In vitro propagation from bud and apex explants of Moringa oleifera and evaluation of the genetic stability with RAMP marker

Ávila-Treviño, Jazmín A.; Muñoz-Alemán, J.M.; Pérez-Molphe-Balch, Eugenio; Rodríguez-Sahagún, Araceli; Morales-Domínguez, José Francisco

doi:10.1016/j.sajb.2016.10.003

Cited by 23 publications

(15 citation statements)

References 28 publications

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“…In organ and callus cultures, the shoot formation usually leads to genetic alterations, while genetic characteristics of clones can be preserved by propagation via shoot tips or meristem cultures (Bhojwani and Dantu, 2013). Numerous studies on different plants such as Arachis hypogaea (Hsieh et al, 2017), Moringa oleifera (Avila-Treviño et al, 2017), Camellia oleifera (Li et al, 2016), Ficus religiosa L. (Hesami et al, 2017), Passiflora caerulea L. (Jafari et al, 2017), and Withania somnifera (Nayak et al, 2013) have indi-cated that cotyledonary node explants possess a great potential for shoot proliferation. However, to date there have been limited studies on applying in vitro methods on plants from Chenopodiaceae family.…”

Section: Introductionmentioning

confidence: 99%

Optimizing sterilization conditions and growth regulator effects on in vitro shoot regeneration through direct organogenesis in Chenopodium quinoa

Hesami

Naderi

Yoosefzadeh-Najafabadi

2018

bta

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Chenopodium quinoa Willd. is known as a multipurpose crop that could be employed as an alternative on unsuitable lands with limited water resources such as arid and semi-arid regions. However, the biggest problem in cultivating this plant is contamination by viruses that are carried by quinoa seeds and cause diseases. Therefore, in vitro propagation methods could be applied to produce virus-free plants. The capacity of sodium hypochlorite to sterilize seeds and the regeneration potential of cotyledonary node segments were studied on Murashige and Skoog (MS) medium consisting of various concentrations of cytokinins and auxins either alone or in combinations. Although no contamination was observed after sterilization with 20% sodium hypochlorite and 10 and 15 min of immersion; with 25% sodium hypochlorite and 5, 10, and 15 min of immersion, the highest seed germination (100%) was obtained after sterilization with 20% sodium hypochlorite for 5 minutes. MS medium supplemented with 2.0 mg/l 6-benzylaminopurine (BAP) had the highest shoot induction frequency (93.33%) with an average of 4.96 shoots per node explant. This study is the first report of an appropriate and effective in vitro regeneration protocol for Chenopodium quinoa via direct organogenesis through cotyledonary node explants derived from axenic-seedlings, which can be effectively employed for the genetic manipulations and exploitation of active biomolecules of this valuable plant. This protocol can also increase the speed of quinoa breeding programs.

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Section: Introductionmentioning

confidence: 99%

Optimizing sterilization conditions and growth regulator effects on in vitro shoot regeneration through direct organogenesis in Chenopodium quinoa

Hesami

Naderi

Yoosefzadeh-Najafabadi

2018

bta

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“…The RAMP type markers have been used in studies of genetic diversity in several important cultivars and fruit plants (Dávila et al 1999;Cheng et al 2001;Zhang et al 2003;Yang et al 2006;Lihua et al 2013), but in Fusarium too (Alanís-Martínez et al 2015), and by Avila-Treviño et al (2017) who studied differentiation in plants propagated in vitro.…”

Section: Discussionmentioning

confidence: 99%

Easy strategy used to detect the genetic variability in chickpea (Cicer arietinum L.)

Valadez-Moctezuma

Cabrera-Hidalgo

2018

Physiol Mol Biol Plants

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A priority in the management and use of elite plant materials for breeding has been based on molecular markers or DNA sequencing of entire genomes, in order to perform genetic differentiation which is still quite costly. Chickpea () is one of the species with genomic monotony and very low polymorphism, and its detection even with DNA markers has not been easy. In germplasm banks, the genetic distinction is a priority in order to use properly selected lines. In this study, 57 chickpea accessions from a germplasm bank were analyzed by using nrRAMP (non-radioactive Random Amplified Microsatellite Polymorphism) markers, and their genetic variability was determined. Our results showed DNA polymorphisms, which are enough to differentiate between the accessions and between and (out-group); this last wild species is closely related to chickpea. We concluded that the nrRAMP technique was an effective and a highly useful method to assess the genetic diversity and variability among closely related plants, such as chickpea; in addition, this technique can be easily implemented in laboratories.

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“…This showed that the micropropagated Lagunaria patersonii is genetically stable. Many previous studies indicated the absence of genetic differences between parents and the micropropagated plants (Rout et al 2001;Nanda et al 2004;Bhatia et al 2011;Saha et al 2012;Esmaiel et al 2014;Agarwal et al 2015;Treviño et al 2017). The genetic variability through random amplified microsatellite polymorphism (RAMP) visualized as a dendrogram did not reveal any variations between the parent and the micropropagated plants in Moringa oleifera (Treviño et al 2017).…”

Section: Ssr Analysismentioning

confidence: 91%

Analysis of diversity using simple sequence repeat (SSR): distinctions between original Parmentiera cereifera tree and somaclones

El-Shafei

Esmaiel

2018

Bull Natl Res Cent

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Background: The possibility of culturing Parmentiera cereifera in vitro was tested. Shoot tips and lateral buds were cultured in three media that were based on Murashige and Skoog (MS) but supplemented with different types and concentrations of growth regulators. Thirty-eight simple sequence repeat (SSR) primers were used to assess the genetic stability of the regenerated plantlets.Results: Lateral buds recorded the highest significant mean values for shoot, root length, and the number of leaves when cultured in MS + 1.2 mg/l of 6-benzylaminopurine (BAP) + 1.5 g/l of activated charcoal. Seeds were also grown in different media. The best results were obtained with MS basal medium. The resulting shoots were rooted in MS medium, with 1.5 g/l of activated charcoal. Regenerated plants were acclimatized in the greenhouse. The 38 SSR primers produced 63 scorable bands ranging from 1 to 3, with an average of 1.68 per primer. Fifty-five monomorphic bands were obtained that ranged from 0 to 3, with an average of 1.45 per primer. The coefficient of similarity matrix ranged from 0.92 to 1.0, with an average of 97.4. Dendrogram generated using the SSR data tended to group the in vitro plants with the mother plant into two major clusters. The first cluster contained 19 in vitro plants with the mother plant and consisted of 4 subgroups. The second cluster contained in vitro plants, P-15, which had the lowest genetic similarity (92%) with the mother plant. Conclusions: The results revealed the increase in the degree of similarity between the tested plants in the SSR analyses. Therefore, micropropagation is a safe mode for multiplication of true-to-type plants of P. cereifera.

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In vitro propagation from bud and apex explants of Moringa oleifera and evaluation of the genetic stability with RAMP marker

Cited by 23 publications

References 28 publications

Optimizing sterilization conditions and growth regulator effects on in vitro shoot regeneration through direct organogenesis in Chenopodium quinoa

Optimizing sterilization conditions and growth regulator effects on in vitro shoot regeneration through direct organogenesis in Chenopodium quinoa

Easy strategy used to detect the genetic variability in chickpea (Cicer arietinum L.)

Analysis of diversity using simple sequence repeat (SSR): distinctions between original Parmentiera cereifera tree and somaclones

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