In vitro proliferation and differentiation of human bone marrow mesenchymal stem cells into osteoblasts on nanocomposite scaffolds based on bioactive glass (64SiO 2 -31CaO-5P 2 O 5 )-poly- l -lactic acid nanofibers fabricated by electrospinning method

Shams, Mehdi; Karimi, Mohammad; Ghollasi, Marzieh; Nezafati, Nader; Shahrousvand, Mohsen; Kamali, Mohammad; Salimi, Ali

doi:10.1016/j.msec.2017.02.165

Cited by 60 publications

(25 citation statements)

References 41 publications

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“…Figure (b) shows the SEM micrographs of the cells attached to the surface of TG2‐1.5B composite paste. As can be seen, the cells show optimally adherence to the surface and create false legs with multidimensional morphology showing the optimum surface topography of the composite paste for settlement and growth of the adherent cells . Figure (c,d) shows the optical micrographs of MG63 cells cultured on TG2‐1.5B composite paste.…”

Section: Resultsmentioning

confidence: 94%

Effects of GPTMS concentration and powder to liquid ratio on the flowability and biodegradation behaviors of 45S5 bioglass/tragacanth bioactive composite pastes

Rasti

Hesaraki

Nezafati

2019

J of Applied Polymer Sci

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Injectable composite pastes were prepared using melt‐derived 45S5 bioactive glass and tragacanth crosslinked by (3‐glycidyloxypropyl)trimethoxysilane (GPTMS). The effect of powder to liquid ratio (P:L = 1.0:2.0–1.0:2.5) and GPTMS/tragacanth ratio (0.0–1.5) on the injectability, swelling behavior, rheology, bioactivity, and cellular behavior of the pastes was investigated. Based on the results, the apparent stability and consistency of the pastes increased upon crosslinking by GPTMS. Due to the increased interactions between tragacanth and glass, a hysteresis loop with larger area was formed in the presence of GPTMS. With increase of GPTMS:tragacanth ratio from 0 to 1.5, the swelling percent dropped from 24.65 to 16.25% after 24 h and the degradation percent also went down from 27.89 to 9.11% after 21 days in the simulated body fluid. The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay showed a drop in the optical density of MG63 osteoblasts up to 30.07% after exposure to the GPTMS‐crosslinked composite pastes for 3 days. However, the number of viable cells gradually increased in the presence of the pastes and the cell morphology remained unchanged over time. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47604.

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Section: Resultsmentioning

confidence: 94%

Effects of GPTMS concentration and powder to liquid ratio on the flowability and biodegradation behaviors of 45S5 bioglass/tragacanth bioactive composite pastes

Rasti

Hesaraki

Nezafati

2019

J of Applied Polymer Sci

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“…The effect of Bioglass and its dissolution products on stem cells is less clear and there is some contradictory evidence in the literature. Osteogenic differentiation of human bone marrow stem cells (hMSCs) in contact with BGs was investigated [47][48][49][50][51][52][53], but results varied depending on the particular types of stem cells tested. Some articles found no effect on stem cells in vitro: Bioglass 45S5 did not stimulate the alkaline phosphatase (ALP) activity of hMSCs from five different human donors [54] and a Bioglass 45S5 coating on a polymeric substrate had no direct effect on osteoprogenitor cell differentiation or bone formation [55].…”

Section: Introductionmentioning

confidence: 99%

Human mesenchymal stem cells differentiate into an osteogenic lineage in presence of strontium containing bioactive glass nanoparticles

Naruphontjirakul

Tsigkou

et al. 2019

Acta Biomaterialia

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“…Through the formation of surface hydroxy carbonate apatite (HCA) layer, these ceramic bonds to both hard and soft tissues (Lizzi et al, 2017;Salinas & Vallet-Regí, 2013;Siqueira et al, 2017). Taking into account the previous art, bioactive glass ceramic affects the cell adhesion, proliferation, differentiation and colonization on the surface of implants (Kargozar et al, 2017;Shamsi et al, 2017;Wu, Wu, Xue, Li, & Liu, 2017;Wu, Zhou, Chang, & Xiao, 2013). Various composite hydrogels based on the bioactive glass have been synthesized recently, using biodegradable natural polymers including:…”

Section: Introductionmentioning

confidence: 99%

Dextran hydrogels incorporated with bioactive glass-ceramic: Nanocomposite scaffolds for bone tissue engineering

Nikpour

Salimi-Kenari

Fahimipour

et al. 2018

Carbohydrate Polymers

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A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering. Scanning electron microscopy showed Dex/nBGC scaffolds were consisting of a porous 3D microstructure with an average pore size of 240 μm. Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents. It was found that the osmotic pressure and nBGC agglomeration at higher nBGC contents leads to increased water uptake, then reduction of the compressive modulus. Bioactivity of Dex/nBGC scaffolds was validated through apatite formation after submersion in the simulated body fluid. Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks. Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.

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In vitro proliferation and differentiation of human bone marrow mesenchymal stem cells into osteoblasts on nanocomposite scaffolds based on bioactive glass (64SiO 2 -31CaO-5P 2 O 5 )-poly- l -lactic acid nanofibers fabricated by electrospinning method

Cited by 60 publications

References 41 publications

Effects of GPTMS concentration and powder to liquid ratio on the flowability and biodegradation behaviors of 45S5 bioglass/tragacanth bioactive composite pastes

Effects of GPTMS concentration and powder to liquid ratio on the flowability and biodegradation behaviors of 45S5 bioglass/tragacanth bioactive composite pastes

Human mesenchymal stem cells differentiate into an osteogenic lineage in presence of strontium containing bioactive glass nanoparticles

Dextran hydrogels incorporated with bioactive glass-ceramic: Nanocomposite scaffolds for bone tissue engineering

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