In vitro Propagation and Essential Oils Composition with Cytotoxicity of Daucus capillifolius Gilli (Apiaceae)

Abstract

: Micropropagation and callus culture of Daucus capillifolius Gilli (Apiaceae) were successfully established in Murashige and Skoog(MS) solid medium enriched with different plant growth regulators. The combination of NAA (1mg/L) + BAP (0.1mg/L) was found to be the optimum for both organogenesis and embryogenesis. The compositions of essential oils obtained from wild fruits, cultivated fruits and callus culture were analyzed by GC-MS. The major identified constituents of the essential oils of wild and cultivated fruits were geranyl acetate (31.84%, 32.49%), α-azarone (21.69%), β-azarone (20.63 %), trans methyl isoeugenol (11.0%, 7.48%), α-humulene (7.25%, 4.35%), juniper camphor (4.71%, 4.26%) and α-pinene (3.26%, 3.63%), respectively. When screened, the essential oil of the wild fruits exhibited potent cytotoxic effect against Hep-G2 with IC5014.9 µg/mL and moderate activity against MCF-7 and HCT-116 cells with IC50 (29.1and 59.8 µg/mL), respectively. Meanwhile, the essential oil of the cultivated fruitsexhibited moderate activity against Hep-G2, 53.7 and 48.0 µg/mL), respectively.

UPLC-ESI-MS/MS Profiling of Secondary Metabolites from Methanol Extracts of In Vivo and In Vitro Tissues of Daucus capillifolius Gilli (A Comparative Study)

“…Callus was initiated from leaf explants of D. capillifolius seedlings as described by [ 19 ]. Excellent growth of calli with friable greenish white, friable bright yellow and compact yellowish white was obtained from calli grown on media A [M&S + NAA (1 mg/L) + BAP (0.1 mg/L)], B [M&S + TDZ (0.5 mg/L) + 2,4D (1 mg/L) + BAP (0.1 mg/L)] and C [M&S + 2,4 D (2 mg/L) + Kinetin (1 mg/L)], respectively ( Figure 1 ).…”

“…Chemically, Daucus is one of the richest sources of volatile oil, sterols and triterpenes, polyacetylenic compounds, flavonoids and sesquiterpene lactones. Biologically, the volatile oil of many species of this genus showed a wide range of important pharmacological activities, such as antioxidant, cytotoxicity, insecticidal, antimicrobial and anti-inflammatory activities [ 19 ]. Daucus capillifolius Gilli from Libya is a rare annual wild herb with an erect and smooth stem which reaches about 50 cm in length [ 18 ]…”

“…Based on the available literature, nothing has been reported on the phytochemical constituents of this plant, except our previous work, which investigated the micropropagation and callus culture of this endangered plant in addition to the GC-MS analysis of its essential-oil constituents [ 19 ]. The current study aimed to conduct a comparative phytochemical investigation of the methanolic extracts of in vivo (cultivated fruit) and in vitro tissues (calli grown on different media with various hormonal combinations) of this rare plant by using UPLC-ESI-MS/MS analysis in both ionization modes.…”