2014

Llama and Alpaca Care

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“…Handling of camelid semen in the laboratory is difficult due to viscous nature of semen (Tibary and Vaughan, 2006;Del Campo et al, 1994;Maxwel et al, 2008;Sansinena et al, 2007) and is difficult to assess spermatozoal motility for further semen analysis and processing. For efficient cryopreservation, the sperm-cryoprotective and nutritive compounds ought to come in contact with sperm cell membrane during equilibration, therefore liquefaction of camelid ejaculates is necessary for improving postthawing semen parameters (Monaco et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Effect of different proteolytic enzymes on liquefaction of semen of dromedary camel

et al. 2018

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In most of domestic animals, semen biology is well studied, but it is not so well studied in camelids particularly in dromedary camels. In camelids, the ejaculated semen is highly viscous, so before its evaluation and processing for preservation, it is necessary to be liquefied. Our study was designed to evaluate the effect of three different enzymes (0.1% Collagenase, 0.5 X Accutase, 0.1% Trypsin-EDTA - Ethylene-diamine-tetra-acetic acid) on seminal viscosity at different time intervals along with its effects on seminal parameters. Semen was collected from the six adult healthy male camels using female camel as dummy. Bovine artificial vagina was used for the semen collection and ejaculate was kept at 37ºC for further processing after its collection. All three enzymes were diluted in 1x phosphate buffered saline (PBS) and the semen samples were kept at 37ºC. The samples were evaluated at different time intervals 0, 5, 10, 15, 30, 60, 120 and 240 min (T0–T240) for viscosity and it was evaluated by pipette method. Immediately after collection, the viscosity was very high, and no sperm mass motility was seen. However, after enzyme treatment viscosity was showed declining trend in all treatment groups compared to control. In trypsin treated group maximum motility was reached in 15 minutes. Live-dead percent was observed to be similar among treatment groups. Observations on liquefaction of semen revealed that use of enzymes decreasing the viscosity but with different rates.

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In vitro production of llama (Lama glama) embryos by intracytoplasmic sperm injection: Effect of chemical activation treatments and culture conditions

Cited by 33 publications

References 51 publications

Sperm kinematic characterization of alpaca (Vicugna pacos L.) during the reproductive season

Sperm kinematic characterization of alpaca (Vicugna pacos L.) during the reproductive season

Embryo Preservation and in Vitro Production of Embryos

Effect of different proteolytic enzymes on liquefaction of semen of dromedary camel

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